Abstract
Solvent exchange rates and temperature coefficients for Asn/Gln side-chain amide protons have been measured in Escherichia coli HPr. The protons of the eight side-chain amide groups (two Asn and six Gln) exhibit varying exchange rates which are slower than some of the fast exchanging backbone amide protons. Differences in exchange rates of the E and Z protons of the same side-chain amide group are obtained by measuring exchange rates at pH values > 8. An NOE between a side-chain amide proton and a bound water molecule was also observed.
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Bai, Y., Milne, J. S., Mayne, L. and Englander, S. W. (1993) Proteins Struct. Funct. Genet., 17, 75–86.
Delaglio, F., Grzesiek, S., Vuister, G., Zhu, G., Pfeifer, J. and Bax, A. (1995) J. Biomol. NMR, 6, 277–293.
Baxter, N. J. and Williamson, M. P. (1997) J. Biomol. NMR, 9, 359–369.
Farrow, N. A., Muhandiram, R., Singer, A. U., Pascal, S. M., Kay, C. M., Gish, G., Shoelson, S. E., Pawson, T., Forman-Kay, J. D. and Kay, L. E. (1994) Biochemistry, 33, 5984–6003.
Garrett, D. S., Powers, R., Gronenborn, A. N. and Clore, G. M. (1991) J. Magn. Reson., 95, 214–220.
Grzesiek, S. and Bax, A. (1993a) J. Am. Chem. Soc., 115, 12593–12594.
Grzesiek, S. and Bax, A. (1993b) J. Biomol. NMR, 3, 627–638.
Hammen, P. K., Waygood, E. B. and Klevit, R. E. (1991) Biochemistry, 30, 11842–11850.
Jia, Z., Van Donselaer, M., Hengstenberg, W., Quail, J. W. and Delbaere, L. T. (1994) J. Mol. Biol., 236, 1341–1355.
Krishna, N. R., Sarathy, K. P., Huang, D. H., Stephens, R. L., Glickson, J. D., Smith, C. W. and Walter, R. (1982) J. Am. Chem. Soc., 104, 5051–5053.
Lee, B. and Richards, F. M. (1971) J. Mol. Biol., 55, 379–400.
Löhr, F. and Rüterjans, H. (1997) J. Magn. Reson., 124, 255–258.
Narutis, V. P. and Kopple, K. D. (1983) Biochemistry, 22, 6233–6239.
O'Neil, J. D. J. and Sykes, B. D. (1989) Biochemistry, 28, 6736–6745.
Peng, J. W. and Wagner, G. (1992) J. Magn. Reson., 98, 308–332.
Rajagopal, P. and Klevit, R. E. (1994) Tech. Protein Chem., 5, 439–445.
Redfield, A. G. and Waelder, S. (1979) J. Am. Chem. Soc., 101, 6151–6162.
Spera, S., Ikura, M. and Bax, A. (1991) J. Biomol. NMR, 1, 155–165.
Tüchsen, E. and Woodward, C. (1987) Biochemistry, 26, 8073–8078.
van Nuland, N. A. J., Grotzinger, J., Dijkstra, K., Scheek, R. M. and Robillard, G. T. (1992) Eur. J. Biochem., 210, 881–891.
Wüthrich, K. and Wagner, G. (1979) J. Mol. Biol., 130, 1–18.
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Rajagopal, P., Jones, B.E. & Klevit, R.E. Solvent Exchange Rates of Side-chain Amide Protons in Proteins. J Biomol NMR 11, 205–212 (1998). https://doi.org/10.1023/A:1008296932751
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DOI: https://doi.org/10.1023/A:1008296932751