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Phospholipase A2 Secretion During Intestinal Graft Ischemia

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Abstract

The time-dependent appearance of phospholipaseA2 (PLA2) activity in thepreservation media of ischemic rat intestinal grafts isdescribed. In controls, Ca2+-dependent,secretory PLA2 activity accumulated rapidly during the first 6 hr of ischemia,followed by a linear increase for up to 48 hr. LDHlevels, by contrast, increased linearly throughout the48 hr of ischemia. Addition of inhibitors ofPLA2, cyclooxygenase, and lipooxygenase blocked accumulation ofPLA2, but not LDH. PX-13, a novelPLA2 inhibitor, was most effective: 40 μMinhibited release by 86%, while 25 μM indomethacin(cyclooxygenase blocker) or nordihydroguiaretic acid (lipooxygenaseblocker) inhibited 41 and 36%, respectively. Thatappearance of PLA2 activity, but not LDH, isattenuated by inhibitors of the eicosanoid cascadesuggests a secretory event rather than leakage from dying cells. Thesecreted PLA2 is most likely theproinflammatory sPLA2 that has beenimplicated as a stress-induced protein and priming agentin ischemia-reperfusion injury.

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Authors
  1. Roberta E. Sonnino
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  2. Leslie Pigatt
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  3. Alexandra Schrama
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  4. Sonya Burchett
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  5. Richard Franson
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Sonnino, R.E., Pigatt, L., Schrama, A. et al. Phospholipase A2 Secretion During Intestinal Graft Ischemia. Dig Dis Sci 42, 972–981 (1997). https://doi.org/10.1023/A:1018876717308

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