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Effects of Salinity on Endogenous Aba, Iaa, Ja, and Sa in Iris hexagona

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Abstract

Phytohormones play critical roles in regulating plant responses to stress. We investigated the effects of salinity on abscisic acid (ABA), indole-3-acetic acid (IAA), salicylic acid (SA), and jasmonic acid (JA) in leaves, stalks, fruits, and seeds of Iris hexagona, a native wetland species. Using gas chromatography–mass spectroscopy with selected ion monitoring, our experiments demonstrated significant and different shortand long-term changes in iris phytohormones. ABA and JA generally increased and IAA and SA declined in response to salinity. We conclude that these phytohormones may have separate and interactive effects on how plants respond and adapt to stress in natural environments.

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REFERENCES

  • ABBAS, M. A., YOUNIS, M. E., and SHUKRY, W. M. 1991. Plant growth, metabolism and adaptation in relation to stress conditions. 14. Effect of salinity on the internal solute concentrations in Phaseolus vulgaris. J. Plant Physiol.138:722-727.

    Google Scholar 

  • AGRAWAL, A. A., STRAUS, S. Y., and SOUT, M. J. 1999. Costs of induced responses and tolerance to herbivory in male and female fitness components of wild radish. Evolution53:1093-1104.

    Google Scholar 

  • AMZALLAG, G. N., LERNER, H. R., and POLJAKOFF-MAYBER, A. 1990. Exogenous ABA as a modulator of the response of sorghum to high salinity. J. Exp. Bot.41:1529-1394.

    Google Scholar 

  • BALDWIN, I. T. 1994. Wound-induced changes in root and shoot jasmonic acid pools correlate with induced nicotine synthesis in Nicotiana sylvestrisspegazzi and comes. J. Chem. Ecol.20:2139-2157.

    Google Scholar 

  • BASKIN, C. C., and BASKIN, J. M. 1998. Seeds: Ecology, Biogeography, and Evolution of dormancy and germination. Academic Press, New York pp. 41-43.

    Google Scholar 

  • BERGEY, D. R., HOWE, G. A., and RYAN, C. A. 1996. Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals. Proc. Natl. Acad. Sci. U.S.A.93:12053-12058.

    PubMed  Google Scholar 

  • BLECHERT, S., BRODSCHELM, W., HOLDER, S., KAMMERER, L., KUTCHAN, T. M., MUELLER, M. J., XIA, Z. H., and ZENK, M. H. 1995. The octadecanoic pathway: Signal molecules for the regulation of secondary pathways. Proc. Natl. Acad. Sci. U.S.A.92:4099-4105.

    PubMed  Google Scholar 

  • BRUGNOLI, E., and BJORKMAN, O. 1992. Growth of cotton under continuous salinity stress-influence on allocation pattern, stomatal, and nonstomatal components of photosynthesis and dissipation of excess light energy. Planta187:335-347.

    Article  Google Scholar 

  • CHADHA, K. C., and BROWN, S. A. 1974. Biosynthesis of phenolic acids in tomato infected with Agrobacterium tumefaciens. Can. J. Bot.52:2041-2046.

    Google Scholar 

  • CHAO, W. S., GU, Y. Q., PAUTOT, V., BRAY, E. A., and WALLING, L. L. 1999. Leucine aminopeptidase RNAs, proteins, and activities increase in response to water deficit, salinity, and the wound signals systemin, methyl jasmonate, and abscisic acid. Plant Physiol.120:979-992.

    PubMed  Google Scholar 

  • CLELAND, F. C., and AJAMI, A. 1974. Identification of the flower inducing factor isolated from aphid honeydew as being salicylic acid. Plant Physiol.54:904-906.

    Google Scholar 

  • CLIPSON, N. J. W., LACHNO, D. R., and FLOWERS, T. J. 1988. Salt tolerance in the halophyte Suaeda maritimaL. Dum: abscisic acid concentrations in response to constant and altered salinity. J. Exp. Bot.39:1381-1388.

    Google Scholar 

  • CREELMAN, R. A., and MULLET, J. E. 1995. Jasmonic acid distribution and action in plants: Regulation during development and in response to biotic and abiotic stress. Proc. Natl. Acad. Sci. U.S.A.92:4114-4119.

    PubMed  Google Scholar 

  • CREELMAN, R. A., and MULLET, J. E. 1997. Biosynthesis and action of jasmonates in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.48:355-381.

    Google Scholar 

  • CREELMAN, R. A., TIERNEY, M. L., and MULLET, J. E. 1992. Jasmonic acid methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc. Natl. Acad. Sci. U.S.A.89:4938-4941.

    PubMed  Google Scholar 

  • CURTIS, R. W. 1984. Abscision-inducing properties of methyl jasmonates and, ABA, and ABA-methyl ester and their interactions with ethephone and AgNO3. J. Plant Growth Regul.3:157-163.

    Google Scholar 

  • DELESALLE, V. A., and MAZER, S. J. 1996. Nutrient levels and salinity affect gender and floral traits in the autogamous Spergularia marian. J. Plant Sci.157:621-631.

    Google Scholar 

  • DOARES, S. H., NARVAEZ-VASQUEZ, J., CONCONI, A., and RYAN, C. A. 1995. Salicylic acid inhibits synthesis of proteinase inhibitors in tomato leaves induced by systemin and jasmonic acid. Plant Physiol.108:1741-1746.

    PubMed  Google Scholar 

  • DUNLAP, J. R., and BINZEL, M. L. 1996. NaCl reduces indole-3-acetic acid levels in the roots of tomato plants independent of stress-induced abscisic acid. Plant Physiol.112:379-384.

    PubMed  Google Scholar 

  • DUNN, D. C., DUNCAN, L. W., and ROMEO, J. T. 1998. Changes in arginine, PAL activity, and nematode behavior in salinity-stressed citrus. Phytochemistry49:413-417.

    PubMed  Google Scholar 

  • EBERHARDT, H. J., and WEGMAN, K. 1989. Effects of abscisic acid and proline on adaptation of tobacco callus culture to salinity and osmotic shock. Physiol. Plant.76:283-288.

    Google Scholar 

  • EL-ENANY, A. E. 2000. Abscisic acid-responsive proteins induce salinity tolerance in wheat seedlings. Acta Physiol. Plant.22:53-59.

    Google Scholar 

  • ENYEDI, A. J., YALPNI, N., SILVERMAN, P., and RASKIN, I. 1992. Signal molecules in systemic plant resistance to pathogens and pests. Cell70:879-886.

    Article  PubMed  Google Scholar 

  • FARMER, E. E., JOHNSON, R. R., and RYAN, C. A. 1992. Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid. Plant Physiol.98:995-1002.

    Google Scholar 

  • FINCH-SAVAGE, W. E., BLAKE, P. S., and CLAY, H. A. 1996. Desiccation stress in recalcitrant Quercus roburL. seeds results in lipid peroxidation and increased synthesis of jasmonates and abscisic acid. J. Exp. Bot.47:661-667.

    Google Scholar 

  • GLINKA, Z., and REINHOLD, L. 1971. Abscisic acid raises the permeability of plant cells to water. Plant Physiol.48:103-105.

    Google Scholar 

  • GUPTA, V., WILLITS, M. G., and GLAZEBROOK, J. 2000. Arabidopsis thalianaEDS4 contributes to salicylic acid (SA)-dependent expression of defense responses: Evidence for inhibition of jasmonic acid signaling by SA. Mol. Plant-Microbe Interact.13:503-511.

    PubMed  Google Scholar 

  • HARMS, K., RAMIREZ, I., and PENACORTES, H. 1998. Inhibition of wound-induced accumulation of allene oxide synthase transcripts in flax leaves by aspirin and salicylic acid. Plant Physiol.118:1057-1065.

    PubMed  Google Scholar 

  • HOYOS, M. E., and ZHANG, S. Q. 2000. Calcium-independent activation of salicylic acid-induced protein kinase and a 40-kilodalton protein kinase by hyperosmotic stress. Plant Physiol.122:1355-1363.

    PubMed  Google Scholar 

  • IUCHI, S., YAMAGUCHI-SHINOZAKI, K., URAO, T., and SHINOZAKI, K. 1996. Characterization of two cDNAs for novel drought-inducible genes in the highly drought-tolerant cowpea. J. Plant Res.109:415-424.

    Google Scholar 

  • JIN, S., CHEN, C. C. S., and PLANT, A. L. 2000. Regulation by ABA of osmotic-stress-induced changes in protein synthesis in tomato roots. Plant Cell Environ.23:51-60.

    Google Scholar 

  • LAROSA, P. C., HANNDA, A. K., HASGAWA, P. M., and BRESSAN, R. A. 1985. Abscisic acid accelerates adaptation of cultured tobacco cells to salt. Plant Physiol.79:138-142.

    Google Scholar 

  • LEVITT, J., 1980. Responses of Plants to Environmental Stresses, Vol. II. Academic Press, New York.

    Google Scholar 

  • LOPEZ-CARBONELL, M., ALEGRE, L., PASTOR, A., PRINSEN, E., and VAN ONCKELEN, H. 1996. Variations in abscisic acid, indole-3-acetic acid and zeatin riboside concentrations in two Mediterranean shrubs subjected to water stress. Plant Growth Regul.20:271-277.

    Google Scholar 

  • MARTINEZ-TELLEZ, M. A., and LAFUENTE, M. T. 1997. Effect of high temperature conditioning on ethylene, phenylalanine ammonialyase, peroxidase and polyphenol oxidase activities in flavedo of chilled “Fortune” mandarin fruit. J. Plant Physiol.150:674-678.

    Google Scholar 

  • MCCLOUD, E. S., and BALDWIN, I. T. 1997. Herbivory and caterpillar regurgitant amplify the wound-induced increases in jasmonic acid but not nicotine in Nicotiana sylvestris. Planta203:430-435.

    Google Scholar 

  • MONTERO, E., CABOT, C., BARCELO, J., and POSCHENRIEDER, C. 1997. Endogenous abscisic acid levels are linked to decreased growth of bush bean plants treated with NaCl. Physiol. Plant.101:17-22.

    Google Scholar 

  • MOONS, A., PRISEN, E., BAUW, G., and MONTAGU, M. V. 1997. Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots. Plant Cell92:243-259.

    Google Scholar 

  • MORALES, M. A., SANCHEZ-BLANCO, M. J., OLMOS, E., TORRECILLAS, A., and ALARCON, J. J. 1998. Changes in the growth, leaf water relations and cell ultrastructure in Argyranthemum coronopifoliumplants under saline conditions. J. Plant Physiol.153:174-180.

    Google Scholar 

  • MORGAN, J. M. 1984. Osmoregulation and water-stress in higher-plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.36:299-319.

    Google Scholar 

  • NIKI, T., MITSUHARA, I., SEO, S., OHTSUBO, N., and OHASHI, Y. S. 1998. Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco leaves. Plant Cell Physiol.39:500-507.

    Google Scholar 

  • NILSEN, E. T., and ORCUTT, D. M. 1996. The physiology of Plants Under Stress-Abiotic Factors. John Wiley & Sons, New York, pp. 118-130.

    Google Scholar 

  • PASTERNAK, D. 1987. Salt tolerance and crop production-a comprehensive approach. Annu. Rev. Phytopathol.25:271-291.

    Google Scholar 

  • PENA-CORTES, H., ALBRECHT, T., PRAT, S., WEILER, E. W., and WILLMITZER, L. 1993. Aspirin prevents wound-induced gene expression in tomato leaves by blocking jasmonic acid biosynthesis. Planta191:123-128.

    Article  Google Scholar 

  • RASKIN, I. 1992. Roles of salicylic acid in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.43:439-463.

    Google Scholar 

  • RASKIN, I., SKUBATZ, H., TANG, W., and MEEUSE, B. J. D. 1990. Salicylic acid levels in thermogenic and non-thermogenic plants. Ann. Bot.66:369-373.

    Google Scholar 

  • SAS Institute. 1994. SAS STATS USERS GUIDE. Vol. 1. 4th ed. SAS Institute Inc. Cary, North Carolina.

    Google Scholar 

  • SEMBDNER, G., and PARTHIER, B. 1993. The biochemistry and physiology and molecular actions of jasmonates. Annu. Rev. Plant Physiol. Plant Mol. Biol.44:569-586.

    Google Scholar 

  • SERRANO, R., and GAXIOLA, R. 1994. Microbial models and salt stress tolerance in plants. Crit. Rev. Plant Sci.13:121-138.

    Google Scholar 

  • SHINOZAKI, K., YAMAGUCHI-SHINOZAKI, K., MIZOGUCHI, T., URAO, T., KATAGIRI, T., NAKASHIMA, K., ABE, H., ICHIMURA, K., LIU, Q. A., NANJYO, T., UNO, Y., IUCHI, S., SEKI, M., ITO, T., HIRAYAMA, T., and MIKAMI, K. 1998. Molecular responses to water stress in Arabidopsis thaliana. J. Plant Res.111:345-351.

    Google Scholar 

  • SKRIVER, K., and MUNDY, J. 1990. Gene expression and response to abscisic acid and osmotic stress. Plant Cell2:503-512.

    Article  PubMed  Google Scholar 

  • STASWICK, P. E., and LEHMAN, C. C. 1999. Jasmonic acid-signaled responses in plants, pp. 117-150, inA. Agrawal, S. Tuzun, and E. Bent (eds.). Induced Plant Defenses Against Pathogens and Herbivores: Biochemistry, Ecology, and Agriculture. APS Press, St. Paul, Minnesota.

    Google Scholar 

  • SWAIN, A. R., DUTTON, S. P., and TRUSWELL, A. S. 1985. Salicylates in foods. J. Am. Diet. Assoc.85:950-960.

    PubMed  Google Scholar 

  • TALANOVA, V. V., and TITOV, A. F. 1994. Endogenous abscisic acid content in cucumber leaves under the influence of unfavorable temperature and salinity. J. Exp. Bot.45:1031-1033.

    Google Scholar 

  • THALER, J. S. 1999. Jasmonate-inducible plant defenses cause increased parasitism of herbivores. Nature399:686-688.

    Google Scholar 

  • THALER, J. S., STOUT, M. J., KARBAN, R., and DUFFEY, S. S. 1996. Exogenous jasmonates simulate insect wounding in tomato plants (Lycopersicon esculentum) in the laboratory and field. J. Chem. Ecol.22:1767-1781.

    Google Scholar 

  • THALER, J. S., FIDANTSEF, A. L., DUFFEY, S. S. and BOSTOCK, R. M. 1999. Trade-offs in plant defense against pathogens and herbivores: A field demonstration of chemical elicitors of induced resistance. J. Chem. Ecol.25:1597-1609.

    Google Scholar 

  • THOMAS, J. C., and BOHNERT, H. J. 1993. Salt stress perception and plant growth regulators in the halophyte Mesembryanthemum crystallinum. Plant Physiol.103:1299-1304.

    PubMed  Google Scholar 

  • THOMASHOW, M. F. 1999. Plant cold acclimation: Freezing tolerance genes and regulatory mechanisms Annu. Rev. Plant Physiol. Plant Mol. Biol.50:571-599.

    PubMed  Google Scholar 

  • TOMAS-BARBERAN, F. A., LOAIZAVELARDE, J., BONFANTI, A., and SALTVEIT, M. E. 1997. Early wound and ethylene-induced changes in phenylpropanoid metabolism in harvested lettuce. J. Am. Soc. Hortic. Sci.122:399-404.

    Google Scholar 

  • TSONEV, T. D., LAZOVA, G. N., STOINOVA, Z. G., and POPOVA, L. P. 1998. A possible role for jasmonic acid in adaptation of barley seedlings to salinity stress. J. Plant Growth Regul.17:153-159.

    Google Scholar 

  • YALPANI, N., SHULAEV, V., and RASKIN, I. 1993. Endogenous salicylic acid levels correlate with accumulation of pathogenesis-related proteins and virus resistance in tobacco. Phytopathology83:702-708.

    Google Scholar 

  • ZENG, X. C., ZHOU, X., ZHANG, W., MUROFUSHI, N., KITAHARA, T., and KAMURO, Y. 1999. Opening of rice floret in rapid response to methyl jasmonate. J. Plant Growth Regul.18:153-158.

    PubMed  Google Scholar 

  • ZHU, J. K., HASEGAWA, P. M., and BRESSAN, R. A. 1997. Molecular aspects of osmotic stress in plants. Crit. Rev. Plant Sci.16:253-277.

    Google Scholar 

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Wang, Y., Mopper, S. & Hasenstein, K.H. Effects of Salinity on Endogenous Aba, Iaa, Ja, and Sa in Iris hexagona. J Chem Ecol 27, 327–342 (2001). https://doi.org/10.1023/A:1005632506230

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