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A novel enzymatic route for biodiesel production from renewable oils in a solvent-free medium

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Abstract

A new enzymatic route for biodiesel production from soybean oil was developed using methyl acetate as a novel acyl acceptor. Novozym 435 (immobilized Candida antarctica lipase) gave the highest methyl ester (ME) yield of 92%. The optimum conditions of the transesterification were 30% enzyme based on oil weight; a molar ratio of methyl acetate/oil of 12:1; temperature 40 °C and reaction time 10 h. Since no glycerol was produced in the process, this method is very convenient for recycling the catalyst and by-product triacetylglycerol showed no negative effect on the fuel property.

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Authors and Affiliations

  1. Department of Chemical Engineering, Tsinghua University, Beijing, 100084, P.R. China

    Yuanyuan Xu, Wei Du, Dehua Liu & Jing Zeng

Authors
  1. Yuanyuan Xu
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  2. Wei Du
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  3. Dehua Liu
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  4. Jing Zeng
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Correspondence to Dehua Liu.

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Xu, Y., Du, W., Liu, D. et al. A novel enzymatic route for biodiesel production from renewable oils in a solvent-free medium. Biotechnology Letters 25, 1239–1241 (2003). https://doi.org/10.1023/A:1025065209983

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  • DOI: https://doi.org/10.1023/A:1025065209983

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