Skip to main content
Log in

β-N-Oxalyl-L-α,β-Diaminopropionic Acid Protects the Activity of Glycolate Oxidase in Lathyrus sativus Seedlings under High Light

  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

The content of β-N-oxalyl-L-α,β-diaminopropionic acid (ODAP) and the activity of glycolate oxidase (GO) were positively correlated in the leaves of grass pea (Lathyrus sativus L.) seedlings. The activity of GO was kept at a steady level under the high light after treatment with ODAP. Although Na2S can activate GO, it cannot maintain the activity of GO under the high light. The content of ODAP increased and the activity of GO decreased with increasing oxalate concentration used for seedling treatment. The GO activity was high enough to keep photosynthesis at a steady level under high light. These findings suggested that Lathyrus sativus, using oxalate as a precursor to produce ODAP, protected the GO activity at high irradiance by scavenging the hydroxyl radicals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Campbell, C.G., Mehra, R.B., Agrawal, S.K., Chen, Y.Z., Abd El-Moneim, A.M., Khawaja, H.I.T., Yadov, C.R., Tay, J.V., and Araya, W.A., Current Status and Future Research Strategy in Breeding Grasspea (Lathyrus sativus), Euphytica, 1994, vol. 73, pp. 167–175.

    Google Scholar 

  2. Abd El-Moneim, A.E. and Cocks, P.S., Adaptation and Yield Stability of Selected Lines of Lathyrus spp. under Rainfed Conditions in West Asia, Euphytica, 1993, vol. 66, pp. 89–97.

    Google Scholar 

  3. Rao, S.L., Malathi, K.L., and Sarma, P.S., Lathyrism. World Review of Nutrition and Dietetics, 1969, vol. 10, pp. 214–238.

    Google Scholar 

  4. Ross, S.M., Roy, D.N., and Spencer, P.S., ?-N-Oxalylamino-1-Alanine Action on Glutamate Receptors, J. Neurochem., 1989, vol. 53, pp. 710–715.

    Google Scholar 

  5. Barber, J. and Andersson, B., Too Much of a Good Thing: Light Can Be Bad for Photosynthesis, Trends Biochem. Sci., 1992, vol. 17, pp. 61–66.

    Google Scholar 

  6. Malan, C., Greyling, M.M., and Gressel, J., Correlation between Cu, Zn-Superoxide Dismutase and Glutathione Reductase and Xenobiotic Stress Tolerance in Maize Inbreds, Plant Sci., 1990, vol. 69, pp. 157–166.

    Google Scholar 

  7. Dhindsa, R.S. and Matowe, W., Drought Tolerance in Two Mosses: Correlated with Enzymatic Defense against Lipid Peroxidation, J. Exp. Bot., 1981, vol. 32, pp. 79–91.

    Google Scholar 

  8. Burke, J.J., Gamble, P.E., Hatfield, J.L., and Quisenbery, J.E., Plant Morphological and Biochemical Responses to Field Water Deficits: 1. Responses of Glutathione Reductase Activity and Paraquat Sensitivity, Plant Physiol., 1985, vol. 79, pp. 415–419.

    Google Scholar 

  9. Xing Gengsheng, Cui Kairong, Li Ji, Wang Yafu, and Li Zhiziao, Water Stress and Accumulation of ?-N-Oxalyl-L-2-?,?-Diaminopropionic Acid in Grass Pea (Lathyrus sativus), J. Agric. Food Chem., 2001, vol. 49, pp. 216–220.

    Google Scholar 

  10. Zhou GongKe, Kong Yingzhen, Cui Kairong, Li Zhixiao, and Wang Yafu., Hydroxyl Radical Scavenging Activity of ?-N-Oxalyl-L-2-?,?-Diaminopropionic Acid, Phytochemisry, 2001, vol. 58, pp. 759–762.

    Google Scholar 

  11. Pfendel, E. and Bilger, W., Regulation and Possible Function of the Violaxanthin Cycle, Photosynth. Res., 1994, vol. 42, pp. 89–109.

    Google Scholar 

  12. Kozaki, A. and Takeba, G., Photorespiration Protects C3 Plants from Photooxidation, Nature, 1996, vol. 384, pp. 557–560.

    Google Scholar 

  13. Osmond, C., Badger, M., Maxwell, K., Bjorkman, K., and Leegood, R., Too Many Photorespiration and Photoinhibition and Photooxidation, Trends Plant Sci., 1997, vol. 2, pp. 119–121.

    Google Scholar 

  14. Katsushi, Y. and Mikio, N., Reduction to below Threshold Levels of Glycolate Oxidase Activities in Transgenic Tobacco Enhances Photoinhibition during Irradiation, Plant Cell Physiol., 2000, vol. 41, pp. 1397–1406.

    Google Scholar 

  15. Jerome, C.S. and William, L.O., Chemical Inhibition of the Glycolate Pathway in Soybean Leaf Cell, Plant Physiol., 1977, vol. 60, pp. 461–466.

    Google Scholar 

  16. Noll, C.R., Jr. and Burris, R.H., Nature and Distribution of Glycolic Acid Oxidase in Plant, Plant Physiol., 1954, vol. 29, pp. 261–265.

    Google Scholar 

  17. Feierabend, J., Developmental Studies on Microbodies in Wheat Leaves and the Photocontrol of Microbody Development, Planta, 1975, vol. 123, pp. 63–77.

    Google Scholar 

  18. Fei, W., Xiong, C., Qian, H., Xinchen, Q., and Zhixiao, L., Determination of Neurotoxin 3-N-Oxalyl-2,3-Diaminopropionic Acid and Nonprotein Amino Acids in Lathyrus sativus by Precolumn Derivatization with 1-Fluoro-2,4-Dinitrobenzene, J. Chromatogr., 2000, vol. 883, pp. 113–118.

    Google Scholar 

  19. Fumio, I., Akemi, Y., and Yu-Haey, K., Enzymation of 2.2-Diaminopropionic Acid, the Direct Precursor of the Neurotoxin ?-ODAP in Lathyrus sativus, Biol. Pharm, Bull., 1999, vol. 22, pp. 770–771.

    Google Scholar 

  20. Praveen, S., Joharl, R.G., and Bohnert, H.J., Cloning and Expression of OX-DARPO Degrading Genes from Soil Microbe, J. Plant Biochem. Biotechnol., 1997, vol. 3, pp. 25–29.

    Google Scholar 

  21. Hurkman, W. and Tanaka, C., Effect of Salt on Germin Gene Expression in Barley Roots, Plant Physiol., 1997, vol. 110, pp. 971–977.

    Google Scholar 

  22. Yamaguchi, K. and Mikio, N., Reduction to below Threshold Levels of Glycolate Oxidase Activities in Transgenic Tobacco Enhances Photoinhibition during Irradiation, Plant Cell Physiol., 2000, vol. 41, pp. 1397–1406.

    Google Scholar 

  23. Hideg, E., Spetea C., and Vass, I., Singlet Oxygen Production in Thylakoid Membranes during Photoinhibition as Detected by WPR Spectroscopy, Photosynth. Res., 1994, vol. 39, pp. 191–199.

    Google Scholar 

  24. Mehler, A.H., Studies on the Reaction of Illuminated Chloroplasts: 1. Mechanism of the Reduction of Oxygen and Other Hill Reagents, Arch. Biochem. Biophys., 1951, vol. 33, pp. 65–77.

    Google Scholar 

  25. Schaefer, L. and Jucrgen, F., Photoinactivation and Protection of Glycolate Oxides In Vitro and in Leaves, Z. Naturforsch., Sekt. C, 2000, vol. 55, pp. 361–372.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, J., Xing, G.M., Yan, Z.Y. et al. β-N-Oxalyl-L-α,β-Diaminopropionic Acid Protects the Activity of Glycolate Oxidase in Lathyrus sativus Seedlings under High Light. Russian Journal of Plant Physiology 50, 618–622 (2003). https://doi.org/10.1023/A:1025631906115

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1025631906115

Navigation