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Presence of Platelet-Activating Factor and Its Receptor in Baboon (Papio spp) Spermatozoa

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Abstract

Platelet-activating factor [1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine; PAF] is a novel potent signaling phospholipid which has unique pleiotropic biological properties in addition to platelet activation. PAF has been detected in the spermatozoa in several species. The concentration of PAF is inversely related to human spermatozoal quality. PAF is present in squirrel monkey (a seasonal breeder) spermatozoa and is significantly higher during the breeding season. PAFs mechanism of action is a receptor-mediated event. There is no report on the presence of PAF or the PAF-receptor in nonhuman Old World primate spermatozoa. Therefore, the primary objective of this study was to determine if PAF is present in the spermatozoa from baboons, which are year-round breeders. A secondary objective was to determine the presence and localization of the PAF-receptor in spermatozoa. We extracted endogenous lipids from mature hybrid baboon (Papio spp) epididymal spermatozoa and assayed them for the presence of PAF by [ 125 I]-radioimmunoassay. We also exposed baboon spermatozoa to PAF-receptor antibody followed by FITC-conjugated antibody. PAF was in all samples assayed (mean: 2.29 (±0.63) pmol/10 6 spermatozoa). Baboon spermatozoa possess PAF-receptors most prevalently along the neck and midpiece regions. The data demonstrates that PAF and its receptor are present in baboon spermatozoa. Additional studies will elucidate the role of PAF in spermatozoal function.

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Authors and Affiliations

  1. Reproductive Primatology Laboratory, Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston, South Carolina

    William E. Roudebush (Ph.D.), Chikako Ito, Elissa T. Purnell & Xiaoying Cui

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  1. William E. Roudebush
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  2. Chikako Ito
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  3. Elissa T. Purnell
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  4. Xiaoying Cui
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Correspondence to William E. Roudebush.

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Roudebush, W.E., Ito, C., Purnell, E.T. et al. Presence of Platelet-Activating Factor and Its Receptor in Baboon (Papio spp) Spermatozoa. International Journal of Primatology 20, 273–280 (1999). https://doi.org/10.1023/A:1020526620855

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