Abstract
A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5′-flanking sequence contains heat shock elements and other potential regulatory sequences.
References
Aly R, Argaman M, Halman S, Shapira M: Aregulatory role for the 5′and 3′ untranslated regions in differential expression of hsp83 in Leishmania. Nucl Acids Res 22: 2922–2929 (1994).
Amir-Shapira D, Leustek T, Dalie B, Weissbech H, Brot N: HSP70 proteins, similar to Escherichia coli DnaK, in chloroplasts and mitochondria of Euglena gracilis. Proc Natl Acad Sci USA 87: 1749–52 (1990).
Boorstein WR, Ziegelhoffer T, Craig EA: Molecular evolution of the hsp70 multigene family. J Mol Evol 38: 1–17 (1994).
Chirico WJ, Waters MG, Blobel G: 70k heat shock related proteins stimulate protein translocation into micosomes. Nature 332: 805–810 (1988).
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction. Anal Biochem 162: 156–159 (1987).
Craig EA, Krammer J, Shilling J, Werner-Washbourne M, Holmes S, Kosics-Smithers J, Nicolet CM: SSC1, an essential member of the S. cerevisiae HSP70 multigene family, encodes a mitochondrial protein. Mol Cell Biol 9: 3000–3008 (1989).
DeRocher AE, Vierling E: Cytoplasmic hsp70 homologues of pea: differential expression in vegetative and embryonic organs. Plant Mol Biol 27: 441–456 (1995).
Drew JE, Gatehouse JA: Isolation and characterization of a pea pod cDNA encoding a putative blue copper protein correlated with lignin deposition. J Exp Bot 45: 1873–1884 (1994).
Duck N, McCormick S, Winter J: Heat shock protein hsp70 cognate gene expression in vegetative and reproductive organs of Lycopersicon esculentum. Proc Natl Acad Sci USA 86: 3674–3678 (1989).
Edington BV, Whelan SA, Hightower LE: Inhibition of heat shock stress/protein induction by deuterium oxide and glycerol: additional support for the abnormal protein hypothesis of induction. J Cell Physiol 139: 219–228 (1989).
Fernandez M, O'Brien T, Lis JT: Structure and regulation of heat shock gene promoters. In: Morimoto RI, Tissieres A, Georgopolous C (eds) The Biology of Heat Shock Proteins and Molecular Chaperones, pp. 375–393. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1994).
Gaurley WB, Czarnecka E, Barros MDC: Anatomy of soybean heat shock promoter. In: DPS Verma (ed) Control of Plant Gene Expression, pp. 103–123. CRC Press, Boca Raton, FL (1993).
Graham DE: The isolation of high molecular weight DNA from whole organisms or large tissue masses. Anal Biochem 85: 609–613 (1977).
Handrick JP, Hartl FU: Molecular chaperone functions of heat shock proteins. Annu Rev Biochem 62: 349–384 (1993).
Heikkila JJ, Papp JET, Schultz GA, Bewley JD: Induction of heat shock protein mRNA in maize mesocotyls by water stress, abscisic acid and wounding. Plant Physiol 76: 270–274 (1984).
Lin T-Y, Duck NB, Winter J, Folk WR: Sequences of two hsc70 cDNAs from Lycopersicon esculentum. Plant Mol Biol 16: 47–78 (1991).
Lindquist S: The heat shock response. Annu Rev Biochem 55: 1151–1191 (1986).
Lindquist S, Craig EA: The heat shock proteins. Annu Rev Genet 22: 631–677 (1988).
Marshall JS, DeRocher AE, Keegstra K, Vierling E: Identification of heat shock protein hsp70 homologues in chloroplasts. Proc Natl Acad Sci USA 87: 374–378 (1990).
Morimoto RJ, Tissieres A, Georgopoulos C: In: The stress response, functions of the proteins and perspectives. pp. 1–36. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1990).
Munro S, Pelham HRB: An HSP-like protein in the ER: identify with the 78 kD glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46: 291–300 (1986).
Neven LG, Haskell DW, Guy CL, Denslow N, Klein PA, Green LG, Silverman A: Association of 70 kD heat-shock cognate proteins with acclimatisation to cold. Plant Physiol 99: 1362–1369 (1992).
Neumann D, Nover L, Parthier B, Rieger R, Scharf K-D, Wollgiehn R, Nieden UZ: Heat shock and other stress response system in plants. Biol Zentralbl 108: 1–156 (1989).
Olszeski NE, Gast RT, Ausubell FM: A dual labelling method for identifying differentially expressed genes used in the identification of cDNA clones that hybridize to mRNAs whose abundance in tomato flowers is potentially regulated by gibberelins. Gene 77: 155–162 (1989).
Pelham HRB: Speculations on the function of the major heat shock and glucose-regulated proteins. Cell 46: 959–961 (1986).
Roberts JM, Key JL: Isolation and characterisation of a soyabean hsp70 gene. Plant Mol Biol 16: 671–683 (1991).
Rochester D, Winter JA, Shah DM: The structure and expression of maize genes encoding the major heat shock protein hsp70. EMBO J 5: 451–458 (1986).
Vierling E: The roles of heat shock proteins in plants. Annu Rev Plant Physiol Mol Biol 42: 579–620 (1991).
Voellmy R: The heat shock genes: a family of highly conserved genes with a superbly complex expression pattern. Bioessays 1: 213–217 (1984).
Welch WJ, Mizzan L: Characterisation of the thermotolerant cell. II. Effects on the intercellular distribution of the heatshock protein 70, intermediate filaments and small nuclear ribonucleoprotein complexes. J Cell Biol 106: 1117–1130 (1988).
Winter J, Wright R, Duck N, Gasser C, Fraley R, Shah D: The inhibition of petunia hsp70 mRNA processing during CdCl2 stress. Mol Gen Genet 211: 315–319 (1988).
Wu CH, Caspar T, Browse J, Lindquist S, Somerville C: Characterization of an hsp70 cognate gene family in Arabidopsis. Plant Physiol 88: 731–748 (1988).
Zimmerman JL, Apuya N, Darwish K, 'Carroll C: Novel regulation of heat shock genes during carrot somatic embryo development. Plant Cell 1: 1137–1146 (1989).
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Parkash Dhankher, O., Drew, J.E. & Gatehouse, J.A. Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated. Plant Mol Biol 34, 345–352 (1997). https://doi.org/10.1023/A:1005804612280
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DOI: https://doi.org/10.1023/A:1005804612280