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Changes in PAL, CHS, DAHP synthase (DS-Co and Ds-Mn) activity during anthocyanin synthesis in suspension culture of Fragaria ananassa

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Abstract

The activity of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase (DS-Mn, DS-Co), phenylalanine ammonia-lyase (PAL), and chalcone synthase (CHS) was monitored at various light intensities (dark, 8.88 μmol m−2 s−1, 88.8 μmol m−2 s−1) using a strawberry cell suspension culture. DS-Mn, PAL, and CHS were found to increase significantly (p>0.05) under light intensitie of 88.8 μmol m−2 s−1 compared to those of 8.88 μmol m−2 s−1 and dark. The activity of DS-Mn, PAL, and CHS were maximum at 88.8 μmol m−2 s−1. Anthocyanin content reached a maximum after 48–60 h of culturing at 88.8 μmol m−2 s−1. DS-Co showed greater activity than DS-Mn during cell culturing, but showed no correlation with anthocyanin production and light intensity. The CHS gene expression was continuous at a light intensity of 88.8 μmol m−2 s−1.

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  1. Japan; Fax: (81) 0138-43-5015; E-mail: tmori@pop.fish.hokudai.ac.jp)

    Tsukasa Mori, Miei Sakurai & Masaaki Sakuta

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  1. Tsukasa Mori
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  2. Miei Sakurai
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  3. Masaaki Sakuta
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Mori, T., Sakurai, M. & Sakuta, M. Changes in PAL, CHS, DAHP synthase (DS-Co and Ds-Mn) activity during anthocyanin synthesis in suspension culture of Fragaria ananassa . Plant Cell, Tissue and Organ Culture 62, 135–139 (2000). https://doi.org/10.1023/A:1026560223795

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