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Low concentrations of NaHSO3 increase cyclic photophosphorylation and photosynthesis in cyanobacterium Synechocystis PCC6803

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Abstract

Application of NaHSO3 solution at low concentrations (20–200 μM) to the culture medium enhanced photosynthetic oxygen evolution in cyanobacterium Synechocystis PCC6803 by more than 10%. The slow phase of ms-DLE was strengthened, showing that the transmembrane proton motive force related to photophosphorylation was enhanced. It was also observed that dry weight as well as ATP content under illuminated conditions were both increased after the treatment, indicating that low concentrations of NaHSO3 could enhance the supply of ATP and thus increase biomass accumulation. In accord with the promotion in the photosynthetic oxygen evolution and ATP content, the transient increase in chlorophyll fluorescence after the termination of actinic light was increased; and meanwhile, the half-time of re-reduction of P700+ in the presence of DCMU after a pulse light under background far-red light was shortened by approximately 30%, indicating that cyclic electron flow around PS I was accelerated by the treatment. Based on these results it is suggested that the increase in photosynthesis in Synechocystis PCC6803 caused by low concentrations of NaHSO3 solution might be due to the stimulation of the cyclic electron flow around PS I and thus the increase in photophosphorylation.

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References

  • Allen M M (1968) Simple conditions for growth of unicellular bluegreen algae on plates. J Phycol 4: 1–4

    CAS  Google Scholar 

  • Asada K, Deura R and Kasai Z (1968) Effects of sulfate ions on photophosphorylation by spinach chloroplasts. Plant Cell Physiol 9: 143–146

    CAS  Google Scholar 

  • Asada K, Heber U and Schreiber U (1992) Pool size of electrons that can be donated to P700+ from stromal components via the intersystem chain. Plant Cell Physiol 33: 927–932

    CAS  Google Scholar 

  • Asada K, Heber U and Schreiber U (1993) Electron flow to the intersystem chain from stromal components and cyclic electron flow in maize chloroplasts, as detected in intact leaves by monitoring redox change of P700 and chlorophyll fluorescence. Plant Cell Physiol 34: 39–50

    CAS  Google Scholar 

  • Black VJ and Unsworth MH (1979a) A system for measuring effects of sulphur dioxide on gas exchange of plants. J Exp Bot 30: 81– 88

    CAS  Google Scholar 

  • Black VJ and Unsworth MH (1979b) Effects of low concentrations of sulphur dioxide on net photosynthesis and dark respiration of Vicia faba. J Exp Bot 30: 473–483

    CAS  Google Scholar 

  • Campbell D and Hurry V (1998) Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation. Microbial Mol Biol Rev 62: 667–683

    CAS  Google Scholar 

  • Cowling DW and Lockyer DR (1976) Growth of perennial ryegrass (Lolium perenne L.) exposed to low concentration of sulfur dioxide. J Exp Bot 27: 411–417

    CAS  Google Scholar 

  • Daniel M and Sarojini G (1981) On the possible site of sulfite action in the photosynthetic electron transport chain and the light modulation of enzyme activity. Photobiochem Photobiophys 2: 61–68

    Google Scholar 

  • Ferguson P (1979) The effects of sulphur pollutions in rain on the growth of Sphagnum species. PhD Thesis, University of Manchester, UK

    Google Scholar 

  • Ferguson P and Lee JA (1979) The effects of bisulphite and sulphate upon photosynthesis in Sphagnum. New Phytol 82: 703–712

    Article  CAS  Google Scholar 

  • Garab G, Lajko F, Mustardy L and Marton L (1989) Respiratory control over photosynthetic electron transport in chloroplasts of higher plant cells: evidence for chlororespiration. Planta 36: 349–358

    Article  Google Scholar 

  • Groom QJ, Kramer DM, Crofts AR and Ort DR (1993) The nonphotochemical reduction of plastoquinone in leaves. Photosynth Res 36: 205–215

    Article  CAS  Google Scholar 

  • Guo LW, Zhang Y and Shen YK (1994) Study on the method of feeding substance to leaves through petioles. Plant Physiol Commun 30: 360–362

    Google Scholar 

  • Herbert SK, Martin RE and Fork DC (1995) Light adaptation of cyclic electron transport through Photosystem I in the cyanobacterium Synechococcus sp. PCC 7942. Photosynth Res 46: 277–285

    Article  CAS  Google Scholar 

  • Hill DJ (1974) Some effects of sulphite on photosynthesis in lichens. New Phytol 73: 1193–1205

    Article  CAS  Google Scholar 

  • Jeanjean R, Thor JJ, Havaux M, Joset F and Matthijs HC (1999) Identification of plastoquinone-cytochrome b6 / f reductase pathways in direct or indirect Photosystem I driven cyclic electron flow in Synechocystis PCC6803. In: Peschek G, Loeffelhardt W and Schmellerer G (eds) The Phototrophic Prokaryotes, pp 251– 258. Kluwer Academic Publishers / Plenum Publishers, New York

    Google Scholar 

  • Jin MX, Mi HL, Ye JY and Shen YK (2000) Mathematical analysis of post-illumination transient increase in chlorophyll fluorescence and its dependence upon illumination duration in maize leaves. Acta Phytophysiol Sin 26: 219–226

    CAS  Google Scholar 

  • Kaneko T, Sato S and Kotani H (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. Strain PCC 6803. II. Sequence determination of the entire genome and assignment of potential protein–coding regions. DNA Res 3: 109–136

    Article  PubMed  CAS  Google Scholar 

  • Klughammer C and Schreiber U (1998) Measuring P700 absorbance changes in the near infrared spectral region with a dual wavelength pulse modulation system. In: Garab G (ed) Photosynthesis: Mechanism and Effects. Vol V, pp 4357–4360. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Maenpaa P, Kallio T, Mulo P, Salih G, Aro EM, Tyystjarvi E and Jansson C (1993) Site-specific mutations in the D1 polypeptide affect the susceptibility of Synechocystis 6803 cells to photoinhibition. Plant Mol Biol 22: 1–12

    Article  PubMed  CAS  Google Scholar 

  • Mano J, Miyake C, Schreiber U and Asada K (1995) Photoactivation of the electron flow from NADPH to plastoquinone in spinach chloroplasts. Plant Cell Physiol 36: 1589–1598

    CAS  Google Scholar 

  • Menser HA and Heggestad HE (1966) Ozone and sulfur dioxide synergism: injury to tobacco plants. Science 153: 424–425

    PubMed  Google Scholar 

  • Mi H, Endo T, Schreiber U, Ogawa T and Asada K (1992) Electron donation from cyclic and respiration flows to the photosynthetic intersystem chain is mediated by pyridine nucleotide dehydrogenase in the cyanobacterium Synechocystis PCC6803. Plant Cell Physiol 33: 1233–1237

    CAS  Google Scholar 

  • Mi H, Endo T, Ogawa T and Asada K (1995) Thylakoid membrane-bound NADPH-specific pyridine nucleotide dehydrogenase complex mediates cyclic transport in the cyanobacterium Synechocystis PCC6803. Plant Cell Physiol 36: 661–668

    CAS  Google Scholar 

  • Mi H, Deng Y, Tanaka Y, Hibino T and Takabe T (2001) Photoinduction of an NADPH dehydrogenase which functions as a mediator of electron transport to the intersystem chain in the cyanobacterium Synechocystis PCC6803. Photosynth Res 70: 167–173

    Article  PubMed  CAS  Google Scholar 

  • Mills JD, Slovacek RE and Hind G (1978) Cyclic electron transport in isolated intact chloroplasts. Further study with antimycin. Biochim Biophys Acta 504: 298–309

    Article  PubMed  CAS  Google Scholar 

  • Mills JD, Crowther D, Slovacek RE, Hind G and McCarty RE (1979) Electron transport pathway in spinach chloroplasts. Reduction of the primary acceptor of Photosystem II by reduced nicotinamide adenine dinucleotide phosphate in the dark. Biochim Biophys Acta 547: 127–137

    Article  PubMed  CAS  Google Scholar 

  • Pfanz H, Martinoia E, Lange OL and Heber U (1987) Flux of SO2 into leaf cells and cellular acidification by SO2. Plant Physiol 85: 928–933

    PubMed  CAS  Google Scholar 

  • Plesnièar Mand Kaleziæ R (1980) Sulfite inhibition of oxygen evolution associated with photosynthetic carbon assimilation. Period Biol 82: 297–301

    Google Scholar 

  • Puckett KJ, Nieboer E, Flora WP and Richardson DHS (1973) Sulphur dioxide: its effects on photosynthetic l4C fixation in lichens and suggested mechanisms of phytotoxicity. New Phytol 73: 141–154

    Article  Google Scholar 

  • Scherer S (1990) Do photosynthetic and respiratory electron transport chains share redox proteins? TIBS 15: 458–462

    PubMed  Google Scholar 

  • Scherer S, Almon H and Boeger P (1988) Interaction of photosynthesis, respiration and nitrogen fixation in cyanobacteria. Photosynth Res 15: 95–114

    Article  CAS  Google Scholar 

  • Schürmann P, Buchanan BB and Arnon DI (1972) Role of cyclic photophosphorylation in photosynthetic carbon dioxide assimilation by isolated chloroplasts. Biochim Biophys Acta 267: 111–124

    Article  PubMed  Google Scholar 

  • Shen YK and Wei JM (1998) The discovery of photophosphorylation. In: Kung SD and Yang SF (eds) Discoveries in Plant Biology, Vol II, pp 105–114. World Scientific, Singapore

    Google Scholar 

  • Shen YK, Li DY, Wei JMand Zhang XX (1980) Applied research on the method of improved dry weight to measure photosynthesis. Plant Physiol Commun 2: 37–41

    Google Scholar 

  • Shimazaki K, Sakaki T, Kondo N and Sugahara K (1980) Active oxygen participation in chlorophyll destruction and lipid peroxidation in SO2-fumigated leaves of spinach. Plant Cell Physiol 21: 1193–1204

    CAS  Google Scholar 

  • Slovacek RE and Hind G (1977) Influence of antimycin A and uncouplers on anaerobic photosynthesis in isolated chloroplasts. Plant Physiol 60: 538–542

    PubMed  CAS  Google Scholar 

  • Slovacek RE, Mills JD and Hind G (1978) The function of cyclic electron transport in photosynthesis. FEBS Lett 87: 73–76

    Article  CAS  Google Scholar 

  • Soldatini GF, Ziegler I and Ziegler H (1978) Sulfite: Preferential sulfur source and modifier of CO2 fixation in Chlorella vulgaris. Planta 143: 225–231

    CAS  Google Scholar 

  • Takemoto BK and Noble RD (1982) The effects of short-term SO2 fumigation on photosynthesis and respiration in soybean Glycine max. Environ Pollut Ser A 28: 67–74

    Article  CAS  Google Scholar 

  • Tan S and Shen YK (1987) The effects of sodium bisulfite on photosynthetic apparatus and its operation. Acta Phytophysiol Sin 13: 42–50

    CAS  Google Scholar 

  • Vermaas WFJ (1996) Molecular genetics of the cyanobacterium Synechocystis sp. PCC 6803: Principles and possible biotechnology applications. J Appl Phycol 8: 263–273

    Article  CAS  Google Scholar 

  • Vonshak A (1985) Culture methods and biomass produce of algae. In: Coombs J, Hall DO, Long SP and Scurlock JMO (eds) Techniques in Bioproductivity and Photosynthesis, 2nd edition, Vol 15, pp 196–211. Pergamon Press, Oxford

    Google Scholar 

  • Wang HW, Wei JM and Shen Y K (2000a) Enhancement of wheat leaf photophosphorylation and photosynthesis by spraying low concentration of NaHSO3. Chin Sci Bull 4: 1308–1311

    Article  Google Scholar 

  • Wang HW, Wei JM, Shen YK, Zhang RX and Yang TN (2000b) Enhancement of photophosphorylation and photosynthesis in rice by low concentrations of NaHSO3 under field conditions. Acta Bot Sin 42: 1295–1299

    CAS  Google Scholar 

  • Wei JM, Shen YK, Li DY and Xu CH (1989) Stimulatory effect of sodium bisulfite on cyclic photophosphorylation of chloroplasts under low light intensity. Acta Phytophysiol Sin 15: 101–104

    CAS  Google Scholar 

  • Wraight CA and Crofts AR (1971) Delayed fluorescence and highenergy state of chloroplasts. Eur J Biochem 19: 386–387

    Article  PubMed  CAS  Google Scholar 

  • Xu CH and Shen YK (1983) Relationship between the changes of the fast phase of millisecond delayed light emission and the proton released by the oxidation of water. Sci Sin Ser B 27: 37–47

    Google Scholar 

  • Xu BJ, Li DY and Shen YG (1989) The enhancement of low concentration of PMS on leaf's photosynthetic oxygen evolution in leaf. Acta Phytophysiol Sin 15: 307–312

    CAS  Google Scholar 

  • Ye JY, Mi HL, Wang YJ and Shen YK (1997) The transient postillumination increase in chloroplasts fluorescence in spinach thykoids. Chin Sci Bull 42: 1867–1870

    Google Scholar 

  • Yin HZ, Shen YK, Wang TD and Shi JN (1979) Research on operating photosynthetic machine. Acta Phytophysiol Sin 5: 295–317

    Google Scholar 

  • Zelitch I (1957) a-Hydroxysulfonates as inhibitors of the enzymatic oxidation of glycolic and lactic acids. J Biol Chem 224: 251–260

    PubMed  CAS  Google Scholar 

  • Zelitch I (1966) Increased rate of net photosynthetic carbon dioxide uptake caused by the inhibition of glycolate oxidase. Plant Physiol 41: 1623–1631

    Article  PubMed  CAS  Google Scholar 

  • Zhang XZ and Pang SQ (1984) The increase of soybean production by NaHSO3. Sci Agr Sin 1: 36–39

    Google Scholar 

  • Ziegler I (1975) The effect of SO2 pollution on plant metabolism. Residue Rev 56: 79–105

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, The People's Republic of China

    Hong-Wei Wang, Hualing Mi, Ji-Yu Ye, Yong Deng & Yun-Kang Shen

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  1. Hong-Wei Wang
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  2. Hualing Mi
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  3. Ji-Yu Ye
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  4. Yong Deng
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  5. Yun-Kang Shen
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Correspondence to Yun-Kang Shen.

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Wang, HW., Mi, H., Ye, JY. et al. Low concentrations of NaHSO3 increase cyclic photophosphorylation and photosynthesis in cyanobacterium Synechocystis PCC6803. Photosynthesis Research 75, 151–159 (2003). https://doi.org/10.1023/A:1022813402265

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