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The influence of a dietary lipid source on growth, muscle fatty acid composition and erythrocyte osmotic fragility of hybrid tilapia

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Abstract

An 8-week feeding trial was conducted to determine the effects of various dietary lipids on the growth, tissue proximate composition, muscle fatty acid composition and erythrocyte osmotic fragility of red hybrid tilapia, Oreochromis sp. Five isonitrogenous and isoenergetic semipurified diets were supplemented with 10% of either cod liver oil (CLO), sunflower oil (SFO), crude palm oil (CPO), crude palm kernel oil (CPKO), or a combination of 5% CLO with 5% palm fatty acid distillates (PFAD), respectively. There were no significant effects (P > 0.05) of diet on growth but fish fed the CLO diet showed a significantly (P< 0.05) poorer feed efficiency ratio compared to fish fed the CPO diet. Lipid deposition in fish muscle was mostly similar among fish fed the various diets but bone ash was significantly higher in fish fed the CPO and CPKO diets. Muscle lipids of fish fed palm oil-based diets did not increase in saturated fatty acids content but showed significantly lower polyunsaturated fatty acid (PUFA) concentrations compared to fish fed the CLO diet. The concentrations of individual PUFA in muscle lipids were strongly influenced by dietary PUFA concentrations. Dietary lipids did not markedly affect the structural integrity of erythrocyte membranes but the erythrocytes of tilapia fed the CPO diet were slightly more resistant to osmotic lysis. It was concluded that palm oil products, especially CPO, could be successfully used in the diet of hybrid tilapia based on its availability, cheaper costs and its potential ability to enhance oxidative stability due to its low PUFA content and high natural concentrations of antioxidants.

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REFERENCES

  • Al-Owafeir, M.A. and Belal, I.E.H. 1996. Replacing palm oil for soybean oil in tilapia, Oreochromis niloticus (L.), feed. Aquacult. Res. 27: 221–224.

    Google Scholar 

  • AOAC (Association of Official Analytical Chemists) 1997. Official Methods of Analysis of AOAC International. 16th edn. AOAC International, Arlington, Virginia.

    Google Scholar 

  • Barlow, S. 2000. Fishmeal and fish oil: Sustainable feed ingredients for aquafeeds. Global Aquacult. Advocate 4: 85–88.

    Google Scholar 

  • Bell, J.G., McVicar, A.H., Park, M.T. and Sargent, J.R. 1991. High dietary linoleic acid affects the fatty acid compositions of individual phopholipids from tissues of Atlantic salmon (Salmo salar): association with stress susceptibility and cardiac lesion. J. Nutr. 121: 1163–1172.

    Google Scholar 

  • Bell, J.G., McEvoy, J., Tocher, D.R. and Sargent, J.R. 2000. Depletion of á-tocopherol and astaxanthin in Atlantic salmon (Salmo salar) affects autooxidative defense and fatty acid metabolism. J. Nutr. 130: 1800–1808.

    Google Scholar 

  • Bell, J.G., Henderson, R.J., Tocher, D.R., McGhee, F., Dick, J.R., Porter, A., Smullen, R.P. and Sargent, J.R. 2002. Substituting fish oil with crude palm oil in the diet of Atlantic salmon (Salmo salar) affects muscle fatty acid composition and hepatic fatty acid metabolism. J. Nutr. 132: 222–230.

    Google Scholar 

  • Bligh, E.G. and Dyer, W.J. 1959. A rapid method for total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.

    Google Scholar 

  • Chou, B.S. and Shiau, S.Y. 1999. Both n-6 and n-3 fatty acids are required for maximal growth of juvenile hybrid tilapia. North American J. Aquacult. 61: 13–20.

    Google Scholar 

  • Chou, B.S., Shiau, S.Y. and Hung, S.S.O. 2001. Effect of dietary cod liver oil on growth and fatty acids of juvenile hybrid tilapia. North American J. Aquacult. 63: 277–284.

    Google Scholar 

  • Duncan, D. 1955. Multiple range tests and multiple F tests. Biometrics 11: 1–42.

    Google Scholar 

  • Ezell, G.H., Sulya, L.L. and Dodgen, C.L. 1969. The osmotic fragility of some fish erythrocytes in hypotonic saline. Comp. Biochem. Physiol. 28: 409–415.

    Google Scholar 

  • FAO (Food and Agriculture Organization of the United Nations) 1999. Aquaculture production statistics 1988–1997. FAO Fisheries Circular No. 815, Rev. 11. FAO Rome, 203 pp.

    Google Scholar 

  • Greene, D.H.S. and Selivonchick, D.P. 1990. Effects of dietary vegetable, animal and marine lipids on muscle lipid and hematology of rainbow trout (Oncorhynchus mykiss). Aquaculture 89: 165–182.

    Google Scholar 

  • Henderson, R.J. 1996. Fatty acid metabolism in freshwater fish with particular reference to polyunsaturated fatty acids. Arch. Anim. Nutr. 49: 5–22.

    Google Scholar 

  • Huang C.H., Huang, M.C. and Hou, P.C. 1998. Effect of dietary lipids on fatty acid composition and lipid peroxidation in sarcoplasmic reticulum of hybrid tilapia, Oreochromis niloticus × O. aureus. Comp. Biochem. Physio. 120: 331–336.

    Google Scholar 

  • Kanazawa, A., Teshima, S., Sakamoto, M. and Awal M.A. 1980. Requirements of Tilapia zillii for essential fatty acids. Bull. Jpn. Soc. Sci. Fish. 46: 1353–1356.

    Google Scholar 

  • Kiron, V., Takeuchi, T. and Watanabe, T. 1994. The osmotic fragility of erythrocytes in rainbow trout under different dietary fatty acid status. Fish. Sci. 60: 93–95.

    Google Scholar 

  • Klinger, R.C., Blazer, V.S. and Echevarria, C. 1996. Effects of dietary lipid on the hematology of channel catfish, Ictalurus punctatus. Aquaculture 147: 225–233.

    Google Scholar 

  • Legendre, M., Kerdchuan, N., Corraze, G. and Bergot, P. 1995. Larval rearing of an African catfish Heterobranchus longifilis (Teleostei, Clariidae): effect of dietary lipids on growth, survival and fatty acid composition of fry. Aquat. Living Resour. 8: 355–363.

    Google Scholar 

  • Lim, P.K., Boey, P.L. and Ng, W.K. 2001. Dietary palm oil level affects growth performance, protein retention and tissue vitamin E concentration of African catfish, Clarias gariepinus. Aquaculture 202: 101–112.

    Google Scholar 

  • NRC (National Research Council) 1993. Nutrient Requirements of Fish. National Academy Press, Washington, DC, 114 pp.

    Google Scholar 

  • Ng, W.K., Tee, M.C. and Boey, P.L. 2000. Evaluation of crude palm oil and refined palm olein as dietary lipids in pelleted feeds for a tropical bagrid catfish Mystus nemurus (Cuvier & Valenciennes). Aquacult. Res. 31: 337–347.

    Google Scholar 

  • Ng, W.K., Lim, P.K. and Boey, P.L. 2003. Dietary lipid and palm oil source affects growth, fatty acid composition and muscle á-tocopherol concentration of African catfish, Clarias gariepinus. Aquaculture 215: 229–243.

    Google Scholar 

  • Sambanthamurthi, R., Sundram, K. and Tan, Y.A. 2000. Chemistry and biochemistry of palm oil. Progress Lipid Res. 39: 507–558.

    Google Scholar 

  • Scaife, J.R., Onibi, G.E., Murray, I., Fletcher, T.C. and Houlihan, D.F. 2000. Influence of á-tocopherol acetate on short-and longterm storage properties of fillets from Atlantic salmon Salmo salar fed a high lipid diet. Aquacult. Nutr. 6: 65–71.

    Google Scholar 

  • Serbinova, E., Kagan, V., Han, E. and Packer, L. 1991. Free radical recycling and intramembrane mobility in the antioxidant properties of alpha-tocopherol and alpha-tocotrienol. Free Rad. Bio. Med. 10: 263–275.

    Google Scholar 

  • Siew, W.L., Chong, C.L. and Tan, Y.A. 1995. Composition of the oil in palm kernel from Elaeis guineensis. J. Am. Oil Chem. Soc. 72: 1587–1589.

    Google Scholar 

  • Steffens, W. 1997. Effects of variation in essential fatty acids in fish feeds on nutritive value of freshwater fish for humans. Aquaculture 151: 97–119.

    Google Scholar 

  • Stickney, R.R., McGreachin, R.B., Lewis, D.H. and Marks, J. 1983. Response of young channel catfish to diets containing purified fatty acids. Trans. Am. Fish. Soc. 112: 665–669.

    Google Scholar 

  • Takeuchi, T., Satoh, S. and Watanabe, T. 1983. Requirement of Tilapia nilotica for essential fatty acids. Bull. Jpn. Soc. Sci. Fish. 49: 1127–1134.

    Google Scholar 

  • Tortensen, B.E., Lie, O. and Froyland, L. 2000. Lipid metabolism and tissue composition in Atlantic salmon (Salmo salar L.) – effects of capelin oil, palm oil and oleic-enriched sunflower oil as dietary lipid sources. Lipids 35: 653–664.

    Google Scholar 

  • Watkins, B.A., Li, Y., Rogers, L.L., Hoffman, W.E, Iwakiri, Y. Allen, K.G.D. and Seifert, M.F. 2001. Effect of red palm olein on bone tissue fatty acid composition and histomorphometric parameters. Nutr. Res. 21: 199–213.

    Google Scholar 

  • Young, F.V.K. 1987. Refining and fractionation of palm oil. In: Palm Oil: Critical Reports on Applied Chemistry, Vol. 15, pp. 39–69. Edited by F.D. Gunstone. Wiley, New York.

    Google Scholar 

  • Yu, T.C., Sinnhuber, R.O. and Putnam, G.B. 1977. Effect of dietary lipids on fatty acid composition of body lipid in rainbow trout (Salmo gairdneri). Lipids 12: 495–499.

    Google Scholar 

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Authors and Affiliations

  1. Fish Nutrition Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang, 11800, Malaysia

    Wing-Keong Ng, Phaik-Kin Lim & Hadizah Sidek

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  1. Wing-Keong Ng
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  2. Phaik-Kin Lim
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Ng, WK., Lim, PK. & Sidek, H. The influence of a dietary lipid source on growth, muscle fatty acid composition and erythrocyte osmotic fragility of hybrid tilapia. Fish Physiology and Biochemistry 25, 301–310 (2001). https://doi.org/10.1023/A:1023271901111

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