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Evaluation of genotoxic and immunotoxic activities of potential glucose biosensor components: ferrocenes

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Abstract

Three ferrocenes used in glucose biosensor construction were tested in the aspect of genotoxic and immunotoxic activities. All three ferrocenes were not mutagenic in the standard bacterial Ames test. Equally in the Sister Chromatid Exchanges test in human lymphocyte cultures, the genotoxic action of tested ferrocenes could be excluded. However, all three significantly decreased the rate of lymphocyte proliferation and especially diminished the numbers of B-lymphocytes and NK-cells after 72 hours of in vitro culture. Marked differences between the ferrocenes in their immunotoxic activities were noticed, and we were able to select those which would be relatively safe and those which should be avoided in further investigation of the glucose biosensor construction. Our results indicate the necessity to estimate immunotoxic effects as well as genotoxic effects, especially in biosensor components potentially used in vivo

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References

  • Ames BN, McCann J, Yamasaki E. 1975 Methods for detecting carcinogens and mutagens with the Salmonella/mammalian — microsome mutagenicity test. Mutat Res 31, 347–364.

    Google Scholar 

  • Ames BN, Gold LS. 1996 Mutagenesis and carcinogenesis: Cell division and DNA lesions as key factors. In: Bertino JR, ed. Encyclopedia of Cancer (Academic Press, Inc., Orlano, FL) 2, 1120–1128.

    Google Scholar 

  • Antoshina MN, Poriadkova NA. 1978 A technique for differential staining of sister chromatids without using fluorochrome. Citol Genet 4, 349–352.

    Google Scholar 

  • Albery WJ, Barlett PN, Craston DH. 1985 Conducting salts as electrode materials for the oxidation of glucose oxidase. J Electroanal Chem 194, 223–235.

    Google Scholar 

  • Boutelle MG, Stanford C, Fillenz M, Albery WJ, Bartlett PN. 1986 An amperometric enzyme electrode for monitoring brain glucose in the freely moving rat. Neurosci Lett 72, 283–288.

    Google Scholar 

  • Campbell MJ, Machin D. 1993 Correlation and regression. In: Campbell MJ, Machin D, eds. Medical Statistics. A commonsense Approach. Chichester, New York, Brisbane, Toronto, Singapore: John Wiley & Sons 87–103.

    Google Scholar 

  • Carrano AV, Thompson LH, Lind PA, Minkler JL. 1978 Sister chromatid exchanges as an indicator of mutagenesis. Nature. London 271, 551–553.

    Google Scholar 

  • Cass AEG, Davis G, Francis GD. 1984 Ferrocene-mediated glucose electrodes for amperometric determination of glucose. Anal Chem 56, 667–671.

    Google Scholar 

  • Cooper J, Bannister JV, McNeil CJ. 1991 A kinetic study of the catalyzed oxidation of 1′,3-dimethyl-ferrocene ethylamine by cytochrome c peroxidase. J Electroanal Chem 312, 155–161.

    Google Scholar 

  • Cunningham ML, Matthews HB. 1995 Cell proliferation as a determining factor for the carcinoge-nicity of chemicals: studies with mutagenic carcinogens and mutagenic noncarcinogens. Toxicol Letters 82, 9–14.

    Google Scholar 

  • English D, Anderson BR. 1997 Single-step separation of blood cells. Granulocytes and mono-nuclear leucocytes on discontinuous density gradient of Ficoll-Hypaque. J Immunol Methods 5, 249–254.

    Google Scholar 

  • Franceschi C. 1990 Genomic istability: a challenge for aging research. Aging 2, 101–104.

    Google Scholar 

  • Franceschi C, Monti D, Scarfi MD, Zeni O, Temperani GE, Sansoni P, Lioi MB, Troianoel L, Agnesini C, Salvidi S, Cossarizza A. 1992 Genomic instability and aging. Ann NY Acad Sci 663, 4–18.

    Google Scholar 

  • Fraser DM, Zakeeruddin SM, Graetzel M. 1993 Towards mediator design. II. Optimization of mediator global charge for the mediation of glucose oxidation of Aspergillus niger. J Elektroanal Chem 359, 125–139.

    Google Scholar 

  • Fraser DM. 1997 Biosensor in the Body: Continuous in vivo Monitoring. Fraser DM, ed. Chichester: John Wiley & Sons.

    Google Scholar 

  • Galloway SM, Bloom AD, Resnick M. 1985 Development of a standard protocol for in vitro cyto-genetic testing with Chinese hamster ovary cells. Environ Mutagen 7, 1–51.

    Google Scholar 

  • Gąsiorowski K, Szyba K, Urban J, Cielak-Golonka M, Zakeeruddin SM, Graetzel M, Fraser DM. 1995 Mutagenic activity of group VIII metal-organic complexes in the Ames test: evaluation of potential glucose biosensor components. BioMetals 8, 257–262.

    Google Scholar 

  • Haworth S, Lawlor T, Mortelmans K, Speck W, Ziegler E. 1983 Salmonella mutagenicity test results for 250 chemicals. Environ Mutagen 5(Suppl 1), 3–142.

    Google Scholar 

  • Hsu SM, Raine L, Fanger H. 1981 Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase technique: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histo-chem Cytochem 29, 577–580.

    Google Scholar 

  • Kanda N. 1982 Spontaneous sister chromatid exchange in vivo. In: Sandberg AA, ed. Progress and Topics in Cytogenetics 2. New York: Liss 279–296.

    Google Scholar 

  • Knutilla S, Teerenhovi L. 1989 Immunophenotyping of aneuploid cells. Cancer Genet Cytogenet 41, 1–17.

    Google Scholar 

  • Kulys JJ, Cenas NK. 1983 Oxidation of glucose oxidase from Penicillium vitale by one and two-electron acceptors. Biochim Biophys Acta 744, 57–63.

    Google Scholar 

  • Leung H-W, Hallesy DW, Shott LD, Murray FJ, Paustenbach DJ. 1987 Toxicological evaluation of substituted dicyclopentadienyliron (ferrocene) compounds. Toxicol Lett 38, 103–108.

    Google Scholar 

  • Linke B, Kerner K, Kiwit M, Pishko M, Heller A. 1994 Amperometric biosensor for in vivo glucose sensing, based on glucose oxidase immobilized in a redox hydrogel. Biosens Bioelectron 9, 151–158.

    Google Scholar 

  • Marcher H, Haeggqvist I, Karlsson B, Johansson T. 1988 Studies on the mutagenic effects of ferrocene carbamate and H1–6, two candidate antidotes against Soman poisoning. Swedish Government Reports, Announcements and Index (GRA&I) 2.

  • Maron DM, Ames BN. 1983 Revised method for the Salmonella mutagenicity test. Mutat Res 113, 173–215.

    Google Scholar 

  • Matthews DR, Brown E, Beck TW et al. 1988 An amperometric needle-type glucose sensor tested in rats and man. Diabetic Med 5, 248–253.

    Google Scholar 

  • Nikula KJ, Sun JD, Barr EB. 1993 Thirteen-week, repeated inhalation exposure of E 344/N rats and B6C3F1 mice to ferrocene. Fund Appl Toxicol 21, 127–139.

    Google Scholar 

  • O'Neill JP, Heartlein MW, Preston RJ. 1983 Sister chromatid exchanges and gene mutation are induced by replication of 5-bromo-and 5-chloro-deoxyuridine substituted DNA. Mutat Res 109, 259–270.

    Google Scholar 

  • Perry P, Wolffs S. 1974 New Giemsa method for the differential staining of sister chromatids. Nature 251, 156–159.

    Google Scholar 

  • Perry PE. 1980 Chemical mutagens and sister chromatid exchanges. In: De Serres FJ, Holloender A, ed. Chemical Mutagens vol 6. New York: Plenum Press 1–39.

    Google Scholar 

  • Pinero J, Ortiz T, Cortes F. 1993 Three-way differentiation of Chinese Hamster Ovary chromosomes by immunoperoxidase technique using a monoclonal anti-bromodeoxyuridine antibody. Biotech Histochem 68, 215–221.

    Google Scholar 

  • Sakakida M, Nishida K, Shichiri M, Ishihara K, Nakabayashi N. 1993 Ferrocene-mediated needle-type glucose sensor covered with newly-designed biocompatible membrane. Sens Act B 13–14, 319–322.

    Google Scholar 

  • Sasaki MS. 1977 Sister chromatid exchange and chromatid interchange as possible manifestation of different DNA repair processes. Nature (London) 269, 623–625.

    Google Scholar 

  • Schuhmann W, Wohlschlger H, Lammert R, et al. 1990 Leaching of dimethylferrocene, a redox mediator in amperometric enzyme electrodes. Sens Act B 1, 571–575.

    Google Scholar 

  • Tomatis L. 1993 Cell proliferation and carcinogenesis: A brief history and current view based on an IARC Workshop Report. Environ Health Perspectives 101(Suppl 5), 149–152.

    Google Scholar 

  • Tucker JD, Christensen ML, Strout CL, Carrano AV. 1986 Determination of the baseline sister chromatid exchange frequency in human and mouse peripheral lymphocytes using monoclonal antibodies and very low doses of bromodeoxyuridine. Cytogenet Cell Genet 43, 38–42.

    Google Scholar 

  • Warnke R, Levy R. 1980 Detection of T and B cell antigens with hybridoma monoclonal antibodies. A biotinavidin-horseradish peroxidase method. J Histochem Cytochem 28, 771–776.

    Google Scholar 

  • Zakeeruddin SM, Fraser DM, Nazeeruddin M-K, Graetzel M. 1992 Towards mediator design: characterization of tris-(4,4′-substituted-2,2′-bipyridine) complexes of iron (II), ruthenium (II) and osmium (II) as mediators for glucose oxidase of Aspergillus niger and other redox proteins. J Electroanal Chem 337, 253–283.

    Google Scholar 

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Gasiorowski, K., Brokos, J., Szyba, K. et al. Evaluation of genotoxic and immunotoxic activities of potential glucose biosensor components: ferrocenes. Biometals 12, 19–26 (1999). https://doi.org/10.1023/A:1009238611562

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