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Delayed Hypothermia Prevents Decreases in N-Acetylaspartate and Reduced Glutathione in the Cerebral Cortex of the Neonatal Pig Following Transient Hypoxia-Ischaemia

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Abstract

The effects of normothermia and delayed hypothermia on the levels of N-acetylaspartate (NAA), reduced glutathione (GSH) and the activities of mitochondrial complex I, II-III, IV and citrate synthase were measured in brain homogenates obtained from anaesthetized neonatal pigs following transient in vivo hypoxia-ischaemia. In the normothermic animals there was a significant decrease in complex I activity and in the levels of GSH and NAA when compared to the controls. Delayed hypothermia preserved NAA and GSH at control levels and enhanced the rate of complex II-III activity. There was correlation (R = 0.79) between GSH and NAA levels when data from all three experimental groups were analyzed. Citrate synthase activity was not significantly different in the three groups, indicating maintenance of mitochondrial integrity. These data suggest that delayed hypothermia affords protection of integrated mitochondrial function in the neonatal brain following transient hypoxia-ischaemia.

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Authors and Affiliations

  1. Department of Paediatrics, University College London Medical School, London, WC1E 6JJ, United Kingdom

    Keith J. Brooks, Mark Sellwood, Francis O'Brien, Martina Noone & Yasuko Sakata

  2. Department of Clinical Biochemistry, National Hospital for Neurology and Neurosurgery, Queen Square, London, WC1N 3BG, United Kingdom

    Iain Hargreaves

  3. Robert Steiner MR Unit, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College of Science, Technology and Medicine Hammersmith Campus Du Cane Road, London, W12 ONN, United Kingdom

    Kishore Bhakoo

  4. Department of Medical Physics and Bioengineering, University College Hospital NHS Trusts, 1st Floor, Shropshire House, 11-20 Capper Street, London, WC1E 6JA, United Kingdom

    Ernest Cady, Marzena Wylezinska, John Thornton, Roger Ordidge, Quyen Nguyen, Matthew Clemence & John Wyatt

  5. MRC Applied Neuroscience Group, School of Biomedical Sciences, University of Nottingham Medical School, Queens Medical Centre, Nottingham, NG7 2UH, United Kingdom

    Timothy E. Bates

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  1. Keith J. Brooks
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Brooks, K.J., Hargreaves, I., Bhakoo, K. et al. Delayed Hypothermia Prevents Decreases in N-Acetylaspartate and Reduced Glutathione in the Cerebral Cortex of the Neonatal Pig Following Transient Hypoxia-Ischaemia. Neurochem Res 27, 1599–1604 (2002). https://doi.org/10.1023/A:1021622724485

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