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Interaction of 65- and 62-kD Proteins from the Apical Membranes of the Aedes aegypti Larvae Midgut Epithelium with Cry4B and Cry11A Endotoxins of Bacillus thuringiensis

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Abstract

A protein with the molecular weight of 65 kD is the only component of Aedes aegypti larvae BBM capable to specifically bind mosquitocidal toxins Cry4B and Cry11A of Bacillus thuringiensis. This protein lacks the leucine aminopeptidase activity which is characteristic for the toxin-binding proteins from the membranes of caterpillars. Cry-toxins inactive against A. aegypti larvae either fail to bind to the 65-kD protein and to a putative product of its proteolysis with the molecular weight of 62 kD (Cry1Ab), or bind but do not compete for this binding with mosquitocidal proteins (Cry9A). The proteolytic splitting out of the first five α-helices in the Cry4B toxin molecule does not affect its binding to the 65- and 62-kD proteins, but an additional removal of 20-30 amino acids from the C-terminal of the molecule sharply spoils this binding. Monosaccharide residues are not involved in the binding of the 65- and 62-kD proteins with Cry4B, Cry11A, and Cry9A.

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REFERENCES

  1. Hofte, H., and Whiteley, H. (1989) Microbiol. Rev., 53, 242–255.

    Google Scholar 

  2. Crickmore, N., Zeigler, D. R., Feitelson, J., Schnepf, E., van Rie, J., Lereclus, D., Baum, J., and Dean, D. H. (1998) Microbiol. Mol. Biol. Rev., 62, 807–813.

    Google Scholar 

  3. Grochulski, P., Masson, L., Borisova, S., Pusztai-Carey, M., Schwartz, J.-L., Brousseau, R., and Cygler, M. (1995) J. Mol. Biol., 254, 447–464.

    Google Scholar 

  4. Hodgman, T. C., and Ellar, D. G. (1990) J. DNA Sequensis, 1, 97–106.

    Google Scholar 

  5. Li, J., Carrol, J., and Ellar, D. J. (1991) Nature, 353, 815–821.

    Google Scholar 

  6. Gill, S. S., Cowles, E. A., and Francis, V. (1995) J. Biol. Chem., 270, 27277–27282.

    Google Scholar 

  7. Knight, P. J. K., Crickmore, N., and Ellar, D. J. (1994) Mol. Microbiol., 11, 429–436.

    Google Scholar 

  8. Valaitis, A. P., Lee, M. K., Rajamohan, F., and Dean, D. H. (1995) Insect Biochem. Mol. Biol., 25, 1143–1151.

    Google Scholar 

  9. Valdamudi, R. K., Weber, E., Ji, I., Ji, T. H., and Bulla, L. (1995) J. Biol. Chem., 270, 5490–5494.

    Google Scholar 

  10. Krieger, I. V., Revina, L. P., Kostina, L. I., Buzdin, A. A., Zalunin, I. A., Chestukhina, G. G., and Stepanov, V. M. (1999) Biochemistry (Moscow), 64, 1163–1268.

    Google Scholar 

  11. Chestukhina, G. G., Kostina, L. I., Zalunin, I. A., Kotova, T. S., Katrukha, S. P., Kuznetsov, Yu. S., and Stepanov, V. M. (1977) Biokhimiya, 42, 1660–1667.

    Google Scholar 

  12. Chestukhina, G. G., Zalunin, I. A., Kostina, L. I., Bormatova, M. E., Klepikova, F. S., Khodova, O. M., and Stepanov, V. M. (1985) FEBS Lett., 190, 345–348.

    Google Scholar 

  13. Zalunin, I. A., Revina, L. P., Kostina, L. I., Chestukhina, G. G., and Stepanov, V. M. (1998) J. Prot. Chem., 17, 463–471.

    Google Scholar 

  14. Chestukhina, G. G., Kostina, L. I., Zalunin, I. A., Revina, L. P., Mikhailova, A. L., and Stepanov, V. M. (1994) Can. J. Microbiol., 40, 1026–1034.

    Google Scholar 

  15. MacIntosh, S. C., Lidster, B. D., and Kirkham, C. L. (1994) J. Invertebrate Pathol., 63, 97–98.

    Google Scholar 

  16. Wolfersberger, M., Luethy, P., Maurer, A., Parenti, P., Sacchi, F. V., Giordana, B., and Hanozet, G. M. (1987) Comp. Biochem. Physiol., 86A, 301–308.

    Google Scholar 

  17. Laemmli, U. K. (1970) Nature, 227, 680–685.

    Google Scholar 

  18. Bradford, M. M. (1976) Analyt. Biochem., 72, 248–254.

    Google Scholar 

  19. Hoopingarner, R., and Materu, M. E. A. (1964) J. Insect Pathol., 6, 26–30.

    Google Scholar 

  20. Bravo, A. (1997) J. Bacteriol., 179, 2793–2801.

    Google Scholar 

  21. Chestukhina, G. G., Tyurin, S. A., Kostina, L. I., Osterman, A. L., Zalunin, I. A., Khodova, O. A., and Stepanov, V. M. (1990) J. Prot. Chem., 9, 501–507.

    Google Scholar 

  22. Angsuthanasombat, C., Crickmore, N., and Ellar, D. J. (1993) FEMS Microbiol. Lett., 111, 255–262.

    Google Scholar 

  23. Muthukumar, G., and Nickerson, K. W. (1987) Appl. Environ. Microbiol., 53, 2650–2655.

    Google Scholar 

  24. Knowles, B. H., Knight, P. J. V., and Ellar, D. J. (1991) Proc. Roy. Soc. Lnd, B., 245, 31–35.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Genetics and Selection of Industrial Microorganisms, 1-yi Dorozhnyi Proezd 1, Moscow, 113545, Russia

    A. A. Buzdin, L. P. Revina, L. I. Kostina, I. A. Zalunin & G. G. Chestukhina

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  1. A. A. Buzdin
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  2. L. P. Revina
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  3. L. I. Kostina
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  4. I. A. Zalunin
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  5. G. G. Chestukhina
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Correspondence to I. A. Zalunin.

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Buzdin, A.A., Revina, L.P., Kostina, L.I. et al. Interaction of 65- and 62-kD Proteins from the Apical Membranes of the Aedes aegypti Larvae Midgut Epithelium with Cry4B and Cry11A Endotoxins of Bacillus thuringiensis . Biochemistry (Moscow) 67, 540–546 (2002). https://doi.org/10.1023/A:1015594127636

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