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Intraspecific Variation in Phytophthora citrophthora from Citrus Trees in Eastern Corsica

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Abstract

Isolates of Phytophthora pathogenic to citrus crops on Eastern Corsica and associated with gummosis were identified by PCR-RFLP of internal transcribed spacers (ITS) sequences and characterized by the random amplified microsatellites (RAMS) technique. A sample of 114 isolates collected from diseased trunks and fruits, and from soil, were overwhelmingly Phytophthora citrophthora. Further analysis indicated that the P. citrophthora population was not homogeneous in citrus groves. There were two groups, with a few (4%) atypical isolates in two marginal groups. The major groups have been re-examined in the light of mating behaviour, RFLPs of mitochondrial DNA and sequence comparisons of ITS regions of rDNA. They were found distinct with all these criteria and perhaps constitute distinct taxa. The results indicate that important modifications occurred in the population structure of P. citrophthora over time in Corsican groves. These changes may have impact on the recent outbreaks of gummosis.

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Author notes

  1. Séverine Cohen

    Present address: Equipe Evolution et Génétique des Populations Marines-CNRS UMR 7127; Station Biologique de Roscoff, BP 74, Place Georges Teissier, F-29682, Roscoff Cedex, France

  2. Christian Vernière

    Present address: CIRAD-FHLOR, TA50/PS4, F-34398, Montpellier Cedex 5, France

Authors and Affiliations

  1. Unité Interaction Plantes-Microorganismes et Santé Végétale, INRA, BP 2078, F-06606, Antibes Cedex, France

    Séverine Cohen, Valérie Allasia, Paul Venard, Sylvia Notter & Franck Panabières

  2. Station de Recherches Agronomiques de San Giuliano, INRA-CIRAD, F-20200, San Nicolao, Corse-France

    Christian Vernière

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  1. Séverine Cohen
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  2. Valérie Allasia
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  3. Paul Venard
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  4. Sylvia Notter
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  5. Christian Vernière
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  6. Franck Panabières
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Correspondence to Franck Panabières.

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Cohen, S., Allasia, V., Venard, P. et al. Intraspecific Variation in Phytophthora citrophthora from Citrus Trees in Eastern Corsica. European Journal of Plant Pathology 109, 791–805 (2003). https://doi.org/10.1023/A:1026190318631

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