Abstract
Animals are constantly threatened by pathogenic microorganisms and have developed cellular and humoral immune responses to combat these infections. Invertebrates possess only an innate non-specific immune response. Antimicrobial substances are major components of innate immunity not only in invertebrates but also in vertebrates. Despite the importance of ticks as vectors of disease very little is known about their immune system. Our recent studies have revealed that four defensin isoforms are present in Ornithodoros moubata. These four isoforms are constitutively expressed in the midgut and up-regulated in response to blood feeding. Moreover, a mature peptide of defensin isoform A has been isolated from the tick midgut lumen. These findings indicate Ornithodoros defensins are involved in tick midgut defense against potentially harmful invasive microbes.
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Fogaca, A.C., da Silva Jr., P.I., Miranda, M.T., Bianch, A.G., Miranda, A., Ribolla, P.E. and Daffre, S. 1999. Antimicrobial activity of a bovine hemoglobin fragment in the tick Boophilus microplus. J. Biol. Chem. 274: 25330-25334.
Inoue, N., Hanada, K., Tsuji, N., Igarashi, I., Nagasawa, H., Mikami, T. and Fujisaki, K. 2001. Characterization of phagocytic hemocytes in Ornithodoros moubata (Acari: Ixodidae). J. Med. Entomol. 38: 514-519.
Johns, R., Sonenshine, D.E. and Hynes, W.L. 2000. Response of the tick Dermacentor variabilis (Acari: Ixodidae) to hemocoelic inoculation of Borrelia burgdorferi (Spirochetales). J. Med. Entomol. 37: 265-270.
Johns, R., Ohnishi, J., Broadwater, A., Sonenshine, D.E., De Silva, A.M. and Hynes, W.L. 2001a. Contrasts in tick innate immune responses to Borrelia burgdorferi challenge: immunotolerance in Ixodes scapularis versus immunocompetence in Dermacentor variabilis (Acari: Ixodidae). J. Med. Entomol. 38: 99-107.
Johns, R., Sonenshine, D.E. and Hynes, W.L. 2001b. Identification of a defensin from the hemolymph of the American dog tick, Dermacentor variabilis. Insect Biochem. Mol. Biol. 31: 857-865.
Kokoza, V., Ahmed, A., Cho, W.L., Jasinskiene, N., James, A.A. and Raikhel, A. 2000. Engineering blood meal-activated systemic immunity in the yellow fever mosquito, Aedes aegypti. Proc. Natl. Acad. Sci. USA 97: 9144-9149.
Kopacek, P., Vogt, R., Jindrak, L., Weise, C. and Safarik, I. 1999. Purification and characterization of the lysozyme from the gut of the soft tick Ornithodoros moubata. Insect Biochem. Mol. Biol. 29: 989-997.
Lehane, M.J., Wu, D. and Lehane, S.M. 1997. Midgut-specific immune molecules are produced by the blood-sucking insect Stomoxys calcitrans. Proc. Natl. Acad. Sci. USA 94: 11502-11507.
Lowenberger, C.A., Smartt, C.T., Bulet, P., Ferdig, M.T., Severson, D.W., Hoffmann, J.A. and Christensen, B.M. 1999. Insect immunity: molecular cloning, expression, and characterization of cDNAs and genomic DNA encoding three isoforms of insect defensin in Aedes aegypti. Insect Mol. Biol. 8: 107-118.
Nakajima, Y., van der Goes van Naters Yasui, A., Taylor, D. and Yamakawa, M. 2001. Two isoforms of a member of the arthropod defensin family from the soft tick, Ornithodoros moubata (Acari: Argasidae). Insect Biochem. Mol. Biol. 31: 747-751.
Nakajima, Y., Ogihara, K., Taylor, D. and Yamakawa, M. 2002. Antibacterial hemoglobin fragments from the midgut of the soft tick, Ornithodoros moubata (Acari: Argasidae). J. Med. Entomol. (in press).
Nakajima, Y., van der Goes van Naters Yasui, A., Taylor, D. and Yamakawa, M. 2003. Antibacterial peptide defensin is involved in midgut immunity of the soft tick, Ornithodoros moubata (Acari: Argasidae). Insect Mol. Biol. (in press).
Önfelt Tingvall, T., Roos, E. and Engström, Y. 2001. The imd gene is required for local Cecropin expression in Drosophila barrier epithelia. EMBO Rep. 2: 239-243.
Pereira, L.S., Oliveira, P.L., Barja-Fidalgo, C. and Daffre, S. 2001. Production of reactive oxygen species by hemocytes from the cattle tick Boophilus microplus. Exp. Parasitol. 99: 66-72.
Shahabuddin, M., Fields, I., Bulet, P., Hoffmann, J.A. and Miller, L.H. 1998. Plasmodium gallinaceum: differential killing of some mosquito stages of the parasite by insect defensin. Exp. Parasitol. 89: 103-112.
van der Goes van Naters Yasui, A., Taylor, D., Shono, T. and Yamakawa, M. 2000. Purification and partial amino acid sequence of antibacterial peptides from the hemolymph of the soft tick, Ornithodoros moubata (Acari: Argasidae). In: Proceedings of the 3rd International Conference on Ticks and Tick-borne Pathogens: Into the 21st Century, M. Kazimírová, M. Labuda and P.A. Nuttall (eds), pp. 189-194. Institute of Zoology, Slovak Academy of Sciences, Bratislava, Slovakia.
Vizioli, J., Richman, A.M., Uttenweiler-Joseph, S., Blass, C. and Bulet, P. 2001. The defensin peptide of the malaria vector mosquito Anopheles gambiae: antimicrobial activities and expression in adult mosquitoes. Insect Biochem. Mol. Biol. 31: 241-248.
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Nakajima, Y., Taylor, D. & Yamakawa, M. Involvement of Antibacterial Peptide Defensin in Tick Midgut Defense. Exp Appl Acarol 28, 135–140 (2002). https://doi.org/10.1023/A:1025399610947
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DOI: https://doi.org/10.1023/A:1025399610947