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Effectiveness of Menuzo's B2 Medium with Buffalo Rat Liver Cells for Development of In Vitro Matured/In Vitro Fertilized Bovine Oocytes

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Abstract

Purpose: The effectiveness of two culture media on the development of bovine embryos in a buffalo rat liver (BRL) coculture system was investigated.

Methods: Bovine oocytes were matured and fertilized in vitro, then cocultured, 25 per well, for 7 days in 500 μl of modified M199 or modified Menuzo's B2 medium over a BRL cell monolayer at 39°C in an atmosphere of 5% CO 2 in air. Medium 199 was modified by the addition of 10% of (v/v) fetal bovine serum (FBS), 9 mg/ml bovine serum albumin, 2 mM glycine, 1 mM alanine, and 0.1 mM nonessential amino acids (NEAA). Menuzo's B2 medium was modified by the addition of 10% (v/v) FBS, 1 mM alanine, and 0.1 mM NEAA.

Results: Modified Menuzo's B2 medium improved embryo development to the morula or blastocyst stage compared to modified M199 (121/353, 34.3%, versus 99/362, 27.3%, P<0.05).

Conclusions: These results suggest that Menuzo's B2 medium with modifications in a BRL coculture system can provide a significant benefit for culture of early bovine embryos over the traditional use of medium 199.

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Authors and Affiliations

  1. Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061-0315

    R. L. Krisher, J. R. Gibbons & F. C. Gwazdauskas

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  1. R. L. Krisher
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  2. J. R. Gibbons
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  3. F. C. Gwazdauskas
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Krisher, R.L., Gibbons, J.R. & Gwazdauskas, F.C. Effectiveness of Menuzo's B2 Medium with Buffalo Rat Liver Cells for Development of In Vitro Matured/In Vitro Fertilized Bovine Oocytes. J Assist Reprod Genet 15, 50–53 (1998). https://doi.org/10.1023/A:1022582422932

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  • DOI: https://doi.org/10.1023/A:1022582422932

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