Skip to main content
SpringerLink
Log in

An oligosaccharide fraction from Korean mugwort herb suppresses death of the mouse thymocytes in culture by down-regulating the Fas death receptor gene

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Korean mugwort herb is a preparation of dried leaves from Artemisia species and has been used as a traditional medicine in Asia. An oligosaccharide fraction, AVF3, purified from the preparation promoted survival of the mouse thymocytes in culture. A mouse gene array study suggests that the AVF3 may modulate Fas/FasL dependent apoptotic cell death and thus has influence on the survival of the thymocytes in culture. RT-PCR analysis confirmed the down-regulation of the Fas gene by the AVF3 treatment, supporting that the AVF3 modulated thymocyte death by suppressing the Fas gene expression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barve SS, Kelkar SV, Gobejishvilli L, Joshi-Barve S, McClain CJ (2002) Mechanisms of alcohol-mediated CD4+ T lymphocyte death: relevance to HIV and HCV pathogenesis. Front. Biosci. 7: 1689–1696.

    Google Scholar 

  • Dai Z, Arakelov A, Wagener M, Konieczny BT, Lakkis FG (1999) The role of the common cytokine receptor gamma-chain in regulating IL-2-dependent, activation-induced CD8+ T cell death. J. Immunol. 163: 3131–3137.

    Google Scholar 

  • Hahm KB, Kim JH, You BM, Kim YS, Cho SW, Yim H, Ahn BO, Kim WB (1998) Induction of apoptosis with an extract of Artemisia asiatica attenuates the severity of cerulein-induced pancreatitis in rats. Pancreas 17: 153–157.

    Google Scholar 

  • Hwang JS, Chung HK, Bae EK, Lee AY, Ji HJ, Park DW, Jung HJ, Cho CW, Choi HJ, Lee DS, Lee KR, Youn HJ (2003) The polysaccharide fraction AIP1 from Artemisia iwayomogi suppresses apoptotic death of the mouse spleen cells in culture. Arch. Pharm. Res. 26: 294–300.

    Google Scholar 

  • Kawamura T, Azuma M, Kayagaki N, Shimada S, Yagita H, Okumura K (2000) Fas/Fas ligand-mediated apoptosis of murine Langerhans cells. J. Dermatol. Sci. 22: 96–101.

    Google Scholar 

  • Kim KH, Seong BL (2003) Pro-apoptotic function of HBV X protein is mediated by interaction with c-FLIP and enhancement of death-inducing signal. EMBO J. 22: 2104–2116.

    Google Scholar 

  • Kroemer G, Bosca L, Zamzami N, Merchetti P, Hortelano S, Martinez AC (1997) Detection of apoptosis and apoptosis-associated alterations. In: Lefkovits I. Immunology Methods Manual. San Diego: Academic Press, pp. 1111–1125.

    Google Scholar 

  • Middlebrook AJ, Martina C, Chang Y, Lukas RJ, DeLuca D (2002) Effects of nicotine exposure on T cell development in fetal thymus organ culture: arrest of T cell maturation. J. Immunol. 169: 2915–2124.

    Google Scholar 

  • Miura Y, Misawa N, Kawano Y, Okada H, Inagaki Y, Yamamoto N, Ito M, Yagita H, Okumura K, Mizusawa H, Koyanagi Y (2003) Tumor necrosis factor-related apoptosis-inducing ligand induces neuronal death in a murine model of HIV central nervous system infection. Proc. Natl. Acad. Sci. USA. 100: 2777–2782.

    Google Scholar 

  • Novelli F, Di Pierro F, Francia di Celle P, Bertini S, Affaticati P, Garotta G, Forni G (1994) Environmental signals influencing expression of the IFN-gamma receptor on human T cells control whether IFN-gamma promotes proliferation or apoptosis. J. Immunol. 152: 496–504.

    Google Scholar 

  • Taylor K (1995) A modification of the phenol sulfuric acid method of total sugar determination. Appl. Biochem. Biotechnol. 53: 207–214.

    Google Scholar 

  • Zeytun A, McKallip RJ, Fisher M, Camacho I, Nagarkatti M, Nagarkatti PS (2002) Analysis of 2,3,7,8-tetrachlorodibenzo-pdioxin-induced gene expression profile in vivo using pathwayspecific cDNA arrays. Toxicology 178: 241–260.

    Google Scholar 

  • Zhao M, Cribbs DH, Anderson AJ, Cummings BJ, Su JH, Wasserman AJ, Cotman CW (2003) The induction of the TNFalpha death domain signaling pathway in Alzheimer's disease brain. Neurochem. Res. 28: 307–318.

    Google Scholar 

  • Zhou H, Perkins SL, Tripp S, Hussong JW, Coffin CM (2002) Expression of apoptosis-related antigens, Fas, bcl-2, and p53, and Mib-1 proliferation index in the hypoplastic thymus of DiGeorge syndrome. Pediatr. Dev. Pathol. 5: 465–471.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Microbiology and School of Biotechnology & Biomedical Science, Korea

    Hye Kyung Chung, Eun Kyong Bae, Hee Jung Ji, Jung Suk Hwang, Dong Woon Park, Jung Eun Kim, Hwa Jin Jung & Hyun Joo Youn

  2. Department of Medical Technology, Inje University, Gimhae, 621-749, Korea

    Hye Kyung Chung, Eun Kyong Bae, Hee Jung Ji, Jung Suk Hwang, Dong Woon Park, Jung Eun Kim, Hwa Jin Jung, Hyun Ju Choi, Dong Seok Lee & Hyun Joo Youn

Authors
  1. Hye Kyung Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eun Kyong Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hee Jung Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jung Suk Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dong Woon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jung Eun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hwa Jin Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hyun Ju Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Dong Seok Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hyun Joo Youn
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyun Joo Youn.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kyung Chung, H., Kyong Bae, E., Jung Ji, H. et al. An oligosaccharide fraction from Korean mugwort herb suppresses death of the mouse thymocytes in culture by down-regulating the Fas death receptor gene. Biotechnology Letters 25, 1549–1553 (2003). https://doi.org/10.1023/A:1025482516404

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1025482516404

Search

Navigation