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Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously

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Abstract

In order to measure residual dipolar couplings, the molecule under study has to be partially oriented in the presence of the magnetic field. It has been observed that some protein samples are not stable under the conditions imposed by the orienting media. If different types of dipolar couplings are measured sequentially, their values will not agree with a unique alignment tensor that is changing slowly over time. This could bias the structure calculation. It would be more appropriate to obtain different types of dipolar couplings simultaneously, such that all the data correspond to one effective alignment tensor. We describe here a general NMR strategy designed to do so, that can be adapted to various existing pulse sequences.

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References

  • Clore, M.G., Starich, M.R. and Gronenborn, A.M. (1998) J. Am. Chem. Soc., 120, 10571–10572.

    Google Scholar 

  • Hansen, M.R., Mueller, L. and Pardi, A. (1998) Nat. Struct. Biol., 5, 1065–1074.

    Google Scholar 

  • Kay, L.E., Xu, G.Y. and Yamazaki, T. (1994) J. Magn. Reson., 109, 129–133.

    Google Scholar 

  • Oltvai, Z.N., Milliman, C.L. and Korsmeyer, S.J. (1993) Cell, 74, 609–619.

    Google Scholar 

  • Ottiger, M., Delaglio, F. and Bax, A. (1998a) J. Magn. Reson., 131, 373–378.

    Google Scholar 

  • Ottiger, M., Delaglio, F., Marquardt, J.L., Tjandra, N. and Bax, A. (1998b) J. Magn. Reson., 134, 365–369.

    Google Scholar 

  • Ottiger, M. and Bax, A. (1999) J. Biomol. NMR, 13, 187–191.

    Google Scholar 

  • Tjandra, N. and Bax, A. (1997a) Science, 278, 1111–1114.

    Google Scholar 

  • Tjandra, N. and Bax, A. (1997b) J. Am. Chem. Soc., 119, 9576–9577.

    Google Scholar 

  • Tolman, J.R., Flanagan, J.M., Kennedy, M.A. and Prestegard, J.H. (1995) Proc. Natl. Acad. Sci USA, 92, 9279–9283.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 3, Bethesda, MD, 20892-0380, U.S.A

    Eva de Alba, Motoshi Suzuki & Nico Tjandra

Authors
  1. Eva de Alba
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  2. Motoshi Suzuki
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  3. Nico Tjandra
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de Alba, E., Suzuki, M. & Tjandra, N. Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously. J Biomol NMR 19, 63–67 (2001). https://doi.org/10.1023/A:1008341128327

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  • DOI: https://doi.org/10.1023/A:1008341128327

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