biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:535-541, 2003 | DOI: 10.1023/A:1024811527499

The Role of Abscisic Acid in Acclimation of Plants Cultivated in vitro to ex vitro Conditions

M. Hronková1,3, H. Zahradníčková2, M. Šimková1, P. Šimek2, A. Heydová2
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
2 Institute of Entomology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
3 Faculty of Biology, University of South Bohemia, České Budějovice, Czech Republic

The content of endogenous free abscisic acid (ABA) in the shoots of in vitro cultivated tobacco (Nicotiana tabacum L. cv. White Burley) and its changes during ex vitro acclimation of these plants to the greenhouse or growth chamber were estimated. The content of free ABA significantly increased at the 1st and/or 2nd day after plant transfer from in vitro to ex vitro. The ABA content of plants covered with transparent foil to maintain higher relative humidity (RH), did not significantly differ from ABA content of plants cultivated under ambient RH. Transfer to fresh medium also transiently increased the content of endogenous ABA. The ABA content in plants, which had been acclimated for 1 week to ex vitro conditions, decreased to the content found in the in vitro plants. Acclimation to ex vitro conditions affected the stomata on adaxial and abaxial sides differently: stomata on the adaxial side were less open than those on the abaxial one. The exogenous application of 5 μM ABA increased transiently its endogenous concentration in shoots of in vitro plants more than ten fold, but after 1 week the concentration in the shoots decreased.

Keywords: Nicotiana tabacum; stomata
Subjects: abscisic acid (ABA); acclimation from in vitro to ex vitro culture; in vitro culture, acclimation from in vitro to ex vitro culture; Nicotiana tabacum; stomata opening, acclimation from in vitro to ex vitro culture; tobacco, acclimation from in vitro to ex vitro culture

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation

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Hronková, M., Zahradníčková, H., Šimková, M., Šimek, P., & Heydová, A. (2003). The Role of Abscisic Acid in Acclimation of Plants Cultivated in vitro to ex vitro Conditions. Biologia plantarum, 46(4), 535-541. doi: 10.1023/A:1024811527499
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