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ATPase and ubiquitin-binding proteins of the yeast proteasome

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Abstract

The 26S proteasome is a 2-Megadalton proteolytic complex with over 30 distinct subunits. The 19S particle, a subcomplex of the 26S proteasome, is thought to confer ATP-dependence and ubiquitin-dependence on the proteolytic core particle of the proteasome. Given the complexity of the 19S particle, genetic approaches are likely to play an important role in its analysis. We have initiated biochemical and genetic studies of the 19S particle in Saccharomyces cerevisiae. Here we describe the localization to the proteasome of several ATPases that were previously proposed to be involved in transcription. Independent studies indicate that the mammalian 26S proteasome contains closely related ATPases. We have also found that the multiubiquitin chain binding protein Mcb1, a homolog of the mammalian S5a protein, is a subunit of the yeast proteasome. However, contrary to expectation, MCB1 is not an essential gene in yeast. The mcb1 mutant grows at a nearly wild-type rate, and the breakdown of most ubiquitin-protein conjugates is unaffected in this strain. One substrate, Ub-Proline-βgal, was found to require MCB1 for its breakdown, but it remains unclear whether Mcb1 serves as a ubiquitin receptor in this process. Our data suggest that the recognition of ubiquitin conjugates by the proteasome is a complex process which must involve proteins other than Mcb1.

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Authors and Affiliations

  1. Department of Cell Biology, Harvard Medical School, 40 Longwood Ave., Boston, MA, 02146, USA

    David M. Rubin, Michael Glickman, Olivier Coux, Inge Wefes, Seth Sadis, Alfred Goldberg & Daniel Finley*

  2. Department of Horticulture, University of Wisconsin, Madison, WI, 53706, USA

    Steve van Nocker, Hongyong Fu & Richard Vierstra

Authors
  1. David M. Rubin
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  2. Steve van Nocker
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  3. Michael Glickman
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  4. Olivier Coux
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  5. Inge Wefes
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  6. Seth Sadis
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  7. Hongyong Fu
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  8. Alfred Goldberg
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  9. Richard Vierstra
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  10. Daniel Finley*
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Rubin, D.M., van Nocker, S., Glickman, M. et al. ATPase and ubiquitin-binding proteins of the yeast proteasome. Mol Biol Rep 24, 17–26 (1997). https://doi.org/10.1023/A:1006844305067

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