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Use of Vaccinia Virus to Express Biopharmaceutical Products

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Abstract

Recent technological advancements have fostered the continued development of vaccinia virus as an efficient eukaryotic cloning and expression vector system. Genetically engineered vaccinia virus strains have been constructed for use (i) as recombinant vaccines for the prophylaxis of infectious disease, (ii) in producing significant quantities of biologically active polypeptide factors or enzymes, and (iii) as basic research tools with which to investigate primary structure–function relationships between proteins and their catalytic activities. This review examines the basic vaccinia vector system, its advantages and limitations, and current areas of research. As a specific example of the power and utility of this approach, attention is focused on the application of this technology to the field of neurobiology, specifically the use of recombinant vaccinia to study the expression, processing, and transport of cellular neuropeptides.

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Authors and Affiliations

  1. Department of Microbiology, Oregon State University, Corvallis, Oregon, 97331

    Dennis E. Hruby

  2. Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland, Oregon, 97201

    Gary Thomas

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  1. Dennis E. Hruby
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  2. Gary Thomas
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Hruby, D.E., Thomas, G. Use of Vaccinia Virus to Express Biopharmaceutical Products. Pharm Res 4, 92–97 (1987). https://doi.org/10.1023/A:1016454600151

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  • DOI: https://doi.org/10.1023/A:1016454600151

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