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Direct fermentation of gelatinized sago starch to acetone–butanol–ethanol by Clostridium acetobutylicum

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Abstract

Direct fermentation of gelatinized sago starch into solvent (acetone–butanol–ethanol) by Clostridium acetobutylicum P262 was studied using a 250 ml Schott bottle anaerobic fermentation system. Total solvent production from fermentation using 30 g sago starch/l (11.03g/l) was comparable to fermentation using corn starch and about 2-fold higher than fermentation using potato or tapioca starch. At the range of sago starch concentration investigated (10–80 g/l), the highest total solvent production (18.82 g/l) was obtained at 50 g/l. The use of a mixture of organic and inorganic nitrogen source (yeast extract + NH4NO3) enhanced growth of C. acetobutylicum, starch hydrolysis and solvent production (24.47 g/l) compared to the use of yeast extract alone. This gave the yield based on sugar consumed of 0.45 g/g. Result from this study also showed that the individual concentrations of nitrogen and carbon influenced solvent production to a greater extent than did carbon to nitrogen (C/N) ratio.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    M.S. Madihah, A.B. Ariff, A.A. Suraini & M.I.A. Karim

  2. Fermentation Technology Centre, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    A.B. Ariff & K.M. Sahaid

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  1. M.S. Madihah
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  2. A.B. Ariff
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  3. K.M. Sahaid
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  4. A.A. Suraini
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  5. M.I.A. Karim
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Madihah, M., Ariff, A., Sahaid, K. et al. Direct fermentation of gelatinized sago starch to acetone–butanol–ethanol by Clostridium acetobutylicum. World Journal of Microbiology and Biotechnology 17, 567–576 (2001). https://doi.org/10.1023/A:1012351112351

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