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Growth and lactic acid production in batch culture of Lactobacillus rhamnosus in a defined medium

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Abstract

To facilitate metabolic analysis, batch fermentations of Lactobacillus rhamnosus were carried out in a new defined medium. Biomass at 10.5 g/l and lactic acid at 67 g/l with a YP/S of 0.84 were achieved. The maximum specific growth rate and the average productivity were 0.49/h and 2.48 g/l.h, respectively. These are comparable to those of this organism and related organisms in complex media. Preliminary amino acid studies were also conducted, highlighting the importance of serine, asparagine, glutamine and cysteine. Kinetic analysis revealed that lactic acid production was predominantly growth-associated with growth associated and non-growth associated lactic acid constants of 0.389 mol/g-cell and 0.0025 mol/g-cell.h, respectively. Finally a kinetic model has been included to describe the fermentation of L. rhamnosus.

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Authors and Affiliations

  1. Biotechnology, The Flinders University of South Australia, Flinders Medical Centre, Bedford Park, 5042, South Australia, Australia

    Andrew R. Berry, Christopher M.M. Franco & Wei Zhang

  2. Department of Chemical Engineering, University of Cambridge, Pembroke Street, Cambridge, CB2 3RA, UK

    Anton P.J. Middelberg

Authors
  1. Andrew R. Berry
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  2. Christopher M.M. Franco
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  3. Wei Zhang
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  4. Anton P.J. Middelberg
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Berry, A.R., Franco, C.M., Zhang, W. et al. Growth and lactic acid production in batch culture of Lactobacillus rhamnosus in a defined medium. Biotechnology Letters 21, 163–167 (1999). https://doi.org/10.1023/A:1005483609065

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  • DOI: https://doi.org/10.1023/A:1005483609065

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