Skip to main content
Log in

Indigo formation by aromatic hydrocarbon-degrading bacteria

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A variety of aromatic hydrocarbon-degrading bacteria expressing different oxygenases were tested for their ability to produce indigo from indole. Styrene-grown cells of Pseudomonas putida S12 and CA-3 expressing styrene mono-oxygenase produced indigo at rates of 4–8 nmol min–1 mg dry wt–1. Toluene-grown cells of P. putida F1 and naphthalene-grown cells of P. putida PpG7 expressing dioxygenases formed indigo at rates of 1.5 and 2.5 nmol min–1 mg dry wt–1, respectively. © Rapid Science Ltd. 1998

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Allen, CCR, Boyd, DR, Larkin, MJ, Reid, KA, Sharma, ND and Wilson, K (1997). Appl Environ Microbiol 63:151–155

    Google Scholar 

  • Beltrametti, F, Marconi, AM, Bestetti, G, Colombo, C, Galli, E, Ruzzi, M and Zennaro, E, (1997). Appl Environ Microbiol 63:2232–2239

    Google Scholar 

  • Bialy, H (1997). Nature Biotechnology 15:110

    Google Scholar 

  • Berry, A, Battist, S, Chotani, G, Dodge, T, Peck, S, Power, S and Weyler, W (1995). In: Proceedings, Second Biomass Conference of the Americas: Energy, Enviroment, Agriculture, and Industry. pp1121–1129

  • Connors, N, Prevoznak, R, Chartrain, M, Reddy, J, Singhvi, R, Patel, Z, Olewinski, R, Salmon, P, Wilson, J and Greasham, R (1997) J Ind Microbiol 18:353–359

    Google Scholar 

  • Dunn, NW and Gunsalus, IC (1973). J Bacteriol 114:974–979

    Google Scholar 

  • Ensley, BD, Ratzkin, BJ, Osslund, TD, Simon, MJ, Wackett, LP and Gibson, DT (1983). Science 22:167–169

    Google Scholar 

  • Gibson, DT, Hensley, M, Yoshioka, H and Mabry, TJ (1970). Biochemistry 9:1626–1630

    Google Scholar 

  • Gray PMM (1928). Proc Royal Soc London, Ser B 102:2263–2279

    Google Scholar 

  • Hartmans, S, Smits, JP, van der Werf, MJ and de Bont JAM (1989). Appl Environ Microbiol 55:2850–2855

    Google Scholar 

  • Hartmans, S, van der Werf, MJ and de Bont JAM (1990). Appl Environ Microbiol 56:1347–1351

    Google Scholar 

  • Keil, H, Saint, CM and Williams, PA (1987). J Bacteriol. 169:764–770

    Google Scholar 

  • Kieboom, J, van de Brink, H, Frankena, J and de Bont, JAM (1996). In: Book of Abstracts of 6th Netherlands Biotechnology Congress, PH-12

  • Marconi, AM, Beltrametti, F, Bestetti, G, Solinas, F, Ruzzi, M, Galli, E and Zennaro, E (1996). Appl Environ Microbiol 62:121–127

    Google Scholar 

  • Mermod, N, Harayama, S and Timmis, KN (1986). Bio/Technology 4:321–324

    Google Scholar 

  • Murdock, D, Ensley, BD, Serdar, C and Thalen, M (1993). Bio/Technology 11:381–386

    Google Scholar 

  • Nozaki, M, Kagamiyama, H and Hayaichi, O (1963). Biochem Zeitschr 338:582–590

    Google Scholar 

  • O'Connor, K, Buckley, CM, Hartmans, S and Dobson, ADW (1995). Appl Environ Microbiol 61:544–548

  • O'Connor, KE, Dobson, ADW and Hartmans, S (1997). Appl Environ Microbiol 63:4287–4291

  • O'Leary, N, Marconi, AM and Dobson, ADW (1997). In: Abstracts book of VI International Congress on Pseudomonas: Molecular Biology and Biotechnolgy, Madrid, Spain, September 4–8, p.134

  • Wackett, LP and Gibson, DT (1988). Appl Environ Microbiol 54:1703–1708

    Google Scholar 

  • Warhurst, AM and Fewson, CA (1994). J Appl Bacteriol 77:597–606

    Google Scholar 

  • Williams, PJ and Murray, K (1974). J Bacteriol 120:416–423

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

O'Connor, K.E., Hartmans, S. Indigo formation by aromatic hydrocarbon-degrading bacteria. Biotechnology Letters 20, 219–223 (1998). https://doi.org/10.1023/A:1005361415496

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005361415496

Keywords

Navigation