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Gelatinase A (MMP-2), Collagenase-2 (MMP-8), and Laminin-5 γ2-Chain Expression in Murine Inflammatory Bowel Disease (Ulcerative Colitis)

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Abstract

Dextran sulfate sodium-induced inflammatory bowel disease in mice resembles human ulcerative colitis. In inflammatory bowel diseases matrix metalloproteinases contribute to tissue degradation. Laminin-5 is an anchoring filament protein in the basement membrane area that can be cleaved by matrix metalloproteinases. We investigated the expression of matrix metalloproteinases-2 and -8 and laminin-5 γ2-chain in dextran sulfate sodium-induced mice by immunohistochemistry and in situ hybridization. Matrix metalloproteinase-8 expression was evidenced in the colon surface epithelial cells and the protein was more abundant in dextran sulfate sodium-induced mice colon. Matrix metallproteinase-2 and laminin-5 γ2-chain colocalized in the colon surface epithelial cells and in the basement membrane zone as demonstrated by double immunostaining. In dextran sulfate sodium-induced colon, matrix metalloproteinase-2 immunoreactivity was detected in epithelial cells in the lower parts of the crypt and surrounding the degraded crypts. Matrix metalloproteinase-2 and -8 could participate in the local epithelial inflammatory processes and tissue destruction. The presence of laminin-5 γ2-chain indicates alternative anchoring mechanisms in the colon, a compartment devoid of hemidesmosomes.

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Authors and Affiliations

  1. Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Finland

    Emma Pirilä

  2. Oral Pathology Unit, Institute of Dentistry, Biomedicum Helsinki, Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital (HUCH), University of Helsinki, Orton Research Institute and the Orthopedic Hospital of the Invalid Foundation, Helsinki, Finland

    Emma Pirilä

  3. SUNY at Stony Brook, School of Dental Medicine, New York, USA

    Nungavarm S. Ramamurthy

  4. Oral Pathology Unit, Institute of Dentistry, Biomedicum Helsinki, Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital (HUCH), University of Helsinki, Orton Research Institute and the Orthopedic Hospital of the Invalid Foundation, Helsinki, Finland

    Timo Sorsa & Jarkko Hietanen

  5. Laboratory Diagnostics, Helsinki University Central Hospital (HUCH), Helsinki, Finland

    Timo Sorsa

  6. Institute of Dentistry, University of Oulu, Finland

    Tuula Salo

  7. Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, University of Helsinki, Orton Research Institute and the Orthopedic Hospital of the Invalid Foundation, Helsinki, Finland

    Päivi Maisi

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  1. Emma Pirilä
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Pirilä, E., Ramamurthy, N.S., Sorsa, T. et al. Gelatinase A (MMP-2), Collagenase-2 (MMP-8), and Laminin-5 γ2-Chain Expression in Murine Inflammatory Bowel Disease (Ulcerative Colitis). Dig Dis Sci 48, 93–98 (2003). https://doi.org/10.1023/A:1021790532723

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