Abstract
A novel in vitro bioassay is described for screening Fusarium ear blight (FEB) resistance in adult winter wheat plants. Seven winter wheat cultivars were assessed for components of partial disease resistance as 28 day-old detached leaf segments in the laboratory using isolates of Microdochium nivale var. nivale and M. nivale var. majus. Results were compared with disease data obtained at anthesis using the same cultivars as whole plants and the same isolates under glasshouse conditions. Significant cultivar differences were observed using detached leaves, with cv. Avalon (a Fusarium culmorum ear susceptible cultivar) having the shortest leaf incubation period, greatest leaf lesion development and shortest leaf latent period compared to cv. Spark (a Fusarium culmorum ear resistant cultivar), which had the longest leaf incubation period, least leaf lesion development and longest leaf latent period. Using whole plants, cv. Avalon had the shortest ear incubation period and greatest ear disease severity, whilst cv. Spark had the longest incubation period and least ear disease severity. Overall, cultivars of intermediate F. culmorum ear resistance expressed intermediate responses to M. nivale isolates, using both detached leaves and whole plants. Significant correlations were found with ear disease severity and ear incubation period in whole plants and components of partial disease resistance in detached leaves, with significant correlations obtained between leaf incubation period and ear disease parameters using the M. nivale var. nivale isolate. In addition, leaf latent period and leaf lesion size showed significant correlations with whole plant reactions using M. nivale var. nivale and var. majus isolates. The in vitro screening of cultivars as detached leaves using M. nivale isolates may offer a real possibility of a rapid bioassay for the early screening of FEB resistance in wheat and other cereals.
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References
Ablova IB and Slusarenko AN (1996) The problems associated with breeding winter wheat for head scab resistance. In: Proceedings of the Workshop on Fusarium Head Scab: Global Status and Future Prospects, CIMMYT, Mexico, pp 93-96
Arraiano LS, Brading PA and Brown JKM (1998) A detached seedling leaf technique to screen for resistance to Mycosphaerella graminicola (Septoria tritici) in wheat varieties. 7th International Congress of Plant Pathology Abstracts Vol. 3, 3.4.52
Baker EA and Smith JM (1978) Development of resistant and susceptible reactions in wheat on inoculation with Septoria nodorum. Transactions of the British Mycological Society 71: 475-482
Benedikz PW, Mappledoram CJ and Scott PR (1981) A laboratory technique for screening cereals for resistance to Septoria nodorum. Transactions of the British Mycological Society 77: 667-668
Bruehl GW (1967) Fusarium headblight or scab. In: Reitz LP and Queensbury KS (eds) Wheat and Wheat Improvement (pp 388-390) American Society of Agronomy, Madison, Wisconsin
Buerstmayr H, Lemmens M, Grausgruber H and Ruckenbauer P (1996) Breeding for scab resistance in wheat: Inheritance of resistance and possibilities for in vitro selection. In: Proceedings of the workshop on Fusarium Head Scab: Global Status and Future Prospects, CIMMYT, Mexico, pp 52-58
Chen P, Liu D and Sun W (1996) New counter measures of breeding wheat for scab resistance. In: Proceedings of the workshop on Fusarium Head Scab: Global Status and Future Prospects, CIMMYT, Mexico, pp 59-65
Cook RJ (1981) Fusarium diseases of wheat and other small grains in North America. In: Nelson PE, Toussoun TA and Cook RJ (eds) Fusarium: Diseases, Biology and Taxonomy (pp 39-52) The Pennsylvania State University Press, University Park, pp 457
Deadman ML and Cooke BM (1986) A comparison of detached leaf, greenhouse and field experiments for screening barley cultivars to Drechslera teres. Irish Journal of Agricultural Research 25: 63-70
Diamond H, Cooke BM and Dunne B (1995) Microdochium Leaf Blotch of Oats — a new foliar disease caused by Microdochium nivale var. nivale in Ireland. Plant Varieties and Seeds 8: 171-174
Diamond H and Cooke BM (1997a) Host specialisation in Microdochium nivale on cereals. Cereal Research Communications 25: 533-538
Diamond H and Cooke BM (1997b) Scanning electron microscope studies on Microdochium nivale. Cereal Research Communications 25: 583-584
Dill-Macky R (1996) Fusarium head blight: Recent epidemics and research efforts in the upper midwest of the United States. Proceedings of the workshop on Fusarium Head Scab: Global Status and Future Prospects, CIMMYT, Mexico, pp 1-6
D'Mello JPF, MacDonald AM and Placinta CM (1996) Production and control of mycotoxins from Fusarium species pathogenic on cereals. In: Proceedings from the Brighton Crop Protection Conference — Pests and Diseases, pp 517-522
Eriksen GS and Alexander J (eds) (1998) Fusarium toxins in cereals — a risk assessment. Nordic Council of Ministers, Copenhagen, Denmark, Tema Nord series, pp 115
Eyal Z, Amiri Z and Wahl I (1973) Physiologic specialization of Septoria tritici. Phytopathology 63: 1087-1091
Fried PM and Meister E (1987) Inheritance of leaf and head resistance of winter wheat to Septoria nodorum in a diallel cross. Phytopathology 77: 1371-1375
Hanson EW, Ausemus ER and Stakman EC (1950) Varietal resistance of spring wheats to Fusarial head blight. Phytopathology 40: 902-914
Hömmö LM (1994) Screening winter rye cultivars for snow mould (Microdochium nivale) resistance. Plant Pathology 43: 740-750
Jalli M (1992) Ohralajikkeiden Verkkolaikunkestävyyttä Testaavat Menetelmät. University of Helsinki, Helsinki, Finland. Master's Thesis (in Finnish)
Lijuan G, Qingxiao Y, Qide H, Hao Z, Fuyou D, Huaquan Z and Wufang H (1991) Studies on screening the resistant mutants of wheat to Fusarium graminearum by tissue culture. Genetic manipulations of Plants 7: 25-33
Mesterhazy A (1987) Selection of headblight resistant wheats through improved seedling resistance. Plant Breeding 98: 25-36
Niks RE and Skinnes H (1998) Partial resistance. In: Cooke BM (ed) Airborne Pathogens on Cereals (pp 45) Office for Official Publications of the European Communities, Luxembourg, EUR 18421
Parry DW, Jenkinson P and McLeod L (1995) Fusarium ear blight (scab) in small grain cereals — a review. Plant Pathology 44: 207-238
Roderick HW and Clifford BC (1995) Variation in adult plant resistance to powdery mildew in spring oats under field and laboratory conditions. Plant Pathology 44: 366-373
Scott PR and Benedikz PW (1986) Septoria and Fusarium ear blight. Annual Report of the Plant Breeding Institute 1985 (p. 100) Plant Breeding Institute Publication, Cambridge
Snijders CHA (1990) Genetic variation for resistance to Fusarium head blight in bread wheat. Euphytica 50: 171-179
Snijders CHA and Perkowski J (1990) Effects of head blight caused by Fusarium culmorum on toxin content and weight of wheat kernels. Phytopathology 80: 566-570
Snijders CHA and Krechting CF (1992) Inhibition of deoxynivalenol translocation and fungal colonisation in Fusarium head blight resistant wheat. Canadian Journal of Botany 70: 1570-1576
Sutton JC (1982) Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum. Canadian Journal of Plant Pathology 4: 195-209
Tuite J, Shaner G and Everson RJ (1990) Wheat scab in soft red winter wheat in Indiana in 1986 and its relation to some quality measurements. Plant Disease 74: 959-962
Tusa C, Munteanu I, Capetti E, Pirvu T, Bunescu S, Sin GL, Nicolae H, Tianu A, Caea D, Romascanu O and Stoika V (1981) Aspects of the Fusarium attacks on wheat in Romania. Probleme de Protectia Plantelor 9: 15-31
Wakulinski W (1989) Phytotoxicity of the secondary metabolites of fungi causing wheat head fusariosis (head blight). Acta Physiologiae Plantarum 11: 301-306
Wang YZ and Miller JD (1988) Effects of metabolites on wheat tissue in relation to Fusarium head blight resistance. Journal of Phytopathology 122: 118-125
Wilcoxson RD, Busch RH and Ozmon EA (1992) Fusarium head blight resistance in spring wheat cultivars. Plant Disease 76: 658-661
Wong LSL, Abramson D, Tekauz A, Leisle D and McKenzie RIH (1995) Pathogenicity and mycotoxin production of Fusarium species causing head blight in wheat cultivars varying in resistance. Canadian Journal of Plant Science 75: 261-267
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Diamond, H., Cooke, B. Towards the Development of a Novel In Vitro Strategy for Early Screening of Fusarium Ear Blight Resistance in Adult Winter Wheat Plants. European Journal of Plant Pathology 105, 363–372 (1999). https://doi.org/10.1023/A:1008717602202
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DOI: https://doi.org/10.1023/A:1008717602202