Abstract
Leaf scald is a vascular disease of sugarcane caused by Xanthomonas albilineans. Scalded leaves show white-yellowish streaks alternating with green zones in parallel to the main veins. These zones develop large bulliform cells, probably as a consequence of the wilting process. Moreover, a gum exudate occludes phloem and bundle vessels, and partially enters mesophyll cells. Some lysigenic cavities appear near the xylem. However, the white-yellowish streaks show both phloem and xylem completely occluded by the gum and the overall mesophyll appears to be full of this bacterial secretion, as revealed by scanning electron microscopy. The gum in conducting tissues has been purified from juice obtained from scalded stalks by precipitation with isopropyl alcohol and size-exclusion chromatography. It was identified as a xanthan-like polysaccharide and found to be composed of glucose, mannose and glucuronic acid by acidic hydrolysis and capillary electrophoresis.
Similar content being viewed by others
References
Birch RG and Patil SS (1985) Preliminary characterization of an antibiotic produced by Xanthomonas albilineans which inhibits DNA synthesis in Escherichia coli. Journal of General Microbiology 131: 1069-1075
Bowes BG (1996) A Colour Atlas of Plant Structure, Manson Publishers, London
Chang KW, Weng SF and Tseng YH (2001) UDP-glucose dehydrogenase gene of Xanthomonas campestris is required for virulence. Biochemical and Biophysical Research Communications 287: 550-555
Christensen BE and Smidsrod O (1996) Dependence of the content of unsubstituted (cellulosic) regions in prehydrolyzed xanthans on the rate of hydrolysis by Trichoderma reesei endoglucanase. International Journal of Biological Macromolecules 18: 93-99
da Silva FR, Vettore AL, Kemper EL, Leite A and Arruda P (2001) Fastidian gum: The Xylella fastidiosa exopolysaccharide possibly involved in bacterial pathogenicity. FEMS Microbiological Letters 203: 165-171
de Armas R, Martínez M, Vicente C and Legaz ME (1999) Free and conjugated polyamines and phenols in raw and alkalineclarified sugarcane juices. Journal of Agricultural and Food Chemistry 47: 3086-3092
Dubois M, Guilles KA, Hamilton JK, Rebers PA and Smith D (1956) Colorimetric method for determination of sugar and related substances. Analytical Chemistry 28: 350-356
Esau H (1977) Anatomy of Seed Plants, 2nd edn, John Wiley & Sons, New York
Galindo E and Albiter V (1996) High-yield recovery of xanthan by precipitation with isopropyl alcohol in a stirred tank. Biotechnology Progress 12: 540-547
Gunning BES and Steer MW (1986) Plant Cell Biology. An Ultrastructural Approach, Edward Arnold, London
Huang GZ, Zhang LH and Birch RG (2000a) Albicidin antibiotic and phytotoxin biosynthesis in Xanthomonas albilineans requires a phosphopantetheinyl transferase gene. Gene 258: 193-199
Huang GZ, Zhang LH and Birch RG (2000b) Analysis of the genes flanking xabB: A methyltransferase gene is involved in albicidin biosynthesis in Xanthomonas albilineans. Gene 255: 327-333
Hugouvieux V, Barber CE and Daniels MJ (1998) Entry of Xanthomonas campestris pv campestris into hydathodes of Arabodipsis thaliana leaves:Asystem for studying early infection events in bacterial pathogenesis. Molecular Plant Microbe Interactions 11: 537-543
Katzen F, Ferreiro DU, Oddo CG, Ielmini MV, Becker A, Puhler A, Ielpi L (1998) Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence. Journal of Bacteriology 180: 1607-1617
Legaz ME and Pedrosa MM (1993) Separation of acidic proteins by capillary zone electrophoresis and size-exclusion HPLC: A comparison. Journal of Chromatography A 655: 21-29
Li YZ, Tang JL, Tang DJ and Ma QS (2001) Pathogenicity of EPS-deficient mutants (gumB(?), gumD(?) and gumE(?)) of Xanthomonas campestris pv. campestris. Progress in Natural Science 11: 871-875
Lopes SA, Damann KE and Grelen LB (1998) Comparison of methods for identification of the sugarcane pathogen Xanthomonas albilineans. Summa Phytopathologica 24: 114-119
Martin JP and Robinson PE (1961) Leaf scald. In: Hughes CG, Abbott EV and Wismer CA (eds) Sugarcane Diseases of the World, Vol 1 (pp 79-107) Elsevier, Amsterdam
Purseglove JW (1979) Tropical Crops: Monocotyledons, Longman Group Ltd., London
Rott P, Mohamed IS, Klett P, Soupa D, de Saint Albin A, Feldmann P and Letourmy P (1997) Resistance to leaf scald disease is associated with limited colonization of sugarcane and wild relatives by Xanthomonas albilineans. Phytopathology 87: 1202-1213
Sánchez A, Ramirez ME, Torres LG and Galindo E (1997) Characterization of xanthans from selected Xanthomonas strains cultivated under constant dissolved oxygen. World Journal of Microbiology and Biotechnology 13: 443-451
Wallis FM, Rijkenberg FHJ, Joubert JJ and Martin MM (1973) Ultrastructural histopathology of cabbage leaves infected with Xanthomonas campestris. Physiological Plant Pathology 3: 371-378
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Solas, M.T., Piñón, D., Acevedo, R. et al. Ultrastructural Changes and Production of a Xanthan-Like Polysaccharide Associated with Scald of Sugarcane Leaves Caused by Xanthomonas albilineans . European Journal of Plant Pathology 109, 351–359 (2003). https://doi.org/10.1023/A:1023533812304
Issue Date:
DOI: https://doi.org/10.1023/A:1023533812304