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In vitro proliferation of shoots and regeneration of cotton

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Abstract

Shoot proliferation from different explants of several Indian cultivars of cotton was studied in culture. Cotyledonary nodes taken along with the shoot apex of seedlings produced multiple shoots on modified MS nutrient agar supplemented with cytokinins. 6-Benzyladenine was most effective in inducing growth of multiple shoots. Explants of several genotypes formed organogenic masses that differentiated to secondary shoots on repeated subculture. The isolated shoots were rooted on basal medium supplemented with naphthaleneacetic acid and were transferred to soil after acclimatization.

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Authors and Affiliations

  1. National Botanical Research Institute, Rana Pratap Marg, Lucknow-, 226 001, India

    Shiv K. Gupta, Alok K. Srivastava, Pradhyumna K. Singh & Rakesh Tuli

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  1. Shiv K. Gupta
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  2. Alok K. Srivastava
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  3. Pradhyumna K. Singh
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  4. Rakesh Tuli
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Gupta, S.K., Srivastava, A.K., Singh, P.K. et al. In vitro proliferation of shoots and regeneration of cotton. Plant Cell, Tissue and Organ Culture 51, 149–152 (1997). https://doi.org/10.1023/A:1005906810364

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  • DOI: https://doi.org/10.1023/A:1005906810364

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