biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:313-316, 2001 | DOI: 10.1023/A:1010232316425

Iron Deficiency Induced Changes on Electron Transport Rate in Pisum Sativum Chloroplasts

K. Muthuchelian1, M. Bertamini2, N. Nedunchezhian3,2
1 School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai-, India
2 Istituto Agrario di San Michele all'Adige, San Michele all'Adige, Italy
3 School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai-, India

Iron deficiency induced decrease in the rate of whole electron transport chain in chloroplasts of pea (Pisum sativum L.). Such reduction was mainly due to the loss of photosystem (PS) 2 activity. The same result was obtained when the ratio of variable to maximum chlorophyll fluorescence (Fv/Fm) was evaluated. The loss in PS 2 activity was primarily due to a loss of 33, 23 and 17 kDa polypeptides. In contrast, iron deficiency induced the synthesis of 28 and 29 kDa polypeptides.

Keywords: fluorescence; light harvesting chlorophyll-protein complexes; pea; photosystem
Subjects: chlorophylls, iron defiency; chloroplast, iron deficiency; electron transport rate, chloroplast, iron deficiency; fluorescence, chlorophyll; iron deficiency, chloroplast, electron transport rate; light-harvesting chlorophyll-protein complexes, iron defiency; pea; photosystem 2, iron defiency; Pisum sativum

Published: June 1, 2001  Show citation

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Muthuchelian, K., Bertamini, M., & Nedunchezhian, N. (2001). Iron Deficiency Induced Changes on Electron Transport Rate in Pisum Sativum Chloroplasts. Biologia plantarum, 44(2), 313-316. doi: 10.1023/A:1010232316425
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    References

    1. Abadia, A., Lemoine, Y., Tremolieres, A., Ambard Bretteville, F., Remy, R.: Iron deficiency in pea: effects on pigment, lipid and pigment complex composition of thylakoids.-Plant Physiol. Biochem. 27: 679-687, 1989.
    2. Abadia, J., Morales, F., Abadia, A.: Photosystem II efficiency in low chlorophyll, iron deficient leaves.-Plant Soil 215: 183-192, 2000.
    3. Alcaraz, C.F., Hellin, E., Sevilla, F., Martinez-Sanchez, F.: Influence of the leaf iron contents on the ferredoxin levels in citrus plants.-J. Plant Nutr. 8: 603-611, 1985. Go to original source...
    4. Behrenfeld, M.J., Bale, A.J., Kolber, Z.S., Aiken, J., Falkowski, P.G.: Confirmation of iron limitation of phytoplankton photosynthesis in the equatorial Pacific ocean.-Nature 383: 508-511, 1996. Go to original source...
    5. Enami, I., Kitamura, M., Tomo, T., Isokawa, Y., Ohta, H., Katoh, S.: Is the primary cause of thermal inactivation of oxygen evolution in spinach PS II membranes release of the extrinsic 33 kDa protein or of Mn?-Biochim. biophys. Acta 1186: 52-58, 1994. Go to original source...
    6. Foder, F., Boddi, B., Sarvari, E., Zaray, G., Cseh, E., Lang, F.: Correlation of iron content, spectral forms of chlorophyll and chlorophyll-proteins in iron deficient cucumber (Cucumis sativus).-Physiol. Plant. 93: 750-756, 1995. Go to original source...
    7. Kuwabara, T., Miyao, M., Murata, T., Murata, N.: The function of 33-kDa protein in the photosynthetic O2 evolution system studied by reconstitution experiments.-Biochim. biophys. Acta 806: 283-289, 1985. Go to original source...
    8. Laemmli, U.K.: Clevage of structural proteins during the assembly of head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    9. Lichtenthaler, H.K.: Chlorophylls and carotenoids - pigments of photosynthetic biomembranes.-Methods Enzymol. 148: 350-382, 1987. Go to original source...
    10. Lien, S., Bannister, T.T.: Multiple sites of DCPIP reduction by sonicated oat chloroplasts. Role of plastocyanin.-Biochim. biophys. Acta 245: 465-481, 1971. Go to original source...
    11. Ljungberg, U., Akerlund, C., Larsson, C., Andersson, B.: The release of a 10 kDa polypeptide from everted photosystem II thylakoid membranes by alkaline Tris.-FEBS Lett. 175: 255-258, 1984. Go to original source...
    12. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    13. Millner, P.A., Gogel, G., Barber, J.: Investigation of the spatial relationship between photosystem 2 polypeptides by reversible crosslinking and diagnonal electrophoresis.-Photosynth. Res. 13: 185-198, 1987. Go to original source...
    14. Misra, A., Srivastava, N.K.: Influence of iron nutrition on chlorophyll contents, photosynthesis and essential monoterpene oil(s) in Java citronella (Cymbopogon winterianus Jowitt).-Photosynthetica 30: 425-434, 1994.
    15. Morales, F., Abadia, A., Abadia, J.: Characterization of the xanthophyll cycle and other photosynthetic pigment changes induced by iron deficiency in sugar beet (Beta vulgaris L.).-Plant Physiol. 94: 607-613, 1990. Go to original source...
    16. Morales, F., Abadia, A., Abadia, J.: Photosynthesis, quenching of chlorophyll fluorescence and thermal energy dissipation in iron-deficient sugar beet leaves.-Aust. J. Plant Physiol. 25: 403-412, 1998. Go to original source...
    17. Morales, F., Belkhoja, R., Abadia, A., Abadia, J.: Photosystem II efficiency and meachanism of energy dissipation in irondeficient, field grown pear trees (Pyrus communis L.).-Photosynth. Res. 63: 9-21, 2000. Go to original source...
    18. Mortvedt, J.J.: Correcting iron deficiencies in annual and perenial plants: Present technologies and future prospects.-Plant Soil 130: 273-279, 1991. Go to original source...
    19. Murata, N., Miyao, M., Omata, T., Matsunami, H., Kuwabara, T.: Stoichiometry of components in the photosynthetic O2 evolution system of photosystem II particles prepared with Triton X-100 from spinach chloroplasts.-Biochim. biophys. Acta 765: 363-369, 1984. Go to original source...
    20. Nedunchezhian, N., Kulandaivelu, G.: Evidence for the ultraviolet-B (280-320 nm) radiation induced structural reorganization and damage of photosystem II polypeptides in isolated chloroplasts.-Physiol. Plant. 81: 558-562, 1991. Go to original source...
    21. Nedunchezhian, N., Morales, F., Abadia, A., Abadia, J.: Decline in photosynthetic electron transport activity and changes in thylakoid protein pattern in field grown iron deficient peach (Prunus persica L.).-Plant Sci. 129: 29-38, 1997. Go to original source...
    22. Ouitrakul, R., Izawa, S.: Electron transport and photophosphorylation in chloroplasts as a function of the electron acceptor II Acceptor-specific inhibition by KCN.-Biochim. biophys. Acta 305: 105-118, 1973. Go to original source...
    23. Packham, N.K., Mansfield, R.W., Barber, J.: Action of cyanide on the photosynthetic water splitting process.-Biochim. biophys. Acta 681: 538-541, 1982. Go to original source...
    24. Platt-Aloia, K.A., Thomson, W.W., Terry, N.: Changes in plastid ultrastructure during iron nutrition-mediated chloroplast development.-Protoplasma 114: 85-92, 1983. Go to original source...
    25. Pushnik, J.C., Miller, G.W.: The effects of iron and light treatments on chloroplast composition and ultrastructure in iron deficient barley leaves.-J. Plant Nutr. 5: 311-322, 1982. Go to original source...
    26. Riethman, H.C., Sherman, L.A.: Purification and characterisation of an iron stress-induced chlorophyll-protein from cyanobacteria Anacystis nidulans R2.-Biochim. biophys. Acta 935: 141-151, 1988. Go to original source...
    27. Sharma, S., Sanwal, G.G.: Effect of Fe deficiency on the photosynthetic system of maize.-J. Plant Physiol. 140: 527-530, 1992. Go to original source...
    28. Spiller, S.C., Terry, N.: Limiting factors in photosynthesis. II. Iron stress diminishes photochemical capacity by reducing the number of photosynthetic units.-Plant Physiol. 65: 121-125, 1980. Go to original source...
    29. Stocking, C.R.: Iron deficiency and the structure and physiology of maize chloroplasts.-Plant Physiol. 55: 626-631, 1975. Go to original source...
    30. Straus, N.A.: Iron deprivation: Physiology and gene regulation.-In: Bryant, D.A. (ed.): The Molecular Biology of Cyanobacteria. Pp. 731-750, Kluwer Academic Publishers, Dordrecht-Boston-Lancaster 1994. Go to original source...
    31. Terry, N.: Limiting factors in photosynthesis. I. Use of iron stress to control photochemical capacity in vivo.-Plant Physiol. 65: 114-120, 1980. Go to original source...
    32. Terry, N., Abadia, J.: Function of iron chloroplasts.-J. Plant Nutr. 9: 609-646, 1986. Go to original source...

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