Abstract
The effects of various stresses (osmotic, salt, low-temperature, high-temperature, and high-light stress) on the amount of mRNA of eight genes encoding the secreted proteins of Synechocystis sp. PCC 6803 were studied. Osmotic stress (0.5 M sorbitol) reduced the amount of all mRNAs, with the exception of slr0924. Supposedly, this gene encodes Tic22, a polypeptide involved in the formation of the transport system for proteins crossing the internal thylakoid membrane on the way to the lumen. Salt stress (0.5 M NaCl) inhibited the expression of all genes for secreted proteins almost completely. Low temperature (20°C) did not affect the expression of the sll1891 gene of an unknown function and the slr0924 gene. The high temperature (44°C) suppressed the expression of all genes tested. A detailed study of the expression of the sll1694 (pilA1) gene, which encodes the main structural protein of cyanobacterial pili, pilin PilA1, demonstrated that virtually all stresses suppressed its expression. Thus, various stresses were shown to suppress the expression of most genes encoding Synechocystis secreted proteins.
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REFERENCES
Hill, D.R., Hladun, S.L., Scherer, S., and Potts, M., Water Stress Proteins of Nostoc commune (Cyanobacteria) Are Secreted with UV-A/B-Absorbing Pigments and Associate with 1,4-β-D-Xylanxylanohydrolase Activity, J. Biol. Chem., 1994, vol. 269, pp. 7726-7734.
Takahashi, I., Hayano, D., Asayama, M., Masahiro, F., Watahiki, M., and Shirai, M., Restriction Barrier Composed of an Extracellular Nuclease and Restriction Endonuclease in the Unicellular Cyanobacterium Microcystis sp., FEMS Microbiol. Lett., 1996, vol. 145, pp. 107-111.
Engels, A., Kahmann, U., Ruppel, H.G., and Pistorius, E.K., Isolation, Partial Characterization and Localization of a Dihydrolipoamide Dehydrogenase from the Cyanobacterium Synechocystis PCC 6803, Biochim. Biophys. Acta, 1997, vol. 1340, pp. 33-44.
Hoiczyk, E. and Baumeister, W., Oscillin, an Extracellular, Ca2+-Binding Glycoprotein Essential for the Gliding Motility of Cyanobacteria, Mol. Microbiol., 1997, vol. 26, pp. 699-708.
Muro-Pastor, A.M., Herrero, A., and Flores, E., The nuiA Gene from Anabaena sp. Encoding an Inhibitor of the NucA Sugar-Non-Specific Nuclease, J. Mol. Biol., 1997, vol. 268, pp. 589-598.
Sergeyenko, T.V. and Los, D.A., Identification of Secreted Proteins of the Cyanobacterium Synechocystis sp. Strain PCC 6803, FEMS Microbiol. Lett., 2000, vol. 193, pp. 213-216.
Bhaya, D., Watanabe, N., Ogawa, T., and Grossman, A.R., The Role of an Alternative Sigma Factor in Motility and Pilus Formation in the Cyanobacterium Synechocystis sp. PCC 6803, Proc. Natl. Acad. Sci. USA, 1999, vol. 96, pp. 3188-3193.
Bhaya, D., Bianko, N.R., Bryant, D., and Grossman, A., Type IV Pilus Biogenesis and Motility in the Cyanobacterium Synechocystis sp. PCC 6803, Mol. Microbiol., 2000, vol. 37, pp. 941-951.
Yoshihara, S., Geng, X.X., Okamoto, S., Yura, K., Murata, T., Go, M., Ohmori, M., and Ikeuchi, M., Mutational Analysis of Genes Involved in Pilus Structure, Motility and Transformation Competency in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803, Plant Cell Physiol., 2001, vol. 42, pp. 63-73.
Stanier, R.Y., Kunisawa, R., Mandel, M., and Cohen-Bazire, G., Purification and Properties of Unicellular Blue-Green Algae (Order Chroococcales), Bacteriol. Rev., 1971, vol. 35, pp. 171-205.
Los, D.A., Horvath, I., Vigh, L., and Murata, N., The Temperature-Dependent Expression of the Desaturase Gene desA in Synechocystis PCC 6803, FEBS Lett., 1993, vol. 378, pp. 57-60.
Kiseleva, L.L., Serebriiskaya, T.S., Horvath, I., Vigh, L., Lyukevich, A.A., and Los, D.A., Expression of the Gene for the Δ9 Acyl-Lipid Desaturase in the Thermophilic Cyanobacterium, J. Mol. Microbiol. Biotechnol., 2000, vol. 2, pp. 331-338.
Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., Kimura, T., Hosouchi, T., Matsuno, A., Muraki, A., Nakazaki, N., Naruo, K., Okumura, S., Shimpo, S., Takeuchi, C., Wada, T., Watanabe, A., Yamada, M., Yasuda, M., and Tabata, S., Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC 6803: 2. Sequence Determination of the Entire Genome and Assignment of Potential Protein-Coding Regions, DNA Res., 1996, vol. 3, pp. 109-136.
Yoshihara, S., Suzuki, F., Fujita, H., Geng, X.X., and Ikeuchi, M., Novel Putative Photoreceptor and Regulatory Genes Required for the Positive Phototactic Movement of the Unicellular Cyanobacterium Synechocystis.sp. PCC 6803, Plant Cell Physiol., 2000, vol. 41, pp. 1299-1304.
Suzuki, I., Los, D.A., Kanesaki, Y., Mikami, K., and Murata, N., The Pathway for Perception and Transduction of Low-Temperature Signals in Synechocystis, EMBO J., 2000, vol. 19, pp. 1327-1334.
Strom, M.S. and Lory, S., Structure—Function and Biogenesis of the Type IV Pili, Annu. Rev. Microbiol., 1993, vol. 47, pp. 565-596.
Los, D.A., Ray, M.K., and Murata, N., Differences in the Control of the Temperature-Dependent Expression of Four Genes for Desaturases in Synechocystis sp. PCC 6803, Mol. Microbiol., 1997, vol. 25, pp. 1167-1176.
Lehel, C., Los, D.A., Wada, H., Gyorgyey, A., Horvath, I., Kovacs, E., Murata, N., and Vigh, L., A Second groEL-Like Gene Organized in a groESL Operon Is Present in the Genome of Synechocystis sp. PCC 6803, J. Biol. Chem., 1993, vol. 268, pp. 1799-1804.
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Sergeyenko, T.V., Los, D.A. The Effect of Various Stresses on the Expression of Genes Encoding the Secreted Proteins of the Cyanobacterium Synechocystis sp. PCC 6803. Russian Journal of Plant Physiology 49, 650–656 (2002). https://doi.org/10.1023/A:1020293019304
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DOI: https://doi.org/10.1023/A:1020293019304