Abstract
Inflammatory cytokines, such as interleukin (IL)-6, have been shown to reflect clinical signs in certain conditions in diseased animals. In this study, we quantified the IL-6 concentrations in the serum and milk whey from 94 dairy cows with acute clinical mastitis and 55 healthy lactating cows. The IL-6 concentrations in serum from mastitic cows were significantly higher on the first day of illness compared to those of normal cows. Higher concentrations of IL-6 were also detected in the whey from mastitic cows, whereas low concentrations of IL-6 were detected in both serum and whey samples from normal cows. IL-6 concentrations in the serum taken at the onset of illness from cows that later required euthanasia were significantly higher than those in samples from cows that later recovered. These results suggest that serum IL-6 concentrations may be of prognostic value in identifying cows with severe mastitis.
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REFERENCES
Barber, M.R. and Yang, T.J., 1998. Chemotactic activities in nonmastitic and mastitic mammary secretions: presence of interleukin-8 in mastitic but not nonmastitic secretions. Clinical and Diagnostic Laboratory Immunology, 5, 82-86
Björk, L., Anderson, J., Ceska, M. and Andersson, U., 1992. Endotoxin and Staphylococcus aureus enterotoxin A induce different patterns of cytokines. Cytokine, 4, 513-519
Colditz, I. G., 1985. Margination and emigration of leukocytes. Survey of Synthetic Pathogen Research, 4, 44-68
Cybulsky, M.I., McCombo, D.J. and Hovat, H.Z., 1988. Neutrophil leukocyte emigration induced by endotoxin. Mediator roles of interleukin 1 and tumor necrosis factor. Journal of Immunology, 140, 3144-3149
Daley, M.J., Oldham, E.R., Williams, T.J. and Coyle, P.A., 1991. Bovine mastitis. I. Quantitative and qualitative properties of host polymorphonuclear cells during Staphylococcus aureus mastitis infection. American Journal of Veterinary Research, 52, 474-479
Degraves, F.J. and Fetrow, J., 1993. Economics of mastitis and mastitis control.Veterinary Clinics of North America, Food Animal Practice, 9, 421-434
Eberhart, R.J., Natzke, R.P., Newbould, F.H.S., Nonneck, B. and Thompson, P., 1979. Coliform mastitis–a review. Journal of Dairy Science, 62, 1-22
Fassebender, K., Gerber, B., Karrer, U., Sobieska, M., Aeschlimann, A. and Müller, W., 1993. Glycosylation of acute-phase proteins and interleukins following total hip arthroplasty. Acta Orthopaedica Scandinavica, 64, 212-215
Fassebender, K., Dempfle, C.E., Mielke, O., Rossol, S., Schneider, S., Dollman, M. and Hennerici, M., 1997. Proinflammatory cytokines: indicators of infection in high-risk patients. Journal of Laboratory Clinical Medicine, 130, 535-539
Godson, D.L., Baca-Estrada, M.E., Van Kessel, A.G., Hughes, H.P., Morsy, M.A., Van Donkersgoed, J., Harland, R.J., Shuster, D.E., Daley, M.J. and Babiuk, L.A., 1995. Regulation of bovine acute-phase responses by recombinant interleukin-1 beta. Canadian Journal of Veterinary Research, 59, 249-255
Goto, M., Maruyama, M., Kitadate, K., Kirisawa, R., Obata, Y., Koiwa, M. and Iwai, H., 1997. Detection of interleukin-1b in sera and colostrum of dairy cattle and in sera of neonates. Journal of Veterinary Medical Science, 59, 437-441
Hoblet, K.H., Schnitkey, G.D., Arbaugh, D., Hogan, J.S., Smith, K.L., Schoenberger, P.S., Todhunter, D.A., Hueston, W.D., Pritchard, D.E., Bowman, G.L., Heider, L.E., Brockett, B.L. and Conrad, H.R., 1991. Cost associated with selected preventive practices and with episodes of clinical mastitis in nine herds with low somatic cell counts. Journal of the American Veterinary Medical Association, 199, 190
Miller, G.Y., Bartlett, P.C., Lance, S.E., Anderson, J.D. and Heider, L.E., 1993. Cost of clinical mastitis and mastitis prevention in dairy herds. Journal of the American Veterinary Medical Association, 202, 1230
Nakajima, Y., Momotani, E., Murakami, R., Ishikawa, Y., Morimatsu, M., Saito, M., Suzuki, H. and Yasukawa, K., 1993. Induction of acute-phase protein by recombinant human interleukin-6 (IL-6) in calves.Veterinary Immunology and Immunopathology, 35, 385-391
Persson, K., Colditz, I.G., Flapper, P., Franklin, N.A. and Seow, H.-F., 1996. Cytokine-induced inflammation in the ovine teat and udder. Veterinary Immunology and Immunopathology, 53, 73-85
Persson, K., Colditz, I.G. and Seow, H.F., 1997. Accumulation of leucocytes and cytokines in the lactating ovine udder during mastitis due to Staphylococcus aureus and Escherichia coli. Research in Veterinary Science, 62, 63-66
Philpot, W.N. and Nickerson, S.C., 1991. Mastitis: Counterattack, (Bobson Bros., Co., Naperville, Illinois), 1-133
Schalm, O.W. and Noorlander, D.O., 1957. Experiments and observations leading to development of California Mastitis Test. Journal of the American Veterinary Medical Association, 130, 199-204
Shuster, D.E. and Kehrli, M.E., 1993. Cytokine production during endotoxin-induced mastitis in lactating dairy cows. American Journal of Veterinary Research, 54, 80-85
Shuster, D.E., Lee, E.K. and Kehrli, M.E., 1996. Bacterial growth, inflammatory cytokine production, and neutrophil recruitment during coliform mastitis in cows within ten days after calving, compared with cows at midlactation. American Journal of Veterinary Research, 57, 1569-1575
Sordillo, L.M., Redmond, M.J., Campos, M., Warren, L. and Babiuk, A., 1991. Cytokine activity in bovine mammary gland secretions during the periparturient period. Canadian Journal of Veterinary Research, 55, 298-301
Taylor, B.C., Keefe, R.G., Dellinger, J.D., Nakamura, Y., Cullor, J.S. and Stott, J.L., 1997. T cell population and cytokine expression in milk derived from normal and bacteria-infected bovine mammary glands. Cellular Immunology, 182, 68-76
Yoshino, K., Katoh, N., Takahashi, K. and Yuasa, A., 1993. Possible involvement of protein kinase C with induction of haptoglobin in cows by treatment with dexamethasone and by starvation. American Journal of Veterinary Research, 54, 689-694
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Hagiwara, K., Yamanaka, H., Hisaeda, K. et al. Concentrations of IL-6 in Serum and Whey from Healthy and Mastitic Cows. Vet Res Commun 25, 99–108 (2001). https://doi.org/10.1023/A:1006400801305
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DOI: https://doi.org/10.1023/A:1006400801305