Abstract
Secretion of alkaline phosphatase (PhoA) encoded by a gene constituent of plasmids has been studied in Escherichia coli strains with controlled synthesis of anionic phospholipids (phosphatidylglycerol and cardiolipin, strain HDL11) and zwitterionic phospholipid (phosphatidylethanolamine, strain AD93). Changing the phospholipid composition of the membrane of these strains leads to an increase in secretion of PhoA, which is usually localized in the periplasm, into the culture medium. This correlates with a higher secretion of exopolysaccharides and lower content of lipopolysaccharide in the outer membrane. The results show the possibility of coupling protein secretion into the medium with biogenesis of cell envelope components in which phospholipids are involved.
Similar content being viewed by others
REFERENCES
Costerton, I. W., Ingram, J. M., and Cheng, K. J. (1974) Bacteriol. Rev., 38, 87-110.
Pugsley, A. P. (1993) Microbiol. Rev., 57, 50-108.
Nesmeyanova, M. A., Karamyshev, A. L., Tsfasman, I. M., and Fedotikova, E. T. (1991) Mol. Biol. (Moscow), 25, 770-778.
Nesmeyanova, M. A., Tsfasman, I. M., Karamyshev, A. L., and Suzina, N. E. (1991) World J. Microbiol. Biotechnol., 7, 394-406.
Nesmeyanova, M. A., Suzina, N. E., Tsfasman, I. M., and Badyakina, A. O. (1991) Mol. Biol. (Moscow), 25, 947-987.
Badyakina, A. O., Suzina, N. E., Dmitriyev, V. V., Tarakhovsky, Yu. S., Tsfasman, I. M., and Nesmeyanova, M. A. (1995) Biochemistry (Moscow), 60, 505-516.
Nesmeyanova, M. A., Kalinin, A. E., Karamishev, A. L., Mikhaleva, N. I., and Krupyanko, V. I. (1996) Proc. Biochem., 32, 1-7.
Bogdanov, M. V., Tarakhovsky, Yu. S., Manuvakhova, M. Sh., Gongadze, G. M., and Nesmeyanova, M. A. (1989) Biol. Membr. (Moscow), 6, 301-308.
Shibuya, I. (1992) Progr. Lipid Res., 31, 245-299.
Dowhan, W. (1997) Annu. Rev. Biochem., 66, 199-232.
Nesmeyanova, M. A., and Bogdanov, M. V. (1989) FEBS Lett., 257, 203-207.
De Kruijf, B. (1994) FEBS Lett., 346, 78-82.
Van Voorst, F., and de Kruijff, B. (2000) Biochem. J., 347, 601-612.
Lill, R., Dowhan, W., and Wickner, W. (1990) Cell, 60, 271-280.
Kajava, A. V., Bogdanov, M. V., and Nesmeyanova, M. A. (1991) J. Biomol. Struct. Dynamics, 9, 143-157.
Nesmeyanova, M. A., Karamyshev, A. L., Karamysheva, Z. N., Kalinin, A. E., Ksenzenko, V. N., and Kajava, A. V. (1997) FEBS Lett., 403, 203-207.
Golovastov, V. V., Mikhaleva, N. I., Kadirova, L. Yu., and Nesmeyanova, M. A. (2000) Biochemistry (Moscow), 65, 1097-1104.
Mikhaleva, N. I., Golovastov, V. V., Zolov, S. N., Bogdanov, M. V., Dowhan, W., and Nesmeyanova, M. A. (2001) FEBS Lett., 493, 85-90.
Boyd, D., Guan, D. D., Willard, S., Wright, W., Strauch, K., and Beckwith, J. (1987) in Phosphate Metabolism and Cellular Regulation in Microorganisms (Torriani-Gorini, A., Rothman, F. G., Silver, S., Wright, A., and Yagil, E., eds.) American Society for Microbiology, Washington, DC, pp. 89-93.
De Chavigny, A., Heacock, P. N., and Dowhan, W. (1991) J. Biol. Chem., 66, 5323-5332.
Kusters, R., Dowhan, W., and de Kruijff, B. (1991) J. Biol. Chem., 266, 8659-8662.
Zolov, S. N., Mikhaleva, N. I., Kalinin, A. E., and Nesmeyanova, M. A. (2002) Biochemistry (Moscow), 67, 872-879.
Guzman, B. D., Carson, M. J., and Beckwith, J. (1995) J. Bacteriol., 177, 4121-4130.
Inouye, H., Michaelis, S., Wright, A., and Beckwith, J. (1981) J. Bacteriol., 146, 668-675.
Torriani-Gorini, A. (1966) in Procedures in Nucleic Acid Research (Cantoni, G. L., and Davis, R., eds.) Harper and Row, N. Y., pp. 224-234.
Loyda, Z., Gossrau, R., and Shibler, T. (1982) Histochemistry of Enzymes [Russian translation], Mir, Moscow.
Miura, T., and Mizushima, S. (1968) Biochim. Biophys. Acta, 150, 159-161.
Schnaitman (1971) J. Bacteriol., 108, 545-552.
Laemmli, U. K. (1970) Nature, 227, 680-685.
Davis, R. I. (1964) Annu. N. Y. Acad. Sci., 121, 404-427.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) J. Biol. Chem., 193, 265-275.
Zakharova, I. Ya., and Kosenko, L. V. (1982) Methods of Study of Microbial Polysaccharides [in Russian], Naukova Dumka, Kiev, pp. 37-38.
Westphal, O., and Luderitz, Z. (1959) J. Biol. Chem., 234, 705-709.
Golovastov, V. V., Zolov, S. N., and Nesmeyanova, M. A. (2002) Biochemistry (Moscow), 67, 978-985.
Rietveld, A. G., Killian. A., Dowhan, W., and Ben de Kruijff, B. (1993) J. Biol. Chem., 268, 12427-12433.
Rietveld, A. G., Chupin, V. V., Koorengevel, M. C., Wienk, H. L., Dowhan, W., and de Kruijff, B. (1994) J. Biol. Chem., 269, 28670-28675.
Mikhaleva, N. I., Zolov, S. N., Suzina, N. E., Melkozernov, A. N., and Nesmeyanova, M. A. (1995) Biochemistry (Moscow), 60, 881-888.
Nakata, A., Shinagawa, H., and Rothman, F. (1987) in Phosphate Metabolism and Cellular Regulation in Microorganisms (Torriani-Gorini, A., Rothman, F., Silver, S., Wright, A., and Yagil, E., eds.) American Society for Microbiology, Washington, DC, pp. 139-141.
Nesmeyanova, M. A., Maraeva, O. B., Severin, A. I., and Kulaev, I. S. (1978) Folia Microbiol., 23, 30-36.
Nesmeyanova, M. A., Motlokh, O. B., Kolot, M. N., and Kulaev, I. S. (1981) J. Bacteriol., 146, 453-459.
Tsfasman, I. M., Badyakina, A. O., Nagornaya, N. E., and Nesmeyanova, M. A. (1993) Mol. Biol. (Moscow), 27, 805-816.
Golovastov, V. V., Mikhaleva, N. I., and Nesmeyanova, M. A. (2002) Biochemistry (Moscow), 67, 765-769.
Nicaido, H., and Varra, M. (1985) Microbiol. Rev., 49, 1-32.
Dowhan, W. (1998) Biochim. Biophys. Acta, 1376, 455-466.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Badyakina, A.O., Koryakina, Y.A., Suzina, N.E. et al. Membrane Phospholipid Composition of Escherichia coli Affects Secretion of Periplasmic Alkaline Phosphatase into the Medium. Biochemistry (Moscow) 68, 752–759 (2003). https://doi.org/10.1023/A:1025030932657
Issue Date:
DOI: https://doi.org/10.1023/A:1025030932657