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Processes Maintaining Calcium Homeostasis in Acinar Cells of the Rat Submandibular Salivary Gland

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Abstract

Using a Ca2+-sensitive fluorescent indicator, fura-2/AM, we recorded calcium transients in secretory cells of isolated acini of the rat submandibular salivary gland; these transients were induced by hyperpotassium-induced depolarization (after an increase in [K+] e up to 50 mM) of the plasma membrane of the above cells. Calcium transients were significantly suppressed by 50 μM nifedipine. Addition of 10 μM carbonyl cyanide m-chlorophenylhydrazone to the normal extracellular solution was accompanied by a rise in [Ca2+] i , whereas when hyperpotassium solution is used the effect was less expressed. Blockers of CA2+-ATPase in the cellular membrane and in the endoplasmic reticulum, eosin Y (5 μM) and cyclopiazonic acid (CPA, 5 μM), respectively, evoked a significant increase in [Ca2+] i and a decrease in the K+-depolarization-induced calcium transient. Extracellular application of caffeine (2, 10, or 30 mM) was accompanied by a concentration-dependent rise in [Ca2+] i . Therefore, potassium depolarization of the plasma membrane of acinar cells of the rat submandibular salivary gland activates both the voltage-dependent Ca2+ influx and Ca2+-induced Ca2+ release from the endoplasmic reticulum; the initial level of [Ca2+] i was restored at the joint involvement of Ca2+-ATPases in the plasma membrane and the membranes of the endoplasmic reticulum and mitochondria.

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Authors and Affiliations

  1. Franko National University, L'vov, Ukraine

    N. V. Fedirko & M. Yu. Klevets

  2. National Academy of Sciences of Ukraine, Bogomolets Institute of Physiology, Kyiv, Ukraine

    I. A. Kruglikov & N. V. Voitenko

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  1. N. V. Fedirko
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  2. M. Yu. Klevets
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  3. I. A. Kruglikov
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  4. N. V. Voitenko
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Fedirko, N.V., Klevets, M.Y., Kruglikov, I.A. et al. Processes Maintaining Calcium Homeostasis in Acinar Cells of the Rat Submandibular Salivary Gland. Neurophysiology 33, 216–223 (2001). https://doi.org/10.1023/A:1013568515451

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