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Selection of a strain of Aspergillus for the production of citric acid from pineapple waste in solid-state fermentation

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Abstract

Aspergillus foetidus ACM 3996 (=FRR 3558) and three strains of Aspergillus niger ACM 4992 (=ATCC 9142), ACM 4993 (=ATCC 10577), ACM 4994 (=ATCC 12846) were compared for the production of citric acid from pineapple peel in solid-state fermentation. A. niger ACM 4992 produced the highest amount of citric acid, with a yield of 19.4g of citric acid per 100g of dry fermented pineapple waste under optimum conditions, representing a yield of 0.74g citric acid/g sugar consumed. Optimal conditions were 65% (w/w) initial moisture content, 3% (v/w) methanol, 30°C, an unadjusted initial pH of 3.4, a particle size of 2mm and 5ppm Fe2+. Citric acid production was best in flasks, with lower yields being obtained in tray and rotating drum bioreactors.

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Authors and Affiliations

  1. Department of Chemical Engineering, The University of Queensland, Brisbane, Queensland, 4072, Australia; fax

    C.T. Tran & D.A. Mitchell

  2. Km 8 Nguyen Trai Rd, Dong Da, Hanoi, Vietnam; fax

    C.T. Tran

  3. Department of Microbiology, The University of Queensland, Brisbane, Queensland, 4072, Australia; fax

    L.I. Sly

Authors
  1. C.T. Tran
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  2. L.I. Sly
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  3. D.A. Mitchell
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Tran, C., Sly, L. & Mitchell, D. Selection of a strain of Aspergillus for the production of citric acid from pineapple waste in solid-state fermentation. World Journal of Microbiology and Biotechnology 14, 399–404 (1998). https://doi.org/10.1023/A:1008821413239

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  • DOI: https://doi.org/10.1023/A:1008821413239

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