Skip to main content
SpringerLink
Log in

Solvent Relaxation of Prodan and Patman: A Useful Tool for the Determination of Polarity and Rigidity Changes in Membranes

  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

The time dependence of the solvent relaxation behavior of two polarity-sensitive dyes called Prodan and Patman has been investigated in detail in artificial membrane systems of different compositions. The works provides a comparison of the solvent relaxation behavior of the mentioned dyes, using steady-state and time-resolved fluorescence methods, and exemplifies their complementary use for membrane studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. M. R. Eftink (1992) in J. R. Lakowicz (Ed.), Topics in Fluorescence Spectroscopy: Principles, Plenum Press, New York, p. 53.

    Google Scholar 

  2. B. W. Van der Meer, G. Coker, and S. Y. S. Chen (1994) Resonance Energy Transfer Theory and Data, VCH Verlag, Weinheim.

    Google Scholar 

  3. C. D. Stubbs and B. W. Williams (1992) in J. R. Lakowicz (Ed.), Topics in Fluorescence Spectroscopy: Biochemical Applications, Plenum Press, New York, p. 231.

    Google Scholar 

  4. G. Duportail and P. Lianos (1996) in M. Rosoff (Ed.), Vesicles, Marcell Dekker, New York, p. 296.

    Google Scholar 

  5. G. Weber and F. Farris (1979) Biochemistry 18, 3075.

    Google Scholar 

  6. N. G. Bakshiev (1964) Opt. Spectrosc. (USSR) 16, 446.

    Google Scholar 

  7. Y. T. Mazurenko and N. G. Bakshiev (1970) Opt. Spectrosc. (USSR) 28, 490.

    Google Scholar 

  8. W. Liptay (1974) in E. C. Lim (Ed.), Excited States, Academic Press, New York, Vol. 1, p. 129.

    Google Scholar 

  9. M. L. Horng, J. A. Gardecki, A. Papazyan, and M. Maroncelli (1995) J. Phys. Chem. 99, 17320.

    Google Scholar 

  10. S. Mukherje and A. Chattopadhyay (1995) J. Fluoresc. 5, 237.

    Google Scholar 

  11. R. Hutterer, F. W. Schneider, N. Perez, H. Ruf, and M. Hof (1993) J. Fluoresc. 3, 257.

    Google Scholar 

  12. M. Hof, R. Hutterer, N. Perez, H. Ruf, and F. W. Schneider (1994) Biophys. Chem. 52, 165.

    Google Scholar 

  13. R. Hutterer, F. W. Schneider, H. Sprinz, and M. Hof (1996) Biophys. Chem. 61, 151.

    Google Scholar 

  14. R. Hutterer and M. Hof (1996) in J. Slavik (Ed.), Fluorescence Microscopy and Fluorescence Probes, Plenum Press, New York, p. 232.

    Google Scholar 

  15. R. Hutterer, F. W. Schneider, H. Lanig, and M. Hof (1997) Biochim. Biophys. Acta 1323, 195.

    Google Scholar 

  16. R. Hutterer, F. W. Schneider, V. Fidler, E. Grell, and M. Hof (1997) J. Fluoresc. 7, 161.

    Google Scholar 

  17. R. Hutterer, F. W. Schneider, and M. Hof (1997) J. Fluoresc. 7, 27.

    Google Scholar 

  18. J. B. Massey, H. S. She, and H. J. Powneall (1985) Biochemistry 24, 6973.

    Google Scholar 

  19. P. L. C. Chong (1988) Biochemistry 27, 399.

    Google Scholar 

  20. J. R. Lakowicz, D. R. Bevern, B. P. Maliwal, H. Cherek, and A. Balter (1983) Biochemistry 13, 5714.

    Google Scholar 

  21. A. Sommer, F. Paltauf, and A. Hermetter (1990) Biochemistry 29, 11134.

    Google Scholar 

  22. A. Hermetter, E. Prenner, J. Loidl, E. Kalb, A. Sommer, F. Paltauf (1993) in O. S. Wolfbeis (Ed.), Fluorescence Spectroscopy, Springer Verlag, Berlin, p. 149.

    Google Scholar 

  23. M. E. Jones and B. R. Lentz (1986) Biochemistry 25, 567.

    Google Scholar 

  24. S. W. Tendian and B. R. Lentz (1990) Biochemistry 29, 6720.

    Google Scholar 

  25. R. Hutterer, F. W. Schneider, W. Th. Hermens, R. Wagenvoord, and M. Hof (1988) Biochim. Biophys. Acta 1414, 155.

    Google Scholar 

  26. G. A. Cutsforth, R. N. Whittaker, and B. R. Lentz (1989) Biochemistry 28, 7453.

    Google Scholar 

Download references

Authors
  1. Rudi Hutterer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andreas B. J. Parusel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Martin Hof
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hutterer, R., Parusel, A.B.J. & Hof, M. Solvent Relaxation of Prodan and Patman: A Useful Tool for the Determination of Polarity and Rigidity Changes in Membranes. Journal of Fluorescence 8, 389–393 (1998). https://doi.org/10.1023/A:1020532817530

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1020532817530

Search

Navigation