Skip to main content
SpringerLink
Log in

Population structure and genetic variability of pearl oyster Pinctada mazatlanica along Pacific coasts from Mexico to Panama

  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

Populations of the Calafia pearl oyster Pinctada mazatlanica ofthe American Pacific coasts have been considered endangeredbecause of overfishing and/or alteration to coastal areas. Weassessed genetic variability and the pattern of populationstructure among 9 samples collected from Mexico to Panama, usingmtDNA RFLP analysis of two genes: 12S rRNA and subunit one ofCytochrome oxydase (COI). Haplotype diversity varied from 0.000to 0.856. The Panama population appeared to be monomorphic, whilethe other samples exhibited a level of haplotypic variabilitysimilar to those reported in the literature for the same kind ofanalysis on other bivalves species. A test for the impact ofdemographic history on genetic diversity was applied on thesequence data, and the results were congruent with a recentdecline of population sizes. Genetic differentiation was shown tofollow a scheme of isolation by distance, with low levels ofdifferentiation at the scales of ten to one hundred kilometres,whereas stronger and significant genetic structure was detectedat a larger scale. Three significantly distinct groups could thenbe defined, which correspond to Northern Mexico, Southern Mexico,and Panama.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Assmussen MA, Basten CJ (1996) Constraints and normalized measures for cytonuclear disequilibria. Hered., 76, 207-214.

    Google Scholar 

  • Belkhir K, Borsa P, Goudet J, Chikhi L, Bonhomme F (1996) GENETIX, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome et Populations, CNRS UPR 9060, Université de Montpellier II, Montpellier, France.

    Google Scholar 

  • Benzie JAH, Ballment E (1994) Genetic differences among black lipped pearl oyster (Pinctada margaritifera) populations in the western Pacific. Aquaculture, 127(2-3), 145-156.

    Google Scholar 

  • Bernatchez L, Glémet H, Wilson CC, Danzmann RG (1994) Introgression and Fixation of Arctic char (Salvelinus alpinus) mitochondrial genome in an allopatric population of brook trout (Salvelinus fontinalis). Can. J. Fish. Aquat. Sci., 52, 179-185.

    Google Scholar 

  • Bückle-Ramirez LF, Volotina-Lobina D, Morales-Guerrero E, Valenzuela-Buriel F (1992) Spat settlement and growth of Pteria sterna (Gould) (Mollusca, Bivalvia) in Bahia de Los Angeles, Baja California, Mexico. Trop. Ecol., 33(2), 13-14.

    Google Scholar 

  • Cariño M (1994) Natural pearl farming in the early century at Bahia de La Paz, South Baja California, Mexico. Abst. "International Pearl Conference" Hawaï, 346.

  • Durand P, Blanc F (1986) Divergence génétique chez un bivalve marin tropical: Pinctada margaritifera. Coll. Nat. CNRS "Biologie des populations", 323-330.

  • Galtier N, De Paulis F, Barton NH (2000) Detecting bottlenecks and selective sweeps from DNA sequence polymorphism. Genetics, 155, 981-987.

    Google Scholar 

  • Felsenstein J (1993) PHYLIP: Phylogeny Inference Package, version 3.5c.

  • Folmer O, Black M, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxydase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3(5), 294-299.

    Google Scholar 

  • Goodisman MAD, Shoemaker DW, Assmussen MA (1998) Cytonuclear Theory for haplodiploid species and X-linked genes. II. Stepping-stones models of gene flow and application to a fire ant hybrid zone. Evolution, 52(5), 1423-1440.

    Google Scholar 

  • Lewis RI, Thorpe JP (1994) Temporal stability of gene frequencies within genetically heterogeneous populations of queen scallop Aequipecten (Chlamys) opercularis. Mar. Biol., 121, 117-126.

    Google Scholar 

  • Li G, Hedgecock D (1998) Genetic heterogeneity, detected by PCR-SSCP, among samples of larval Pacific oysters (Crassostrea gigas) supports the hypothesis of large variance in reproductive success. Can. J. Fish. Aquat. Sci., 55, 1025-1033.

    Google Scholar 

  • Macaranas JM, Ablan CA, Pante MJR, Benzie JAH, Williams ST (1992) Genetic structure of giant clam (Tridacna derasa) populations from reefs in the Indo-Pacific. Mar. Biol., 113, 231-238.

    Google Scholar 

  • Mokady O, Rozenblatt S, Graur D, Loya Y (1994) Coral-host specificity of Red Sea Litophaga bivalves: Interspecific and intraspecific variation in 12S mitochondrial ribosomal RNA. Mol. Mar. Biol. Biotechnol., 3(3), 158-164.

    Google Scholar 

  • Monteforte M, Cariño M (1992) Exploration and Evaluation of Natural Stocks of Pearl Oysters Pinctada mazatlanica and Pteria sterna (Bivalvia: Pteriidae): La Paz Bay, South Baja California, Mexico. AMBIO, 21(4), 314-320.

    Google Scholar 

  • Moritz, C. (1994). Applications of mitochondrial DNA analysis in conservation: A critical review. Mol. Ecol., 3, 401-411.

    Google Scholar 

  • Nei M (1987) Molecular Evolutionary Genetics. Columbia University Press, New York.

    Google Scholar 

  • Norton JH, Dashorst M, Lansky TM, Mayer RJ (1996) An evaluation of some relaxants for use with pearl oysters. Aquaculture, 144, 39-52.

    Google Scholar 

  • Palumbi SR (1992) Marine speciation on a small planet. TREE, 7, 114-118.

    Google Scholar 

  • Palumbi SR, Wilson AC (1990) Mitochondrial DNA diversity in the sea Urchins Stongylocentrotus purpuratus and S. droebachiensis. Evolution, 44(2), 403-415.

    Google Scholar 

  • Planes S, Galzin R, Bonhomme F (1996). A genetic metapopulation model for reef fishes in oceanic islands: The case of the surgeonfish, Acanthurus triostegus. J. Evol. Biol., 9: 103-117.

    Google Scholar 

  • Rice WR (1989) Analysis tables of statistical tests. Evolution, 43, 223-225.

    Google Scholar 

  • Rousset F (1997) Genetic differenciation and estimation of gene flow from F-statistics under isolation by distance. Genetics, 145, 1219-1228.

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  • Saucedo P, Monteforte M, Bervera H, Perez V, Wright H (1994) Repopulation of natural beds of pearl oysters, Pinctada mazatlanica and Pteria sterna in Bahia de La Paz, South Baja California, Mexico. Abst. "International Pearl Conference" Hawaï, 347.

  • Saucedo P, Monteforte M (1997) Breeding cycle of pearl oysters Pinctada mazatlanica and Pteria sterna (Bivalvia: Pteriidae) at Bahia de La Paz, Baja California Sur, Mexico. J. Shellfish Res., 16(1), 103-110.

    Google Scholar 

  • Schneider S, Kueffer JM, Roessli D, Excoffier L (1997) ARLEQUIN 1.1: A Software for Population Genetic Data Analysis. Genetic and Biometry Laboratory, Department of Anthropology and Ecology, Université de Genè ve.

  • Singh-Cabanillas J, Bojó rquez-Verástica G, Meza-Domínguez JM (1982) Resultados finales de las actividades de estudios de ostras perleras en la Bahía de La Paz (1981-1982). Informe Técnico, Secretaría de Pesca, Delegació n Federal de Pesca, Oficina de Desarrollo Acuacultural, BCS, México. 52 pp.

    Google Scholar 

  • Tallman RF, Healey MC (1994). Homing, Straying and Gene Flow among Seasonally Separated Populations of Chum Salmon (Onchorynchus keta). Can. J. Fish. Aquat. Sci., 51, 5-588.

    Google Scholar 

  • VanSyoc RJ (1994) Genetic divergence between subpopulations of the eastern Pacific goose barnacle Pollicipes elegans: Mitochondrial cytochrome c subunit 1 nucleotides sequences. Mol. Mar. Biol. Biotechnol., 3(6), 338-346.

    Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of populations structure. Evolution, 38(6), 1358-1370.

    Google Scholar 

  • Wilson CC, Bernatchez L (1998) The ghost of hybrid past: Fixation of arctic charr (Salvelinus alpinus) mitochondrial DNA in an introgressed population of lake trout (S. namaycush). Mol. Ecol., 7(1), 127-132.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire ``Génome, Populations, Interactions'', Station Méditerranéenne de l'Environnement Littoral, 1, Quai de la Daurade, 34200, Sète, France

    Sophie Arnaud & François Bonhomme

  2. Cientro de Investigaciones Biologicas del Noroeste S.C., P.O. Box 128, La Paz, B.C.S., México

    Mario Monteforte

  3. Laboratoire ``Génome, Populations, Interactions'', CNRS UMR 5000 Université Montpellier II, CC 63 Place Eugène Bataillon, 34095, Montpellier, France

    Nicolas Galtier

  4. Laboratoire de Zoogéographie, Université Montpellier III, Route de Mende, 34199, Montpellier Cedex 5, France

    Françoise Blanc

Authors
  1. Sophie Arnaud
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mario Monteforte
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nicolas Galtier
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. François Bonhomme
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Françoise Blanc
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arnaud, S., Monteforte, M., Galtier, N. et al. Population structure and genetic variability of pearl oyster Pinctada mazatlanica along Pacific coasts from Mexico to Panama. Conservation Genetics 1, 299–308 (2000). https://doi.org/10.1023/A:1011575722481

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1011575722481

Search

Navigation