Abstract
The production of manganese-dependent peroxidase (MnP) and lignin peroxidase (LiP) by the fungus Phanerochaete chrysosporium (ATCC 24725) in a new bioreactor, the Immersion Bioreactor, which grows cells under solid-state conditions, was studied. Maximum MnP and LiP activities were 987 U l−1 and 356 U l−1, respectively. The polymeric dye, Poly R-478, was degraded at 2.4 mg l−1 min−1 using the extracellular culture filtrate.
Similar content being viewed by others
References
Banat IM, Nigam P, Singh D, Marchant R (1996) Microbial decolorization of textile-dye-containing effluents: a review. Biores. Technol. 58: 217–227.
Bosco F, Ruggeri B, Sassi G (1996) Experimental identification of a scalable reactor configuration for lignin peroxidase production by Phanerochaete chrysosporium. J. Biotechnol. 52: 21–29.
Buckley KF, Dobson ADW (1998) Extracellular ligninolytic enzyme production and polymeric dye decolourization in immobilized cultures of Chrysosporium lignorum. Biotechnol. Lett. 20: 301–306.
Cannel E, Moo-Young M (1980) Solid-state fermentation systems. I. Process Biochem. June/July: 2–7.
Dosoretz CG, Chen HC, Grethlein HE (1990) Effect of environmental conditions on extracellular protease activity in ligninolytic cultures of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 56: 395–400.
Durand A, Renaud R, Almanza S, Maratray J, Diez M, Desgranges C (1993) Solid-state fermentation reactors: from lab scale to pilot plant. Biotechnol. Adv. 11: 591–597.
Ghose TK (1987) Measurement of cellulase activities. Pure Appl. Chem. 59: 257–268.
Gold MH, Kuwahara M, Chiu AA, Glenn JK (1984) Purification and characterization of an extracellular H2O2-requiring diarylpropane oxygenase from the white rot basidiomycete Phanerochaete chrysosporium. Arch. Biochem. Biophys. 234: 353–362.
Hesseltine CW (1977) Solid state fermentation. Part I. Process Biochem. 12: 24–27.
Jäger A, Croan C, Kirk TK (1985) Production of ligninases and degradation of lignin in agitated submerged cultures of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 50: 1274–1278.
Kim HY, Leem YE, Choi HT, Song HG (1995) Decolorization of dyes by white-rot fungi. Korean J. Mycol. 23: 298–304.
Kuwahara M, Glenn JK, Morgan MA, Gold MH (1984) Separation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Lett. 169: 247–250.
Laugero C, Sigoillot JC, Moukha S, Frasse P, Bellon-Fontaine MN, Bonnarme P, Mougin C, Asther M (1996) Selective hyperproduction of manganese peroxidases by Phanerochaete chrysosporium I-1512 immobilized on nylon net in a bubble-column reactor. Appl. Microbiol. Biotechnol. 44: 717–723.
Linko S (1991) Production of lignin peroxidase by immobilized Phanerochaete chrysosporium. MSc Thesis. Espoo, Finland: Helsinki University of Technology.
Lonsane BK, Ghildyal NP, Budiatman S, Ramakrishna SV (1985) Engineering aspects of solid state fermentation. Enzyme Microbiol. Technol. 7: 258–265.
Marsh AJ, Mitchell DA, Stuart DM, Howes T (1998) O2 uptake during solid-state fermentation in a rotating drum bioreactor. Biotechnol. Lett. 20: 607–611.
Marsh AJ, Stuart DM, Mitchell DA, Howes T (2000) Characterizing mixing in a rotating drum bioreactor for solid-state fermentation. Biotechnol. Lett. 22: 473–477.
Moreira MT, Feijoo G, Lema JM (1995) Production of manganese peroxidase by free pellets of Phanerochaete chrysosporium in an expanded-bed bioreactor. Biotechnol. Tech. 9: 371–376.
Moreira MT, Feijoo G, Palma C, Lema JM (1997) Continuous production of manganese peroxidase by Phanerochaete chrysosporium immobilized on polyurethane foam in a pulsed packed-bed bioreactor. Biotechnol. Bioeng. 56: 130–137.
Murthy RMV, Karanth NG, Raghava Rao KSMS (1993) Biochemical engineering aspects of solid-state fermentation. Adv. Appl. Microbiol. 38: 99–147
Niku-Paavola ML, Raaska L, Itävaara M (1990) Detection of whiterot fungi by a non-toxic stain. Mycol. Res. 94: 27–31.
Pandey A (1992) Recent process developments in solid-state fermentation. Process Biochem. 27: 109–117.
Rodríguez S, Santoro R, Cameselle C, Sanromán A (1997) Laccase production in semi solid state cultures of Phanerochaete chrysosporium. Biotechnol. Lett. 10: 995–998.
Rodríguez Couto S, Longo MA, Cameselle C, Sanromán A (1999) Ligninolytic enzymes from corncob cultures of Phanerochaete chrysosporium in semi solid state conditions. Acta Biotechnologica 19: 17–25.
Rodríguez Couto S, Santoro R, Cameselle C, Sanromán A (1998a) Effect of the different parts of the corncob employed as a carrier on ligninolytic activity in solid state cultures by Phanerochaete chrysosporium. Bioproc. Eng. 18: 251–255.
Rodríguez Couto S, Rättö M (1998b) Effect of veratryl alcohol and manganese (IV) oxide on ligninolytic activity in semi solid cultures of Phanerochaete chrysosporium. Biodegradation 9: 143–150
Rodríguez Couto S, Santoro R, Cameselle C, Sanromán A (2000) Ligninolytic enzyme production and the ability of decolourisation of Poly R-478 in packed-bed bioreactors by Phanerochaete chrysosporium. Bioproc. Eng. In press.
Tien M, Kirk TK (1983) Lignin-degrading enzyme from hymenomycete Phanerochaete chrysosporium Burds. Science 221: 661–663.
Tien M, Kirk TK (1984) Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization and catalytic properties of an unique H2O2-requiring oxygenase. Proc. Natl. Acad. Sci. USA 81: 2280–2284.
Tien M, Kirk TK (1988) Lignin peroxidase of Phanerochate chrysosporium. Meth. Enzymol. 16: 238–249.
Weatherburn MW (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 28: 971–974.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rivela, I., Rodríguez Couto, S. & Sanromán, A. Extracellular ligninolytic enzyme production by Phanerochaete chrysosporium in a new solid-state bioreactor. Biotechnology Letters 22, 1443–1447 (2000). https://doi.org/10.1023/A:1005607000999
Issue Date:
DOI: https://doi.org/10.1023/A:1005607000999