Abstract
Two yeasts, Debaryomyces polymorphus, Candida tropicalis, and two filamentous fungi, Umbelopsis isabellina, Penicillium geastrivorus, could completely decolorize 100 mg Reactive Black 5 (RB 5) l−1 within 16–48 h. Manganese-dependent peroxidase (MnP) activities between 60 and 424 U l−1 were detected in culture supernatants of three of these organisms indicating the color removal by enzymatic biodegradation but with P. geastrivorus there was no ligninolytic enzyme activity in its culture and the decolorization was mainly due to biosorption to mycelium. Extensive decolorization by D. polymorphus (69–94%) and C. tropicalis (30–97%) was obtained with five other azo dyes and one anthraquinone dye. Except for Reactive Brilliant Blue KNR and Reactive Yellow M-3R, the four azo dyes, Reactive Red M-3BE, Procion Scharlach H-E3G, Procion Marine H-EXL and Reactive Brilliant Red K-2BP, induced D. polymorphus to produce MnP (105–587 U l−1). However, MnP activities of 198–329 U l−1 were only detected in the culture of C. tropicalis containing Reactive Red M-3BE and Reactive Brilliant Red K-2BP, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
An SY, Min SK, Cha IH, Choi YL, Cho YS, Kim CH, Lee YC (2002) Decolorization of triphenylmethane and azo dyes by Citrobacter sp. Biotechnol. Lett. 24: 1037–1040.
Fu Y, Viraraghavan T (2001) Fungal decolorization of dye wastewaters: a review. Bioresour. Technol. 79: 251–262.
Glenn JK, Gold MH (1983) Decolorization of several polymeric dyes by the lignin-degrading basidiomycete Phanerochaete chrysosporium. Appl. Environ. Microbiol. 45: 1741–1747.
Heinfling A, Bergbauer M, Szewzyk U (1997) Biodegradation of azo and phthalocyanine dyes by Trametes versicolor and Bjerkandera adusta. Appl. Microbiol. Biotechnol. 48: 261–266.
Kurtzman CP, Robnett CJ (1997) Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5′ end of the large-subunit (26S) ribosomal DNA gene. J. Clin. Microbiol. 35: 1216–1223.
Lang E, Eller G, Zadrazil F (1997) Lignocellulose decomposition and production of ligninolytic enzymes during interaction of white rot fungi with soil microorganism. Microb. Ecol. 34: 1–10.
Martin H (2002) Review: lignin conversion by manganese peroxidase (MnP). Enzyme Microb. Technol. 30: 454–466.
Robinson T, McMullan G, Marchant R, Nigam P (2001) Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresour. Technol. 77: 247–255.
Shintani N, Sugano Y, Shoda M (2002) Decolorization of kraft pulp bleaching effluent by a newly isolated fungus Geotrichum candidum Dec 1. J. Wood Sci. 48: 402–408.
Tien M, Kirk TK (1988) Lignin peroxidases of Phanerochaete chrysosporium. Meth. Enzymol. 161: 238–249.
Voigt K, Cegelink E, O'Donnell K (1999) Phylogeny and PCR identification of clinically important zygomycetes based on nuclear ribosomal-DNA sequence data. J. Clin. Microbiol. 37: 3957–3964.
White TJ, Bruns TD, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal DNA for phylogenetics. In: PCR Protocols: A Guide to the Methods and Applications. New York: Academic Press, p. 315.
Zhang F, Knapp JS, Tapley KN (1999) Decolorization of cotton bleaching effluent with a wood rotting fungus. Water Res. 33: 919–928.
Zheng Z, Levin RE, Pinkham JL, Shetty K (1999) Decolorization of polymeric dyes by a novel Penicillium isolate. Proc. Biochem. 34: 31–37.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yang, Q., Yang, M., Pritsch, K. et al. Decolorization of synthetic dyes and production of manganese-dependent peroxidase by new fungal isolates. Biotechnology Letters 25, 709–713 (2003). https://doi.org/10.1023/A:1023454513952
Issue Date:
DOI: https://doi.org/10.1023/A:1023454513952