biologia plantarum

Biologia plantarum 46:7-12, 2003 | DOI: 10.1023/A:1022389428782

Changes in Sugars, Sucrose Synthase Activity and Proteins in Salinity Tolerant Callus and Cell Suspension Cultures of Brassica oleracea L.

O.C. Elavumoottil¹, J.P. Martín¹, M.L. Moreno¹

¹ Departmento Biología, ETSI Agrónomos, Universidad Politécnica, Madrid, Spain

Salt tolerant callus and cell suspension cultures of Brassica oleracea L. var. botrytis were obtained by the selection of cells from cultures growing in medium supplemented with 85, 170, and 255 mM NaCl. Salt adapted calli and cell suspensions differed in their RNA and protein concentrations. These concentrations tend to diminish in calli and increase in cell suspensions, both at one or three weeks periods of growth in NaCl. Contents of sucrose and reducing sugars, however, accumulate similarly both in calli and cell suspensions after NaCl treatments. The activity of sucrose synthase was higher in salt adapted cells than in controls. Calli exposed to 255 mM NaCl for six months synthesized a 27 kDa polypeptide, while a 13 kDa polypeptide present in control conditions was absent under salinity. Several high molecular mass polypeptides (> 200 kDa) were visualized in control calli and at moderate salt concentrations, when conditions of the gel were modified.

Keywords: adaptation; cauliflower; NaCl; RNA

Subjects: adaptation to salinity, in vitro culture, sucrose synthase; Brassica oleracea; cauliflower, in vitro culture, salinity tolerance; in vitro culture, callus, salinity tolerance; in vitro culture, salinity tolerance; in vitro culture, suspension culture; proteins, salinity tolerance; salt stress, in vitro culture, sucrose synthase; salt tolerance; sucrose synthase; sugars, salinity tolerance

Prepublished online: March 1, 2003; Published: July 1, 2003  Show citation