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Origin and Progression of Pregnancy-Dependent Mammary Tumors Induced by New Mouse Mammary Tumor Virus Variants

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Abstract

In order to study mechanisms of progression of mouse mammary tumor virus (MMTV)-induced pregnancy-dependent mammary lesions, we removed and serially transplanted 17 small tumors detected in MMTV-infected pregnant females. This gave rise to the same number of 'in vivo' tumor lines. Hormone-dependency of the passages was determined by comparing tumor development in multiparous versus virgin hosts. We found that the first passages of most of these lesions (11/17) required pregnancy to grow. However, all these tumor lines lost their hormone-dependence through successive passages. The original pregnancy-dependent lesions were mostly multiclonal and showed high levels of estrogen and progesterone receptors. Alternatively, pregnancy-independent tumors arose as clonal dominant populations exhibiting a lower hormone receptor content. Our data show that the progression of hormone-dependent MMTV-induced mammary tumors is an irreversible process associated with the appearance of additional MMTV insertional events as well as alterations in the composition of the tumor cell population.

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Authors and Affiliations

  1. ILEX-CONICET, División de Medicina Experimental, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Argentina

    Valeria Buggiano, Carolina Schere Levy, Albana Gattelli, María Cecilia Cirio, Mariana Marfil, Irene Nepomnaschy, Isabel Piazzon & Edith C. Kordon

  2. IBYME (Instituto de Biología y Medicina Experimental)-CONICET, Argentina

    Luisa Helguero

  3. Centro de Estudios Oncológicos, Academia Nacional de Medicina, Buenos Aires, Argentina

    Silvia Vanzulli

Authors
  1. Valeria Buggiano
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  2. Carolina Schere Levy
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  3. Albana Gattelli
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  4. María Cecilia Cirio
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  5. Mariana Marfil
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  6. Irene Nepomnaschy
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  7. Isabel Piazzon
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  8. Luisa Helguero
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  9. Silvia Vanzulli
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  10. Edith C. Kordon
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Buggiano, V., Schere Levy, C., Gattelli, A. et al. Origin and Progression of Pregnancy-Dependent Mammary Tumors Induced by New Mouse Mammary Tumor Virus Variants. Breast Cancer Res Treat 75, 191–202 (2002). https://doi.org/10.1023/A:1019932516887

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  • DOI: https://doi.org/10.1023/A:1019932516887

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