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Production of inulooligosaccharides from inulin by a novel endoinulinase from Xanthomonas sp.

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Abstract

A novel inulinolytic microorganism, Xanthomonas sp. produced an endoinulinase, to be used for inulooligosaccharide (IOS) formation from inulin, at an activity of 11 units ml−1 (1.2 mg protein ml−1). The endoinulinase was optimally active at 45 °C and pH 6.0. Batchwise production of IOS was carried out by the partially purified endoinulinase with a maximum yield of about 86% on a total sugar basis with 10 g inulin l−1. The major IOS components were DP (degree of polymerization) 5 and 6 with trace amount of smaller oligosaccharides.

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Authors and Affiliations

  1. Department of Biotechnology, Taegu University, Kyungbuk, 712–714, Korea

    J.P. Park, J.T. Bae, B.W. Kim & J.W. Yun

  2. Department of Microbiology, Dongeui University, Pusan, 614-714, Korea

    D.J. You

Authors
  1. J.P. Park
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  2. J.T. Bae
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  3. D.J. You
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  4. B.W. Kim
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  5. J.W. Yun
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Park, J., Bae, J., You, D. et al. Production of inulooligosaccharides from inulin by a novel endoinulinase from Xanthomonas sp.. Biotechnology Letters 21, 1043–1046 (1999). https://doi.org/10.1023/A:1005632526442

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  • DOI: https://doi.org/10.1023/A:1005632526442

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