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Expression of FC∈II/CD23 on Human Neutrophils Isolated from Rheumatoid Arthritis Patients

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Abstract

CD23, the low affinity receptor for IgE, is a 45 kilodalton molecule belonging to the C-type lectin family, some members of which have been identified as adhesion molecules. Since it has been described upregulated in different cells in chronic inflammatory diseases and in rheumatoid arthritis in particular, where neutrophils are directly involved in tissue damage, our interest, in this work, has been focused on the expression and regulation of this antigen on neutrophil membrane. We studied 22 patients suffering from rheumatoid arthritis and 22 healthy control subjects. CD23 expression on neutrophil membrane was analyzed by immunofluorescence. Neutrophils of 9 out of 22 patients expressed CD23 molecules, neutrophils of 11 out of 22 patients expressed CD23 only after 24 h of incubation in RPMI; only 2 out of 22 patients did not express the CD23 antigen on neutrophil membrane either after isolation or after a 24 h incubation. On the contrary neutrophils isolated from healthy subjects did not express CD23 molecules upon isolation. Only in 7/22 control subjects neutrophils resulted positive after 24 h of incubation in RPMI. Moreover, we found that in our experimental conditions the presence of IFN-g or GM-CSF alone or in combination with IL-4 inhibited CD23 expression during the 24 h incubation. Our results show that there is a strong association between neutrophil ability to express CD23 and rheumatoid arthritis, and that such expression may be regulated by GM-CSF, IFN-γ and IL-4.

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REFERENCES

  1. Feldmann, M., F. M. Brennan, and R. N. Maini. 1996. Rheumatoid arthritis. Cell. 85:307-310.

    Google Scholar 

  2. Bonnefoy, J. Y., C. Plater-Zyberk, S. Lecoanet-Henchoz, J. F. Gauchat, J. P. Aubry, and P. Graber. 1996. A new role for CD23 in inflammation. Immunol. Today. 17:418-420.

    Google Scholar 

  3. Delespesse, G., C. Sarati, C. Y. Wu, S. Fournier, and M. Letellier. 1992. The low-affinity receptor for IgE. Immunol. Rev. 125:77-97.

    Google Scholar 

  4. Conrad, D. H. 1989. FCεRII/CD23: The Low Affinity receptor for IgE. Ann. Rev. Immunol. 8:623-645.

    Google Scholar 

  5. Stoolman, L. M. 1989. Adhesion molecules controlling lymphocyte migration. Cell. 56:907.

    Google Scholar 

  6. Aubry, J. P., S. Pochon, P. Graber, K. Jansen, and J. Y. Bonnefoy. 1992. CD21 is a ligand for CD23 and regulates IgE production. Nature. 358:505-507.

    Google Scholar 

  7. Lecoanet-Henchoz, S., J. P. Gauchat, J. P. Aubry, P. Graber, P. Life, B. Ferrua, A. L. Corbi, B. Dugas, C. Plater-Zyberk, and J. Y. Bonnefoy. 1995. CD23 regulates monocyte activation through a novel interaction with the adhesion molecules CD11b and CD18 and CD11c-CD18. Immunity 3:119-125.

    Google Scholar 

  8. Kehry, M. R., and L. C. Yamishita. 1989. FCΣ receptor II (CD23) function on mouse B cells: role in IgE dependent antigen focusing. Proc. Natl. Acad. Sci. USA. 86:7556-7560.

    Google Scholar 

  9. Capron, M., M. D. Kazatchkine, E. Fisher, M. Joseph, A. E. Butterworth, J. P. Kusnierz, L. Prin, J. P. Papin, and A. Capron. 1987. Functional role of the alpha-chain of complement receptor type 3 in human eosinophil-dependent antibody-mediated cytotoxicity against schistosomes. J. Immunol. 139:2059-2065.

    Google Scholar 

  10. Vouldoukis, I., V. Riveros-Moreno, B. Dugas, F. Ouaaz, P. BÈcherel, P. DebrÈ, S. Moncada, and M. D. Mossalayi. 1995. The killing of Leishmania major by human macrophages is mediated by nitric oxide induced after ligation of the FceRII/CD23 antigen. Proc. Natl. Acad. Sci. USA. 92:7804-7808.

    Google Scholar 

  11. Capron, A., J. P. Dessaint, M. Capron, M. Jospeh, J. C. Ameisen, and A. B. Tonnel. 1986. From parasites to allergy: a second receptor for IgE. Immunol. Today. 7:15.

    Google Scholar 

  12. Borish, L., J. J. Mascali, and L. J. Rosenwasser. 1991. IgE-dependent cytokine production by human peripheral blood mononuclear phagocytes. J. Immunol. 146:63-67.

    Google Scholar 

  13. Dugas, B., M. D. Mossalayi, C. Damais, and J. P. Kolb. 1995. Nitric oxide production by human monocytes: evidence for a role of CD23. Immunol. Today. 16:574-580.

    Google Scholar 

  14. Lee, B. W., C. F. Simmons, T. Wileman, and R. S. Geha. 1989. Intracellular cleavage of newly synthesized low affinity Fce receptor (FceRII) provides a second pathway for the generation of the 28-kDa soluble FceR2 fragment. J. Immunology 142:1614-1620.

    Google Scholar 

  15. Yukawa, K., H. Kikutani, H. Owaki, K. Yamasaki, A. Yokota, H. Nakamura, E. L. Barsumian, R. R. Hardy, M. Suemura, and T. Kishimoto. 1987. A B cell-specific differentiation antigen, CD23, is a receptor for IgE (Fc epsilon R) on lymphocytes. J. Immunol. 138:2576-2580.

    Google Scholar 

  16. Kikutani, H., A. Yokota, N. Uchibayashi, K. Yakawa, T. Tanaka, K. Sugiyama, E. L. Barsumina, M. Suemura, and T. Kishimoto. 1989. Structure and function of Fc epsilon receptor II (Fc epsilon RII/CD23): a point of contact between the effector phase of allergy and B cell differentiation. Ciba Found. Symp. 147:23-31.

    Google Scholar 

  17. Truong, M. J., V. Gruart, J. P. Kusinerz, J. P. Papin, S. Loiseau, A. Capron, and M. Capron. 1993. Human neutrophils express immunoglobulin E (IgE)-binding proteins (Mac2/eBP) of the S-type lectin family: role in IgE-dependent activation. J. Exp. Med. 177:243-248.

    Google Scholar 

  18. Yamaoka, K. A., M. Arock, F. Issaly, N. Dugas, L. Le Goff, and J. P. Kolb. 1996. Granulocyte macrophage colony stimulating factor induces FcεRII/CD23 expression on normal human polymorphonuclear neutrophils. Int. Immunol. 8:479-490.

    Google Scholar 

  19. Hellen, E. A., D. C. Rowlands, T. T. Hansel, G. D. Kitas, and J. Crocker. 1991. Immunohistochemical demonstration of CD23 expression on lymphocytes in rheumatoid synovitis. J. Clin. Pathol. 44:293-296.

    Google Scholar 

  20. Arnett, F. C., S. M. Edworthy, D. A. Bloch, D. J. McShane, J. F. Fries, N. S. Cooper, L. A. Healey, S. R. Kaplan, M. H. Liang, H. S. Luthra, T. A. Medsger, Jr., D. M. Mitchell, D. H. Neustadt, R. S. Pinals, J. C. Schaller, J. T. Sharp, R. L. Wilder, and G. G. Hunder. 1988. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 31:315-324.

    Google Scholar 

  21. Bellavite, P., S. Chirumbolo, C. Mansoldo, G. Gandini, and P. Dri. 1992. Simultaneous assay for oxidative metabolism and adhesion of human neutrophils: evidence for correlations and dissociations of the two responses. J. Leukoc. Biol. 51:329-335.

    Google Scholar 

  22. Hansel, T. T., J. D. Pound, and D. Pilling. 1989. Purification of human blood eosinophils by negative selection using immunomagnetic beads. J. Immunol. Methods. 122:97-103.

    Google Scholar 

  23. Hogg, N. 1987. Leucocyte Typing III, 576-602. Oxford University Press.

  24. Bansal, A. S., A. J. Macgregor, R. S. Pumphrey, A. J. Silman, W. E. Ollier, and P. B. Wilson. 1994. Increased level of SCD23 in Rheumatoid Arthritis are related to disease status. Clin. Exp. Rheumatol. 12:281-285.

    Google Scholar 

  25. TeVelde, A. A., F. Rousset, C. Peronne, J. E. De Vries, and C. G. Figdor. 1990. IFN-alpha and IFN-gamma have different regulatory effects on IL-4-induced membrane expression of Fc epsilon RIIb and release of soluble Fc epsilon RIIb by human monocytes. J. Immunol. 144:3052-3059.

    Google Scholar 

  26. Plater-Zyberk, C., and J. Y. Bonnefoy. 1995. Marked amelioration of established collagen-induced arthritis by treatment with antibodies to CD23 in vivo. Nat. Med. 1:781-785.

    Google Scholar 

  27. Dugas, B., M. D. Mossalayi, C. Damais, and J. P. Kolb. 1995. Nitric oxide production by human monocytes: evidence for a role of CD23. Immunol. Today. 16:574-580.

    Google Scholar 

  28. Mudde, C. G., T. T. Hansel, F. C. Von Reijsen, B. F. Osterhoff, and C. A. Bruijnzeel-Koomen. 1990. IgE: An immunoglobulin specialized in antigen capture? Immunol. Today. 11:440-443.

    Google Scholar 

  29. Bonnefoy, J. Y., O. Guillot, H. Spits, D. Blanchard, K. Ishizaka, and J. Banchereau. 1988. The low-affinity receptor for IgE (CD23) on B lymphocytes is spatially associated with HLA-DR antigens. J. Exp. Med. 167:57-72.

    Google Scholar 

  30. Write, C. D., A. Mulsch, R. Busse, and H. Osswald. 1989. Generation of Nitric Oxide by human neutrophils. Biochem. Biophys. Res. Commun. 160:813-819.

    Google Scholar 

  31. Evans, T. J., L. D. Buttery, A. Carpenter, D. R. Springall, J. M. Polak, and J. Cohen. 1996. Cytokine treated human neutrophils contain inducible nitric oxide synthase that produces nitration of ingested bacteria. PNAS U.S.A. 93:9553-9558.

    Google Scholar 

  32. Gosselin, E. J., K. Wardwell, W. F. Rigby, and P. M. Guyre. 1993. Induction of MHC class II on human polymorphonuclear neutrophils by granulocyte/macrophage colony-stimulating factor, IFN-gamma and IL-3. J. Immunol. 151:1482-1490.

    Google Scholar 

  33. Fanger, N. A., C. Liu, P. M. Guyre, K. Wardwell, J. O'Neil, T. L. Guo, T. P. Christian, S. P. Mudzinski, and E. J. Gosselin. 1997. Activation of human T cells by major histocompatibility complex class II expressing neutrophils: proliferation in the presence of superantigens, but not tetanus toxoid. Blood. 89:4128-4135.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Immunology and Infectious Diseases, Italy

    Antonio Vella, Alessandra Adami, Riccardo Ortolani & Giuseppe Tridente

  2. Institute of Pharmacology, University of Verona, Yia Delle Menegone, 37100, Verona, Italy

    Giuseppina Benoni

  3. Department of Clinical and Experimental Medicine, University of Verona, Yia Delle Menegone, 37100, Verona, Italy

    Antonio Carletto, Domenico Biasi & Paola Caramaschi

  4. Clinical Chemistry and Microscopy, University of Verona, Yia Delle Menegone, 37100, Verona, Italy

    Paolo Bellavite

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  1. Antonio Vella
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Vella, A., Bellavite, P., Adami, A. et al. Expression of FC∈II/CD23 on Human Neutrophils Isolated from Rheumatoid Arthritis Patients. Inflammation 23, 471–479 (1999). https://doi.org/10.1023/A:1021969127489

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