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Effect of phytohormones on growth and alkaloid accumulation by a Catharanthus roseus cell suspension cultures fed with alkaloid precursors tryptamine and loganin

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Abstract

This work presents a study of the effect of different phytohormones on growth and accumulation of terpenoid indole alkaloids in a Catharanthus roseus cell suspension culture upon feeding with the precursors loganin and tryptamine. The phytohormones tested were 2,4-dichlorophenoxyacetic acid, salicylic acid, methyl jasmonate and abscisic acid. Among these only methyl jasmonate enhanced the accumulation of alkaloids. Abscisic acid did not enhance the accumulation of alkaloids but delayed the catabolism of strictosidine.

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Authors and Affiliations

  1. Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    Magdi El-Sayed & Rob Verpoorte

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  1. Magdi El-Sayed
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  2. Rob Verpoorte
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Correspondence to Rob Verpoorte.

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El-Sayed, M., Verpoorte, R. Effect of phytohormones on growth and alkaloid accumulation by a Catharanthus roseus cell suspension cultures fed with alkaloid precursors tryptamine and loganin. Plant Cell, Tissue and Organ Culture 68, 265–270 (2002). https://doi.org/10.1023/A:1013968826635

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