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The distribution of somatic H1 subtypes is non-random on active vs. inactive chromatin: Distribution in human fetal fibroblasts

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Abstract

Chromatin immunoprecipitation was employed to determine whether or not the previously reported depletion of histone H1 on actively transcribed sequences was selective with respect to H1 subtypes. DNA of immunofractionated chromatin was analyzed by slot-blots for repetitive sequences and PCR for single and low-copy sequences. Based on the analysis of a diverse set of sequences, we report distinct differences in subtype distributions. Actively transcribed chromatin, as well as chromatin poised for transcription, is characterized by a relative depletion of somatic H1 subtypes 2 and 4 (H1s-2 and H1s-4), whereas facultative and constitutive heterochromatin contain all four somatic subtypes. These results support a model in which subtypes are selectively depleted upon gene expression. In turn, the data also support the possibility that the somatic subtypes have different functional roles based on their selective depletion from different classes of DNA sequences.

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Authors and Affiliations

  1. Research and Development, Techniclone Corporation, 14282 Franklin Ave, Tustin, CA, 92780

    Missag H. Parseghian

  2. The UCI Center for Statistical Consulting, University of California, Irvine 3205 Bio. Sci. II, Irvine, CA, 92697

    Robert L. Newcomb

  3. Department of Biological Chemistry, University of California, Irvine 3205 Bio. Sci. II, Irvine, CA, 92697

    Sara T. Winokur

  4. Department of Molecular Biology and Biochemistry, University of California, Irvine 3205 Bio. Sci. II, Irvine, CA, 92697.

    Barbara A. Hamkalo

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  1. Missag H. Parseghian
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  2. Robert L. Newcomb
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  4. Barbara A. Hamkalo
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Parseghian, M.H., Newcomb, R.L., Winokur, S.T. et al. The distribution of somatic H1 subtypes is non-random on active vs. inactive chromatin: Distribution in human fetal fibroblasts. Chromosome Res 8, 405–424 (2000). https://doi.org/10.1023/A:1009262819961

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