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A cDNA from grapevine (Vitis vinifera L.), which shows homology to AGAMOUS and SHATTERPROOF, is not only expressed in flowers but also throughout berry development

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Abstract

An AGAMOUS/SHATTERPROOF homologue (Vvmads1) was isolated from grapevine by differential display between berry and leaf mRNA. The predicted protein sequence of the full-length clone shows a high degree of homology to PLENA (77% identity) and to SHP1 and SHP2 (75% and 74% identity respectively), and is grouped with AGAMOUS/PLENA homologues when the conserved MADS and K domains are compared. Vvmads1 is expressed only in the later stages of flower development and throughout berry development, although expression is reduced after ripening commenced. When Vvmads1 was over-expressed in tobacco, the resulting plants display altered morphologies in the outer two floral whorls. In the most extreme cases, the inner whorls were surrounded by a carpelloid structure created by the modified sepals. Within these sepals were petals which had been split into sections and which were attached at the base of the flower by structures with the appearance of filaments. The results of this study suggest that Vvmads1 has a regulatory role in flower development before fertilisation and a role in fruit development after fertilisation.

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References

  • Barnard, C. and Thomas, J.E. 1933. Fruit bud studies. II. The Sul-tana: differentiation and development of the fruit buds. J. Coun. Sci. Ind. Res. (Aust) 6: 285–294.

    Google Scholar 

  • Boss, P.K., Davies, C. and Robinson, S.P. 1996. Analysis of the expression of anthocyanin pathway genes in developing Vitis.552 vinifera L. cv Shiraz grape berries and the implications for pathway regulation. Plant Physiol. 111: 1059–1066.

    Google Scholar 

  • Bouman, F. 1984. The ovule. In: B. Johri (Ed.) Embryology of Angiosperms, Springer-Verlag, Berlin, pp. 123–157.

    Google Scholar 

  • Bowman, J.L., Drews, G.N. and Meyerowitz, E.M. 1991. Expression of the Arabidopsis floral homeotic gene AGAMOUS is restricted to specific cell types late in flower development. Plant Cell 3: 749–758.

    Google Scholar 

  • Coombe, B.G. 1992. Research on development and ripening of the grape berry. Am. J. Enol. Vitic. 43: 101–110.

    Google Scholar 

  • Coombe, B.G. 1976. The development of fleshy fruits. Annu. Rev. Plant Physiol. 27: 207–228.

    Google Scholar 

  • Coombe, B.G. 1995. Adoption of a system for identifying grapevine growth stages. Aust. J. Grape Wine Res. 1: 100–110.

    Google Scholar 

  • Davies, C. and Robinson, S.P. 1996. Sugar accumulation in grape berries. Cloning of two putative vacuolar invertase cDNAs and their expression in grapevine tissues. Plant Physiol. 111: 275–283.

    Google Scholar 

  • Davies, C. and Robinson, S.P.2000. Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins. Plant Physiol. 122: 803–812.

    Google Scholar 

  • Drews, G.N., Bowman, J.L. and Meyerowitz, E.M.1991. Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product. Cell 65: 991–1002.

    Google Scholar 

  • Fan, H.Y., Hu, Y., Tudor, M. and Ma, H. 1997. Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins. Plant J. 12: 999–1010.

    Google Scholar 

  • Ferrandiz, C., Pelaz, S. and Yanofsky, M.F. 1999. Control of carpel and fruit development in Arabidopsis. Annu. Rev. Biochem. 68: 321–354.

    Google Scholar 

  • Flanagan, C.A., Hu, Y. and Ma, H. 1996. Specific expression of the AGL1 MADS-box gene suggests regulatory functions in Arabidopsis gynoecium and ovule development. Plant J. 10: 343–353.

    Google Scholar 

  • Gerrath, J.M. 1993. Developmental morphology and anatomy of grape flowers. Hort. Rev. 13: 315–337.

    Google Scholar 

  • Gleave, A.P. 1992. A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol. Biol. 20: 1203–1207.

    Google Scholar 

  • Gu, Q., Ferrandiz, C., Yanofsky, M.F. and Martienssen, R. 1998. The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development. Development 125: 1509–1517.

    Google Scholar 

  • Hardie, W.J., O'Brien, T.P. and Jaudzems, V.G. 1996. Morphology, anatomy and development of the pericarp after anthesis in grape, Vitis vinifera L. Aust. J. Grape Wine Res. 2:97–142.

    Google Scholar 

  • Horsch, R.B., Fry, J.T., Hoffmann, N.I., Eicholtz, D., Rogers, S.G. and Fraley, R.T.1985. A simple and general method for transforming genes into plants. Science 227: 1229–1231.

    Google Scholar 

  • Irish, V.F. and Sussex, I.M. 1990. Function of the APETALA-1 gene during Arabidopsis floral development. Plant Cell 2: 741–753.

    Google Scholar 

  • Kang, S.-G. and Hannapel, D.J. 1996. A novel MADS-box gene of potato (Solanum tuberosum L.) expressed during the early stages of tuberization. Plant Mol. Biol. 31: 379–386.

    Google Scholar 

  • Kang, H.G., Noh, Y.S., Chung, Y.Y., An, K.S. and An, G.H. 1995. Phenotypic alterations of petal and sepal by ectopic expression of a rice MADS-box gene in tobacco. Plant Mol. Biol. 29: 1–10.

    Google Scholar 

  • Kater, M.M., Colombo, L., Franken, J., Busscher, M., Masiero, S., Campagne, M.M.V. and Angenent, G.C. 1998. Multiple AGAMOUS homologs from cucumber and petunia differ in their ability to induce reproductive organ fate. Plant Cell 10: 171–182.

    Google Scholar 

  • Kempin, S.A., Mandel, M.A. and Yanofsky, M.F. 1993. Conversion of perianth into reproductive organs by ectopic expression of the tobacco floral homeotic gene NAG1. Plant Physiol. 103: 1041–1046.

    Google Scholar 

  • Liang, P. and Pardee, A.B. 1992. Differential display of eukaryotic mRNA by means of the polymerase chain reaction. Science 257: 967–971.

    Google Scholar 

  • Liljegren S.J., Ditta, G.S., Eshed, H.Y., Savidge, B., Bowman, J.L. and Yanofsky, M.F. 2000. SHATTERPROOF MADS-box genes control seed dispersal in Arabidopsis. Nature 404: 766–770.

    Google Scholar 

  • Liu, J.Y., Huang, Y.H., Ding, B. and Tauer, C.G. 1999. cDNA cloning and expression of a sweetgum gene that shows homology with Arabidopsis AGAMOUS. Plant Sci. 142: 73–82.

    Google Scholar 

  • Ma, H., Yanofsky, M.F. and Meyerowitz, E.M. 1991. AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes. Genes Dev. 5: 484–495.

    Google Scholar 

  • Mandel, M.A., Bowman, J.L., Kempin, S.A., Ma, H., Meyerowitz, E.M. and Yanofsky, M.F. 1992a. Manipulation of flower structure in transgenic tobacco. Cell 71: 133–143.

    Google Scholar 

  • Mandel, M.A., Gustafson-Brown, C., Savidge, B. and Yanofsky, M.F. 1992b. Molecular characterizaiton of the Arabidopsis floral homeotic gene APETALA1. Nature 360:273–277.

    Google Scholar 

  • May, P. and Antcliffe, A.J. 1973. The fruitfulness of grape buds. I. Measuring bud fruitfulness on forced single-node cuttings. Ann. Amélior. Plantes 23:1–12.

    Google Scholar 

  • Mizukami, Y., Huang, H., Tudor, M., Hu, Y. and Ma, H. 1996. Functional domains of the floral regulator AGAMOUS: charac-terization of the DNA binding domain and analysis of dominant negative mutations. Plant Cell 8: 831–845.

    Google Scholar 

  • Mizukami, Y. and Ma, H. 1992. Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell 71: 119–131.

    Google Scholar 

  • Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

    Google Scholar 

  • Okamoto, G., Konishi, Y. and Shimamura, K. 1977. The retardation of lateral shoot secondary growth and stimulation of inflorescence primordia development by spraying with CCC after harvest in Neo Muscat grapevines in a heated plastic green-house. Scientific Reports of the Faculty of Agriculture, Okayama University, pp. 21–26.

  • Pnueli, L., Hareven, D., Rounsley, S.D., Yanofsky, M.F. and Lifschitz, E. 1994. Isolation of the tomato AGAMOUS gene TAG1 and analysis of its homeotic role in transgenic plants. Plant Cell 6: 163–173.

    Google Scholar 

  • Pratt, C. 1971. Reproductive anatomy in cultivated grapes: a review. Am. J. Enol. Vitic. 22: 92–109.

    Google Scholar 

  • Purugganan, M.D., Rounsley, S.D., Schmidt, R.J. and Yanofsky, M.F. 1995. Molecular evolution of flower development: diversification of the plant MADS-box regulatory gene family. Genetics 140: 345–356.

    Google Scholar 

  • Rezaian, M.A. and Krake, L.R. 1987. Nucleic acid extraction and virus detection in grapevine. J. Virol. Meth. 17: 277–285.

    Google Scholar 

  • Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463–5467.

    Google Scholar 

  • Savidge, B., Rounsley, S.D. and Yanofsky, M.F. 1995. Temporal relationship between the transcription of two Arabidopsis MADS-box genes and the floral organ identity genes. Plant Cell 7: 721–733.

    Google Scholar 

  • Shore, P. and Sharrocks, A.D. 1995. The MADS-box family of transcription factors. Eur. J. Biochem. 229: 1–13..553

    Google Scholar 

  • Siebert, P.D., Chencnik, A., Kellogg, D.E., Lukyanov, K.A. and Lukyanov, S.A. 1995. An improved method for walking in uncloned genomic DNA. Nucl. Acids Res. 23: 1087–1088.

    Google Scholar 

  • Srinivasan, C. and Mullins, M.G. 1976. Reproductive anatomy of the grapevine (Vitis vinifera L.): origin and development of the anlage and its derivatives. Ann. Bot. 40: 1079–1084.

    Google Scholar 

  • Srinivasan, C. and Mullins, M.G. 1981. Physiology of flowering in the grapevine: a review. Am. J. Enol. Vitic. 32: 47–63.

    Google Scholar 

  • Tsuchimoto, S., van der Krol, A.R., Chua, N.H. and van der Krol, R. 1993. Ectopic expression of pMADS3 in transgenic petunia phenocopies the petunia blind mutant. Plant Cell 5: 843–853.

    Google Scholar 

  • Walden, A.R., Wang, D.Y., Walter, C. and Gardner, R.C. 1998. A large family of TM3 MADS-box cDNAs in Pinus radiata includes two members with deletions of the conserved K domain. Plant Sci. 138: 167–176.

    Google Scholar 

  • Weigel, D. and Meyerowitz, E.M. 1993. Activation of floral homeotic genes in Arabidopsis. Science 261: 1723–1726.

    Google Scholar 

  • Weigel, D. and Meyerowitz, E.M. 1994. The ABCs of floral homeotic genes. Cell 78: 203–209.

    Google Scholar 

  • West, A.G., Causier, B.E., Davies, B. and Sharrocks, A.D. 1998. DNA binding and dimerisation determinants of Antirrhinum majus MADS-box transcription factors. Nucl. Acids Res. 26: 5277–5287.

    Google Scholar 

  • Yanofsky, M.F., Ma, H., Bowman, J.L., Drews, G.N., Feldmann, K.A. and Meyerowitz, E.M. 1990. The protein encoded by the Arabidopsis homeotic gene AGAMOUS resembles transcription factors. Nature 346: 35–39.

    Google Scholar 

  • Zhang, H.M. and Forde, B.G. 1998. An Arabidopsis MADS-box gene that controls nutrient-induced changes in root architecture. Science 279: 407–409.

    Google Scholar 

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Authors and Affiliations

  1. CSIRO Plant Industry, Horticulture Unit, PO Box 350, Glen Osmond, SA, 5064, Australia

    Paul K. Boss, Ian B. Dry & Mark R. Thomas

  2. Department of Viticulture and Oenology, University of Stellenbosch, Private Bag XI, Matieland, 7062, Stellenbosch, South Africa

    Melané Vivier

  3. Department of Biology, Faculty of Education, Gifu University, Yanagido, Gifu, 501-1193, Japan

    Shogo Matsumoto

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  1. Paul K. Boss
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Boss, P.K., Vivier, M., Matsumoto, S. et al. A cDNA from grapevine (Vitis vinifera L.), which shows homology to AGAMOUS and SHATTERPROOF, is not only expressed in flowers but also throughout berry development. Plant Mol Biol 45, 541–553 (2001). https://doi.org/10.1023/A:1010634132156

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