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Effects of Metallic Silver Particles on Resonance Energy Transfer Between Fluorophores Bound to DNA

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Abstract

We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4',6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å an to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Maryland Baltimore, Center for Fluorescence Spectroscopy, 725 W. Lombard St., Baltimore, Maryland, 21201

    Joseph R. Lakowicz, Yibing Shen, Sabato D'Auria, Zygmunt Gryczynski & Ignacy Gryczynski

  2. Faculty of Applied Physics and Mathematics, Technical University of Gdańsk, ul. Narutowicza 11/12, 80-952, Gdańsk, Poland

    Józef Kuśba & Joanna Malicka

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  1. Joseph R. Lakowicz
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  2. Józef Kuśba
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Correspondence to Joseph R. Lakowicz.

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Lakowicz, J.R., Kuśba, J., Shen, Y. et al. Effects of Metallic Silver Particles on Resonance Energy Transfer Between Fluorophores Bound to DNA. Journal of Fluorescence 13, 69–77 (2003). https://doi.org/10.1023/A:1022306630924

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