Skip to main content
SpringerLink
Log in

A second gene for resistance to race 4 of Fusarium wilt in chickpea and linkage with a RAPD marker

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Fusarium wilt caused by Fusarium oxysporum Schlechtend.: Fr f. sp. ciceris (Padwick) Matuo & Sato is a devastating disease of chickpea. The current study was conducted to determine the inheritance of the gene(s) for resistance to race 4 of fusarium wilt and to identify linked RAPD markers using an early wilting line, JG-62, as a susceptible parent. Genetic analysis was performed on the F1s, F2s and F3 families from the cross of JG-62 × Surutato-77. The F3 families were inoculated with a spore suspension of the race 4 wilt pathogen and the results were used to infer the genotypes of the parent F2 plants. Results indicated that two independent genes controlled resistance to race 4. Linkage analysis of candidate RAPD marker, CS-27700, and the inferred F2 phenotypic data showed that this marker locus is linked to one of the resistance genes. Allelism indicated that the two resistance sources, Surutato-77 and WR-315, shared common alleles for resistance and the two susceptible genotypes, C-104 and JG-62, carried alleles for susceptibility. The PCR-based marker, CS-27700, was previously reported to be linked to the gene for resistance to race 1 in a different population which suggested that the genes for resistance to races 1 and 4 are in close proximity in the Cicer genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bhatti, M.A., 1990. The effects of inoculum density and environmental factors on wilt and root rot of chickpea (Cicer arietinum L.). Ph.D. Dissertation, Department of Plant Pathology, Washington State University, Pullman, Washington. UMI, A Bell & Howell Information Company, UMI number: 9102829. 132 p.

    Google Scholar 

  • Demeke, T., A. Laroche & D.A. Gaudet. A DNA marker for the Bt-10 common bunt resistance gene in wheat. Genome 39: 51-55.

  • Doyle, J.J. & J.L. Doyle, 1987. A rapid DNA isolation procedure for small amounts of fresh leaf tissue. Phytochem Bull 19: 11-15.

    Google Scholar 

  • El-Hadi, M., 1993. Studies on variability in morphology, pathogenicity and vegetative compatibility of Fusarium oxysporum f. sp. ciceris, and effects of inoculum density on chickpea wilt severity. M.Sc. Thesis. Department of Plant Pathology. Washington State University, Pullman, Washington. 118 p.

    Google Scholar 

  • Fritsch, P., M.A. Hanson, C.D. Spore, P.E. Pack & L.H. Rieseberg, 1993. Constancy of RAPD primer amplification strength among the distantly related taxa of flowering plants. Plant Mol Biol Rep 11: 10-20.

    CAS  Google Scholar 

  • Haware, M.P. & Y.L. Nene, 1982. Races of Fusarium oxysporum f. sp. ciceris. Plant Dis 66: 809-810.

    Article  Google Scholar 

  • Jiménez-Diaz, R.M., A Trapero-Casas & J. Cabrera de la Colina, 1989. Races of Fusarium oxysporum f. sp. ciceris infecting chickpea in southern Spain. In: E.C. Tjamos & C.H. Beckman (Eds), Vascular Wilt Diseases of Plants, pp. 515-520. NATO ASI SER. Vol. H28. Springer-Verlag, Berlin, Germany.

    Google Scholar 

  • Jiménez-Diaz, R.M., E. Perez-Artez & A.M. Nogales, 1992. Vegetative compatibility and self incompatibility within F. oxysporum f. sp. ciceris. 2nd International Food Legume Research Conference, 12-16 April, Cairo, Egypt. Poster Abstracts. p. 75.

  • Kesseli, R., H. Wiitsenboer, M. Stanghellini, G. Vandermark & R.W. Michelmore, 1993. Recessive resistance to Plasmopora lactucae-radicis maps by bulked segregant analysis to a cluster of dominant disease resistance genes in lettuce. Mol Plant-Microbe Interact 6: 722-728.

    CAS  Google Scholar 

  • Kumar, J. & M.P. Haware, 1982. Inheritance of resistance to fusarium wilt in chickpea. Phytopathology 72: 1035-1036.

    Article  Google Scholar 

  • Kumar, J. & M.P. Haware, 1983. Double podded chickpea genotype with resistance to fusarium wilt. International Chickpea Newsletter 8: 10-11.

    Google Scholar 

  • Lander, E.S., P. Green, J. Abrhamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newberg, 1987. Mapmaker: An interactive computer package for constructing primary linkage maps of experimental and natural populations. Genomics 1: 174-181.

    Article  PubMed  CAS  Google Scholar 

  • Mayer, M.S., A. Tullu, C.J. Simon, J. Kumar, W.J. Kaiser, J.M. Kraft & F.J. Muehlbauer, 1997. Development of a DNA marker for fusarium wilt resistance in chickpea. Crop Sci 37: 1625-1629.

    Article  CAS  Google Scholar 

  • Morales, G.J.A., 1986. Chickpea breeding program in Sonora, Mexico. International Chickpea Newsletter 15: 11-12.

    Google Scholar 

  • Muehlbauer, F.J. & K.B. Singh, 1987. Genetics of chickpea. pp. 99-125. In: M.C. Saxena & K.B. Singh (Eds), The Chickpea, C.A.B. International, Wallingford, Oxon, OX108DE, UK.

    Google Scholar 

  • Paran, I. & R.W. Michemore, 1993. Development of reliable PCR based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85: 985-993.

    Article  CAS  Google Scholar 

  • Sheldrake, A.R., N.P. Saxena & L. Krishnamurthy, 1978. The expression and contribution of double podded character in chickpea. Field Crops Research 1: 243-253.

    Article  Google Scholar 

  • Simon, C.J. & F.J. Muehlbauer, 1997. Construction of a chickpea linkage map and its comparison with maps of pea and lentil. J Hered 88: 115-119.

    CAS  Google Scholar 

  • Singh, H., J. Kumar, M.P. Haware & J.B. Smithson, 1987. Genetics of resistance to fusarium wilt in chickpeas. pp. 339-341. In: P.R. Day & G.J. Jellis (Eds), Genetics and Plant Pathogenesis. Blackwell Scientific Publications, Oxford. (Poster Abstracts).

    Google Scholar 

  • Snedecor, G.W. & W.G. Cochran, 1967. Statistical methods. Sixth edition. The Iowa State University Press, Ames, Iowa, USA. pp. 593.

    Google Scholar 

  • Tullu, A., 1996. Genetics of fusarium wilt resistance in chickpea. Ph.D. Dissertation. Crop and Soil Sciences Department, Washington State University, Pullman, WA, USA. UMI, A Bell & Howell Information Company, UMI number: 9806745. 152 pp.

    Google Scholar 

  • Tullu, A., F.J. Muehlbauer, W.J. Kaiser, C.J. Simon, M.S. Mayer, J. Kumar & J.M. Kraft, 1998. Inheritance and linkage of a gene for resistance to race 4 of Fusarium wilt and RAPD markers in chickpea. Euphytica 102: 227-232.

    Article  CAS  Google Scholar 

  • Upadhyaya, H.D., J.B. Smithson, M.P. Haware & J. Kumar, 1983a. Resistance to wilt in chickpea. I. Inheritance of late wilting in response to race 1. Euphytica 32: 447-452.

    Article  Google Scholar 

  • Upadhyaya, H.D., J.B. Smithson, M.P. Haware & J. Kumar, 1983b. Resistance to wilt in chickpea. II. Further evidence for two genes for resistance to race 1. Euphytica 32: 749-755.

    Article  Google Scholar 

  • Vidhyasekaran, P. & M. Muthamilan, 1995. Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis 79: 782-786.

    Article  Google Scholar 

  • Wechter, W.P., M.P. Whitehead, C.E. Thomas & R.A. Dean, 1995. Identification of a randomly amplified polymorphic DNA marker linked to the Fom 2 fusarium wilt resistance gene in muskmelon MR-1. Mol Plant Pathol 85: 1245-1249.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Crop and Soil Sciences, Washington State University, Pullman, Washington, 99164–6434, U.S.A

    A. Tullu, W.J. Kaiser & F.J. Muehlbauer

  2. U.S. Department of Agriculture, Agricultural Research Service, Prosser, Washington, 99350–9687, U.S.A

    J.M. Kraft

Authors
  1. A. Tullu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W.J. Kaiser
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J.M. Kraft
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F.J. Muehlbauer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tullu, A., Kaiser, W., Kraft, J. et al. A second gene for resistance to race 4 of Fusarium wilt in chickpea and linkage with a RAPD marker. Euphytica 109, 43–50 (1999). https://doi.org/10.1023/A:1003604209476

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1003604209476

Search

Navigation