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Effects of low temperature on induction and differentiation of wheat calluses

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Abstract

Young inflorescences and long-term culture calluses of wheat (Triticum aestivum L.) were used to investigate the effect of low temperature treatment on the potential of plant regeneration. The results indicated that the frequency of callus induction from immature inflorescences was decreased when treated for a long time at 5 °C. However, it was found that a 5 °C treatment significantly improved the regeneration frequency of calluses. The clear difference of peroxidases of wheat calluses was apparent after the cultures were treated at the low temperature. The isozyme band 8 became clearly faint, however, bands 6 and 9 became intense.

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Authors and Affiliations

  1. Department of Biology, Shandong University, Jinan, Shandong, 250100, P. R. China

    Bingkai Hou & Shiyun Teng

  2. Department of Biology, Shandong Education College, Jinan, Shandong, 250013, P. R. China

    Huimin Yu

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  1. Bingkai Hou
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  2. Huimin Yu
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  3. Shiyun Teng
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Hou, B., Yu, H. & Teng, S. Effects of low temperature on induction and differentiation of wheat calluses. Plant Cell, Tissue and Organ Culture 49, 35–38 (1997). https://doi.org/10.1023/A:1005854301398

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  • DOI: https://doi.org/10.1023/A:1005854301398

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