Abstract
We have cloned a 35-kDa protein from a mouse cDNA library with a 25% overall amino acididentity to yTom40 and 27% identity to nTom40. This homolog to Tom40 was named MOM35.It contains two possible start codons 36 amino acids apart from each other. Both the long andthe short version of MOM35 can be imported in vitro into mouse mitochondria. The identifiedprotein is imported into the outer mitochondrial membrane and comprises a trypsin-resistancepattern similar to that of nTom40. Tom40 of N. crassa, S. cerevisiae, and the protein identifiedherein contains a highly conserved region with possible physiological importance. Subsequentinvestigation has revealed that this region interacts specifically in vitro with preproteinsproposed to be imported by a Tom40-dependent pathway.
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Rivera, I.L., Shore, G.C. & Schleiff, E. Cloning and Characterization of a 35-kDa Mouse Mitochondrial Outer Membrane Protein MOM35 with High Homology to Tom40. J Bioenerg Biomembr 32, 111–121 (2000). https://doi.org/10.1023/A:1005524815130
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DOI: https://doi.org/10.1023/A:1005524815130