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Suppression of Glial Tumor Growth by Expression of Glial Fibrillary Acidic Protein

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Abstract

We examined the effect of expression of glial fibrillary acidic protein (GFAP) on the tumor growth of astrocytoma in vivo. When rat astrocytoma C6 cells were injected subcutaneously in athymic mice, the cells produced tumors that grew rapidly. The tumor growth of C6 cells transfected with GFAP cDNA was significantly reduced compared to that of control NeoC6 cells transfected only with the neomycin resistant gene. After implantation of C6 cells transfected with mutated GFAP cDNA at the phosphorylation sites, the tumor growth was suppressed similar to that of the wild GFAP transfectants. To determine whether the cell growth suppression by GFAP is specific for astroglial cells, we assessed the effect of GFAP on the cell growth of an L cell of fibroblast origin in vitro. By GFAP cDNA transfection, L cells showed morphological changes, but the cell growth was not reduced. These results suggest that GFAP is a critical regulator of the tumor growth of astrocytoma.

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Authors and Affiliations

  1. Keio University School of Medicine, Shinjuku-ku, Tokyo, 160-8582, Japan

    Masahiro Toda & Keiichi Uyemura

  2. Departments of Neurosurgery, Keio University School of Medicine, Shinjuku, Tokyo, 160-8582, Japan

    Ichiro Sugiyama & Takeshi Kawase

  3. Department of Neuroanatomy, Biomedical Research Center, Osaka University Medical School, Suita, Osaka, 565, Japan

    Masayuki Miura

  4. Department of Neuro-cell Biology, Tokyo Metropolitan Institute of Gerontology, Itabashi, Tokyo, 173, Japan

    Hiroaki Asou

Authors
  1. Masahiro Toda
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  2. Masayuki Miura
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  3. Hiroaki Asou
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  4. Ichiro Sugiyama
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  5. Takeshi Kawase
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  6. Keiichi Uyemura
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Toda, M., Miura, M., Asou, H. et al. Suppression of Glial Tumor Growth by Expression of Glial Fibrillary Acidic Protein. Neurochem Res 24, 339–343 (1999). https://doi.org/10.1023/A:1022538810581

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